CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/420.mrc Output diagnostic file name Micrographs_0601/420.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/420.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2290.000 10066.00 Mean, RMS : 4562.954 223.4839 TITLE 1: emi2mrc: 420.emi Mon Jan 06 14:12:06 2014 TITLE 2: kV 300, Mag 78000x, Def 21660, Spot 4, Int 55.05%, Obj 88.05%, Dif 73.67% TITLE 3: X 299.731, Y 846.608, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01251 10200.00 10000.00 0.00 -0.01243 10400.00 10000.00 0.00 -0.01228 10600.00 10000.00 0.00 -0.01218 10800.00 10000.00 0.00 -0.01197 11000.00 10000.00 0.00 -0.01172 11200.00 10000.00 0.00 -0.01155 11400.00 10000.00 0.00 -0.01139 11600.00 10000.00 0.00 -0.01117 11800.00 10000.00 0.00 -0.01092 12000.00 10000.00 0.00 -0.01083 12200.00 10000.00 0.00 -0.01062 12400.00 10000.00 0.00 -0.01040 12600.00 10000.00 0.00 -0.01036 12800.00 10000.00 0.00 -0.01015 13000.00 10000.00 0.00 -0.00990 13200.00 10000.00 0.00 -0.00972 13400.00 10000.00 0.00 -0.00937 13600.00 10000.00 0.00 -0.00894 13800.00 10000.00 0.00 -0.00847 14000.00 10000.00 0.00 -0.00788 14200.00 10000.00 0.00 -0.00733 14400.00 10000.00 0.00 -0.00689 14600.00 10000.00 0.00 -0.00646 14800.00 10000.00 0.00 -0.00586 15000.00 10000.00 0.00 -0.00495 15200.00 10000.00 0.00 -0.00393 15400.00 10000.00 0.00 -0.00297 15600.00 10000.00 0.00 -0.00185 15800.00 10000.00 0.00 -0.00053 16000.00 10000.00 0.00 0.00075 16200.00 10000.00 0.00 0.00186 16400.00 10000.00 0.00 0.00287 16600.00 10000.00 0.00 0.00406 16800.00 10000.00 0.00 0.00537 17000.00 10000.00 0.00 0.00667 17200.00 10000.00 0.00 0.00799 17400.00 10000.00 0.00 0.00920 17600.00 10000.00 0.00 0.01032 17800.00 10000.00 0.00 0.01146 18000.00 10000.00 0.00 0.01256 18200.00 10000.00 0.00 0.01381 18400.00 10000.00 0.00 0.01520 18600.00 10000.00 0.00 0.01644 18800.00 10000.00 0.00 0.01772 19000.00 10000.00 0.00 0.01903 19200.00 10000.00 0.00 0.02031 19400.00 10000.00 0.00 0.02161 19600.00 10000.00 0.00 0.02298 19800.00 10000.00 0.00 0.02451 20000.00 10000.00 0.00 0.02616 20200.00 10000.00 0.00 0.02800 20400.00 10000.00 0.00 0.02997 20600.00 10000.00 0.00 0.03202 20800.00 10000.00 0.00 0.03413 21000.00 10000.00 0.00 0.03634 21200.00 10000.00 0.00 0.03843 21400.00 10000.00 0.00 0.04010 21600.00 10000.00 0.00 0.04134 21800.00 10000.00 0.00 0.04191 22000.00 10000.00 0.00 0.04210 22200.00 10000.00 0.00 0.04214 21800.00 10200.00 0.00 0.04229 22000.00 10200.00 0.00 0.04245 22200.00 10200.00 0.00 0.04252 21800.00 10400.00 0.00 0.04277 22000.00 10400.00 0.00 0.04297 22200.00 10400.00 0.00 0.04308 21800.00 10600.00 0.00 0.04345 22000.00 10600.00 0.00 0.04369 22200.00 10600.00 0.00 0.04380 21800.00 10800.00 0.00 0.04411 22000.00 10800.00 0.00 0.04434 22200.00 10800.00 0.00 0.04439 21600.00 11000.00 0.00 0.04440 21800.00 11000.00 0.00 0.04494 22000.00 11000.00 0.00 0.04513 22200.00 11000.00 0.00 0.04513 21800.00 11200.00 0.00 0.04561 22000.00 11200.00 0.00 0.04578 22200.00 11200.00 0.00 0.04579 21800.00 11400.00 0.00 0.04629 22000.00 11400.00 0.00 0.04646 22200.00 11400.00 0.00 0.04648 21800.00 11600.00 0.00 0.04695 22000.00 11600.00 0.00 0.04711 22200.00 11600.00 0.00 0.04715 21800.00 11800.00 0.00 0.04753 22000.00 11800.00 0.00 0.04769 22200.00 11800.00 0.00 0.04774 21600.00 12000.00 0.00 0.04780 21800.00 12000.00 0.00 0.04834 22000.00 12000.00 0.00 0.04851 22200.00 12000.00 0.00 0.04857 21600.00 12200.00 0.00 0.04864 21800.00 12200.00 0.00 0.04919 22000.00 12200.00 0.00 0.04937 22200.00 12200.00 0.00 0.04942 21600.00 12400.00 0.00 0.04951 21800.00 12400.00 0.00 0.05008 22000.00 12400.00 0.00 0.05025 22200.00 12400.00 0.00 0.05028 21600.00 12600.00 0.00 0.05054 21800.00 12600.00 0.00 0.05109 22000.00 12600.00 0.00 0.05123 22200.00 12600.00 0.00 0.05124 21600.00 12800.00 0.00 0.05154 21800.00 12800.00 0.00 0.05209 22000.00 12800.00 0.00 0.05221 22200.00 12800.00 0.00 0.05223 21600.00 13000.00 0.00 0.05255 21800.00 13000.00 0.00 0.05310 22000.00 13000.00 0.00 0.05323 22200.00 13000.00 0.00 0.05326 21600.00 13200.00 0.00 0.05370 21800.00 13200.00 0.00 0.05422 22000.00 13200.00 0.00 0.05432 21600.00 13400.00 0.00 0.05476 21800.00 13400.00 0.00 0.05520 22000.00 13400.00 0.00 0.05525 21600.00 13600.00 0.00 0.05596 21800.00 13600.00 0.00 0.05633 22000.00 13600.00 0.00 0.05635 21600.00 13800.00 0.00 0.05735 21800.00 13800.00 0.00 0.05762 21600.00 14000.00 0.00 0.05854 21800.00 14000.00 0.00 0.05876 21600.00 14200.00 0.00 0.05966 21800.00 14200.00 0.00 0.05989 22000.00 14200.00 0.00 0.05991 21600.00 14400.00 0.00 0.06069 21800.00 14400.00 0.00 0.06094 22000.00 14400.00 0.00 0.06098 21600.00 14600.00 0.00 0.06185 21800.00 14600.00 0.00 0.06213 22000.00 14600.00 0.00 0.06217 21600.00 14800.00 0.00 0.06308 21800.00 14800.00 0.00 0.06337 22000.00 14800.00 0.00 0.06340 21600.00 15000.00 0.00 0.06429 21800.00 15000.00 0.00 0.06458 22000.00 15000.00 0.00 0.06459 21600.00 15200.00 0.00 0.06566 21800.00 15200.00 0.00 0.06592 21600.00 15400.00 0.00 0.06704 21800.00 15400.00 0.00 0.06728 21400.00 15600.00 0.00 0.06732 21600.00 15600.00 0.00 0.06849 21800.00 15600.00 0.00 0.06873 21400.00 15800.00 0.00 0.06881 21600.00 15800.00 0.00 0.07000 21800.00 15800.00 0.00 0.07024 21400.00 16000.00 0.00 0.07043 21600.00 16000.00 0.00 0.07162 21800.00 16000.00 0.00 0.07183 21400.00 16200.00 0.00 0.07204 21600.00 16200.00 0.00 0.07324 21800.00 16200.00 0.00 0.07343 21400.00 16400.00 0.00 0.07369 21600.00 16400.00 0.00 0.07489 21800.00 16400.00 0.00 0.07508 21400.00 16600.00 0.00 0.07537 21600.00 16600.00 0.00 0.07657 21800.00 16600.00 0.00 0.07675 21400.00 16800.00 0.00 0.07708 21600.00 16800.00 0.00 0.07830 21800.00 16800.00 0.00 0.07844 21400.00 17000.00 0.00 0.07880 21600.00 17000.00 0.00 0.07999 21800.00 17000.00 0.00 0.08008 21400.00 17200.00 0.00 0.08047 21600.00 17200.00 0.00 0.08162 21800.00 17200.00 0.00 0.08167 21400.00 17400.00 0.00 0.08223 21600.00 17400.00 0.00 0.08335 21800.00 17400.00 0.00 0.08336 21400.00 17600.00 0.00 0.08407 21600.00 17600.00 0.00 0.08515 21400.00 17800.00 0.00 0.08621 21600.00 17800.00 0.00 0.08722 21400.00 18000.00 0.00 0.08831 21600.00 18000.00 0.00 0.08927 21400.00 18200.00 0.00 0.09042 21600.00 18200.00 0.00 0.09134 21400.00 18400.00 0.00 0.09256 21600.00 18400.00 0.00 0.09345 21400.00 18600.00 0.00 0.09465 21600.00 18600.00 0.00 0.09549 21400.00 18800.00 0.00 0.09673 21600.00 18800.00 0.00 0.09750 21400.00 19000.00 0.00 0.09865 21600.00 19000.00 0.00 0.09937 21400.00 19200.00 0.00 0.10087 21600.00 19200.00 0.00 0.10154 21400.00 19400.00 0.00 0.10323 21600.00 19400.00 0.00 0.10384 21400.00 19600.00 0.00 0.10567 21600.00 19600.00 0.00 0.10623 21400.00 19800.00 0.00 0.10823 21600.00 19800.00 0.00 0.10872 21200.00 20000.00 0.00 0.10894 21400.00 20000.00 0.00 0.11089 21600.00 20000.00 0.00 0.11124 21200.00 20200.00 0.00 0.11177 21400.00 20200.00 0.00 0.11364 21600.00 20200.00 0.00 0.11385 21200.00 20400.00 0.00 0.11465 21400.00 20400.00 0.00 0.11642 21600.00 20400.00 0.00 0.11642 21200.00 20600.00 0.00 0.11777 21400.00 20600.00 0.00 0.11934 21200.00 20800.00 0.00 0.12044 21400.00 20800.00 0.00 0.12178 21200.00 21000.00 0.00 0.12298 21400.00 21000.00 0.00 0.12393 21200.00 21200.00 0.00 0.12505 21400.00 21200.00 0.00 0.12546 21200.00 21400.00 0.00 0.12619 21400.00 21400.00 0.00 0.12621 21200.00 21400.00 5.00 0.12627 21200.00 21600.00 5.00 0.12631 21200.00 21400.00 10.00 0.12634 21200.00 21600.00 10.00 0.12640 21200.00 21600.00 15.00 0.12645 21200.00 21600.00 20.00 0.12647 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21241.77 21490.58 20.34 0.12672 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/420.ctf NX, NY, NZ: 512 512 1 MODE : real