CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/419.mrc Output diagnostic file name Micrographs_0601/419.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/419.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2524.000 7946.000 Mean, RMS : 4508.382 217.5063 TITLE 1: emi2mrc: 419.emi Mon Jan 06 14:10:55 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.05%, Obj 88.06%, Dif 73.67% TITLE 3: X 301.771, Y 844.602, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02251 10200.00 10000.00 0.00 0.02398 10400.00 10000.00 0.00 0.02554 10600.00 10000.00 0.00 0.02734 10800.00 10000.00 0.00 0.02911 11000.00 10000.00 0.00 0.03100 11200.00 10000.00 0.00 0.03301 11400.00 10000.00 0.00 0.03517 11600.00 10000.00 0.00 0.03748 11800.00 10000.00 0.00 0.03986 12000.00 10000.00 0.00 0.04262 12200.00 10000.00 0.00 0.04553 12400.00 10000.00 0.00 0.04856 12600.00 10000.00 0.00 0.05169 12800.00 10000.00 0.00 0.05507 13000.00 10000.00 0.00 0.05833 13200.00 10000.00 0.00 0.06155 13400.00 10000.00 0.00 0.06508 13600.00 10000.00 0.00 0.06867 13800.00 10000.00 0.00 0.07246 14000.00 10000.00 0.00 0.07621 14200.00 10000.00 0.00 0.07981 14400.00 10000.00 0.00 0.08264 14600.00 10000.00 0.00 0.08433 14800.00 10000.00 0.00 0.08505 14600.00 10200.00 0.00 0.08668 14800.00 10200.00 0.00 0.08733 14600.00 10400.00 0.00 0.08895 14800.00 10400.00 0.00 0.08954 14400.00 10600.00 0.00 0.08987 14600.00 10600.00 0.00 0.09148 14800.00 10600.00 0.00 0.09206 14400.00 10800.00 0.00 0.09251 14600.00 10800.00 0.00 0.09412 14800.00 10800.00 0.00 0.09464 14400.00 11000.00 0.00 0.09527 14600.00 11000.00 0.00 0.09681 14800.00 11000.00 0.00 0.09720 14400.00 11200.00 0.00 0.09790 14600.00 11200.00 0.00 0.09936 14800.00 11200.00 0.00 0.09968 14400.00 11400.00 0.00 0.10078 14600.00 11400.00 0.00 0.10224 14800.00 11400.00 0.00 0.10251 14400.00 11600.00 0.00 0.10373 14600.00 11600.00 0.00 0.10517 14800.00 11600.00 0.00 0.10534 14400.00 11800.00 0.00 0.10680 14600.00 11800.00 0.00 0.10816 14800.00 11800.00 0.00 0.10817 14400.00 12000.00 0.00 0.11008 14600.00 12000.00 0.00 0.11137 14400.00 12200.00 0.00 0.11368 14600.00 12200.00 0.00 0.11487 14400.00 12400.00 0.00 0.11746 14600.00 12400.00 0.00 0.11848 14400.00 12600.00 0.00 0.12099 14600.00 12600.00 0.00 0.12183 14200.00 12800.00 0.00 0.12204 14400.00 12800.00 0.00 0.12474 14600.00 12800.00 0.00 0.12539 14200.00 13000.00 0.00 0.12574 14400.00 13000.00 0.00 0.12836 14600.00 13000.00 0.00 0.12883 14200.00 13200.00 0.00 0.12966 14400.00 13200.00 0.00 0.13217 14600.00 13200.00 0.00 0.13238 14200.00 13400.00 0.00 0.13343 14400.00 13400.00 0.00 0.13581 14200.00 13600.00 0.00 0.13755 14400.00 13600.00 0.00 0.13971 14200.00 13800.00 0.00 0.14187 14400.00 13800.00 0.00 0.14365 14200.00 14000.00 0.00 0.14551 14400.00 14000.00 0.00 0.14682 14200.00 14200.00 0.00 0.14913 14400.00 14200.00 0.00 0.14955 14200.00 14400.00 0.00 0.15063 14200.00 14400.00 5.00 0.15069 14200.00 14400.00 10.00 0.15072 14200.00 14400.00 15.00 0.15074 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14261.37 14382.44 15.00 0.15094 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/419.ctf NX, NY, NZ: 512 512 1 MODE : real