CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/418.mrc Output diagnostic file name Micrographs_0601/418.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/418.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2428.000 8010.000 Mean, RMS : 4542.534 220.5056 TITLE 1: emi2mrc: 418.emi Mon Jan 06 14:10:01 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.05%, Obj 88.06%, Dif 73.67% TITLE 3: X 305.612, Y 843.876, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02157 10200.00 10000.00 0.00 0.02297 10400.00 10000.00 0.00 0.02420 10600.00 10000.00 0.00 0.02560 10800.00 10000.00 0.00 0.02696 11000.00 10000.00 0.00 0.02849 11200.00 10000.00 0.00 0.03017 11400.00 10000.00 0.00 0.03195 11600.00 10000.00 0.00 0.03400 11800.00 10000.00 0.00 0.03613 12000.00 10000.00 0.00 0.03832 12200.00 10000.00 0.00 0.04066 12400.00 10000.00 0.00 0.04330 12600.00 10000.00 0.00 0.04604 12800.00 10000.00 0.00 0.04905 13000.00 10000.00 0.00 0.05228 13200.00 10000.00 0.00 0.05549 13400.00 10000.00 0.00 0.05891 13600.00 10000.00 0.00 0.06265 13800.00 10000.00 0.00 0.06675 14000.00 10000.00 0.00 0.07091 14200.00 10000.00 0.00 0.07517 14400.00 10000.00 0.00 0.07933 14600.00 10000.00 0.00 0.08262 14800.00 10000.00 0.00 0.08425 15000.00 10000.00 0.00 0.08458 14600.00 10200.00 0.00 0.08491 14800.00 10200.00 0.00 0.08652 15000.00 10200.00 0.00 0.08677 14600.00 10400.00 0.00 0.08714 14800.00 10400.00 0.00 0.08871 15000.00 10400.00 0.00 0.08890 14600.00 10600.00 0.00 0.08958 14800.00 10600.00 0.00 0.09108 15000.00 10600.00 0.00 0.09120 14600.00 10800.00 0.00 0.09199 14800.00 10800.00 0.00 0.09344 15000.00 10800.00 0.00 0.09350 14600.00 11000.00 0.00 0.09465 14800.00 11000.00 0.00 0.09606 15000.00 11000.00 0.00 0.09609 14600.00 11200.00 0.00 0.09733 14800.00 11200.00 0.00 0.09870 14600.00 11400.00 0.00 0.10023 14800.00 11400.00 0.00 0.10154 14600.00 11600.00 0.00 0.10326 14800.00 11600.00 0.00 0.10455 14600.00 11800.00 0.00 0.10654 14800.00 11800.00 0.00 0.10778 14600.00 12000.00 0.00 0.10994 14800.00 12000.00 0.00 0.11113 14600.00 12200.00 0.00 0.11341 14800.00 12200.00 0.00 0.11455 14600.00 12400.00 0.00 0.11704 14800.00 12400.00 0.00 0.11813 14600.00 12600.00 0.00 0.12078 14800.00 12600.00 0.00 0.12177 14600.00 12800.00 0.00 0.12497 14800.00 12800.00 0.00 0.12578 14600.00 13000.00 0.00 0.12899 14800.00 13000.00 0.00 0.12963 14400.00 13200.00 0.00 0.13013 14600.00 13200.00 0.00 0.13335 14800.00 13200.00 0.00 0.13384 14400.00 13400.00 0.00 0.13462 14600.00 13400.00 0.00 0.13779 14800.00 13400.00 0.00 0.13808 14400.00 13600.00 0.00 0.13929 14600.00 13600.00 0.00 0.14232 14400.00 13800.00 0.00 0.14427 14600.00 13800.00 0.00 0.14703 14400.00 14000.00 0.00 0.14900 14600.00 14000.00 0.00 0.15141 14400.00 14200.00 0.00 0.15412 14600.00 14200.00 0.00 0.15594 14400.00 14400.00 0.00 0.15830 14600.00 14400.00 0.00 0.15950 14400.00 14600.00 0.00 0.16176 14600.00 14600.00 0.00 0.16195 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14479.52 14679.10 10.33 0.16282 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/418.ctf NX, NY, NZ: 512 512 1 MODE : real