CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/417.mrc Output diagnostic file name Micrographs_0601/417.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/417.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2552.000 8012.000 Mean, RMS : 4540.100 220.8186 TITLE 1: emi2mrc: 417.emi Mon Jan 06 14:09:17 2014 TITLE 2: kV 300, Mag 78000x, Def 23630, Spot 4, Int 55.05%, Obj 88.06%, Dif 73.67% TITLE 3: X 303.841, Y 845.753, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01795 10200.00 10000.00 0.00 0.01918 10400.00 10000.00 0.00 0.02038 10600.00 10000.00 0.00 0.02168 10800.00 10000.00 0.00 0.02288 11000.00 10000.00 0.00 0.02431 11200.00 10000.00 0.00 0.02597 11400.00 10000.00 0.00 0.02764 11600.00 10000.00 0.00 0.02949 11800.00 10000.00 0.00 0.03152 12000.00 10000.00 0.00 0.03367 12200.00 10000.00 0.00 0.03593 12400.00 10000.00 0.00 0.03850 12600.00 10000.00 0.00 0.04132 12800.00 10000.00 0.00 0.04438 13000.00 10000.00 0.00 0.04738 13200.00 10000.00 0.00 0.05026 13400.00 10000.00 0.00 0.05339 13600.00 10000.00 0.00 0.05648 13800.00 10000.00 0.00 0.05969 14000.00 10000.00 0.00 0.06325 14200.00 10000.00 0.00 0.06711 14400.00 10000.00 0.00 0.07101 14600.00 10000.00 0.00 0.07523 14800.00 10000.00 0.00 0.07949 15000.00 10000.00 0.00 0.08216 15200.00 10000.00 0.00 0.08297 15000.00 10200.00 0.00 0.08419 15200.00 10200.00 0.00 0.08492 15000.00 10400.00 0.00 0.08625 15200.00 10400.00 0.00 0.08693 15000.00 10600.00 0.00 0.08842 15200.00 10600.00 0.00 0.08910 15000.00 10800.00 0.00 0.09066 15200.00 10800.00 0.00 0.09137 15000.00 11000.00 0.00 0.09330 15200.00 11000.00 0.00 0.09400 15000.00 11200.00 0.00 0.09600 15200.00 11200.00 0.00 0.09669 15000.00 11400.00 0.00 0.09903 15200.00 11400.00 0.00 0.09969 15000.00 11600.00 0.00 0.10213 15200.00 11600.00 0.00 0.10274 14800.00 11800.00 0.00 0.10281 15000.00 11800.00 0.00 0.10531 15200.00 11800.00 0.00 0.10582 14800.00 12000.00 0.00 0.10607 15000.00 12000.00 0.00 0.10854 15200.00 12000.00 0.00 0.10900 14800.00 12200.00 0.00 0.10955 15000.00 12200.00 0.00 0.11199 15200.00 12200.00 0.00 0.11238 14800.00 12400.00 0.00 0.11337 15000.00 12400.00 0.00 0.11576 15200.00 12400.00 0.00 0.11607 14800.00 12600.00 0.00 0.11715 15000.00 12600.00 0.00 0.11953 15200.00 12600.00 0.00 0.11977 14800.00 12800.00 0.00 0.12123 15000.00 12800.00 0.00 0.12356 15200.00 12800.00 0.00 0.12366 14800.00 13000.00 0.00 0.12505 15000.00 13000.00 0.00 0.12729 14800.00 13200.00 0.00 0.12907 15000.00 13200.00 0.00 0.13121 14800.00 13400.00 0.00 0.13314 15000.00 13400.00 0.00 0.13516 14800.00 13600.00 0.00 0.13728 15000.00 13600.00 0.00 0.13914 14800.00 13800.00 0.00 0.14158 15000.00 13800.00 0.00 0.14325 14800.00 14000.00 0.00 0.14583 15000.00 14000.00 0.00 0.14725 14800.00 14200.00 0.00 0.15070 15000.00 14200.00 0.00 0.15165 14800.00 14400.00 0.00 0.15527 15000.00 14400.00 0.00 0.15563 14800.00 14600.00 0.00 0.16034 14600.00 14800.00 0.00 0.16087 14800.00 14800.00 0.00 0.16426 14800.00 15000.00 0.00 0.16463 14800.00 15000.00 5.00 0.16474 14800.00 15000.00 10.00 0.16481 14800.00 15000.00 15.00 0.16484 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14759.37 14954.87 17.17 0.16514 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/417.ctf NX, NY, NZ: 512 512 1 MODE : real