CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/416.mrc Output diagnostic file name Micrographs_0601/416.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/416.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2434.000 8752.000 Mean, RMS : 4518.011 219.6913 TITLE 1: emi2mrc: 416.emi Mon Jan 06 14:07:18 2014 TITLE 2: kV 300, Mag 78000x, Def 22810, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 300.337, Y 850.022, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02435 10200.00 10000.00 0.00 0.02626 10400.00 10000.00 0.00 0.02808 10600.00 10000.00 0.00 0.03021 10800.00 10000.00 0.00 0.03241 11000.00 10000.00 0.00 0.03497 11200.00 10000.00 0.00 0.03764 11400.00 10000.00 0.00 0.04015 11600.00 10000.00 0.00 0.04289 11800.00 10000.00 0.00 0.04595 12000.00 10000.00 0.00 0.04926 12200.00 10000.00 0.00 0.05256 12400.00 10000.00 0.00 0.05606 12600.00 10000.00 0.00 0.05954 12800.00 10000.00 0.00 0.06314 13000.00 10000.00 0.00 0.06685 13200.00 10000.00 0.00 0.07046 13400.00 10000.00 0.00 0.07439 13600.00 10000.00 0.00 0.07847 13800.00 10000.00 0.00 0.08269 14000.00 10000.00 0.00 0.08647 14200.00 10000.00 0.00 0.08870 14400.00 10000.00 0.00 0.08913 14200.00 10200.00 0.00 0.09115 14400.00 10200.00 0.00 0.09158 14000.00 10400.00 0.00 0.09170 14200.00 10400.00 0.00 0.09396 14400.00 10400.00 0.00 0.09436 14000.00 10600.00 0.00 0.09472 14200.00 10600.00 0.00 0.09697 14400.00 10600.00 0.00 0.09732 14000.00 10800.00 0.00 0.09783 14200.00 10800.00 0.00 0.10007 14400.00 10800.00 0.00 0.10034 14000.00 11000.00 0.00 0.10124 14200.00 11000.00 0.00 0.10343 14400.00 11000.00 0.00 0.10362 14000.00 11200.00 0.00 0.10458 14200.00 11200.00 0.00 0.10669 14400.00 11200.00 0.00 0.10677 14000.00 11400.00 0.00 0.10810 14200.00 11400.00 0.00 0.11012 14000.00 11600.00 0.00 0.11156 14200.00 11600.00 0.00 0.11355 14000.00 11800.00 0.00 0.11545 14200.00 11800.00 0.00 0.11747 14000.00 12000.00 0.00 0.11955 14200.00 12000.00 0.00 0.12154 14000.00 12200.00 0.00 0.12367 14200.00 12200.00 0.00 0.12555 14000.00 12400.00 0.00 0.12817 14200.00 12400.00 0.00 0.12985 14000.00 12600.00 0.00 0.13238 14200.00 12600.00 0.00 0.13383 14000.00 12800.00 0.00 0.13676 14200.00 12800.00 0.00 0.13797 14000.00 13000.00 0.00 0.14104 14200.00 13000.00 0.00 0.14201 13800.00 13200.00 0.00 0.14207 14000.00 13200.00 0.00 0.14572 14200.00 13200.00 0.00 0.14632 13800.00 13400.00 0.00 0.14676 14000.00 13400.00 0.00 0.15015 14200.00 13400.00 0.00 0.15030 13800.00 13600.00 0.00 0.15139 14000.00 13600.00 0.00 0.15440 13800.00 13800.00 0.00 0.15632 14000.00 13800.00 0.00 0.15846 13800.00 14000.00 0.00 0.15928 14000.00 14000.00 0.00 0.16051 14000.00 14200.00 0.00 0.16084 14000.00 14200.00 5.00 0.16086 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13926.58 14143.84 11.77 0.16126 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/416.ctf NX, NY, NZ: 512 512 1 MODE : real