CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/415.mrc Output diagnostic file name Micrographs_0601/415.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/415.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2428.000 8518.000 Mean, RMS : 4517.549 220.7971 TITLE 1: emi2mrc: 415.emi Mon Jan 06 14:06:24 2014 TITLE 2: kV 300, Mag 78000x, Def 22810, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 298.317, Y 851.651, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01577 10200.00 10000.00 0.00 0.01668 10400.00 10000.00 0.00 0.01767 10600.00 10000.00 0.00 0.01909 10800.00 10000.00 0.00 0.02063 11000.00 10000.00 0.00 0.02223 11200.00 10000.00 0.00 0.02395 11400.00 10000.00 0.00 0.02582 11600.00 10000.00 0.00 0.02778 11800.00 10000.00 0.00 0.02969 12000.00 10000.00 0.00 0.03170 12200.00 10000.00 0.00 0.03401 12400.00 10000.00 0.00 0.03660 12600.00 10000.00 0.00 0.03930 12800.00 10000.00 0.00 0.04220 13000.00 10000.00 0.00 0.04501 13200.00 10000.00 0.00 0.04777 13400.00 10000.00 0.00 0.05066 13600.00 10000.00 0.00 0.05352 13800.00 10000.00 0.00 0.05660 14000.00 10000.00 0.00 0.05980 14200.00 10000.00 0.00 0.06332 14400.00 10000.00 0.00 0.06696 14600.00 10000.00 0.00 0.07049 14800.00 10000.00 0.00 0.07414 15000.00 10000.00 0.00 0.07731 15200.00 10000.00 0.00 0.07912 15400.00 10000.00 0.00 0.07966 15600.00 10000.00 0.00 0.07982 15200.00 10200.00 0.00 0.08119 15400.00 10200.00 0.00 0.08170 15600.00 10200.00 0.00 0.08182 15200.00 10400.00 0.00 0.08322 15400.00 10400.00 0.00 0.08370 15600.00 10400.00 0.00 0.08378 15200.00 10600.00 0.00 0.08531 15400.00 10600.00 0.00 0.08575 15600.00 10600.00 0.00 0.08576 15200.00 10800.00 0.00 0.08746 15400.00 10800.00 0.00 0.08784 15000.00 11000.00 0.00 0.08808 15200.00 11000.00 0.00 0.08980 15400.00 11000.00 0.00 0.09013 15000.00 11200.00 0.00 0.09044 15200.00 11200.00 0.00 0.09217 15400.00 11200.00 0.00 0.09245 15000.00 11400.00 0.00 0.09311 15200.00 11400.00 0.00 0.09481 15400.00 11400.00 0.00 0.09503 15000.00 11600.00 0.00 0.09595 15200.00 11600.00 0.00 0.09763 15400.00 11600.00 0.00 0.09777 15000.00 11800.00 0.00 0.09885 15200.00 11800.00 0.00 0.10050 15400.00 11800.00 0.00 0.10055 15000.00 12000.00 0.00 0.10183 15200.00 12000.00 0.00 0.10345 15000.00 12200.00 0.00 0.10507 15200.00 12200.00 0.00 0.10663 15000.00 12400.00 0.00 0.10850 15200.00 12400.00 0.00 0.10998 15000.00 12600.00 0.00 0.11202 15200.00 12600.00 0.00 0.11342 15000.00 12800.00 0.00 0.11585 15200.00 12800.00 0.00 0.11711 15000.00 13000.00 0.00 0.11955 15200.00 13000.00 0.00 0.12067 15000.00 13200.00 0.00 0.12349 15200.00 13200.00 0.00 0.12452 15000.00 13400.00 0.00 0.12735 15200.00 13400.00 0.00 0.12827 15000.00 13600.00 0.00 0.13127 15200.00 13600.00 0.00 0.13198 14800.00 13800.00 0.00 0.13224 15000.00 13800.00 0.00 0.13532 15200.00 13800.00 0.00 0.13579 14800.00 14000.00 0.00 0.13638 15000.00 14000.00 0.00 0.13939 15200.00 14000.00 0.00 0.13959 14800.00 14200.00 0.00 0.14109 15000.00 14200.00 0.00 0.14391 14800.00 14400.00 0.00 0.14548 15000.00 14400.00 0.00 0.14800 14800.00 14600.00 0.00 0.15018 15000.00 14600.00 0.00 0.15216 14800.00 14800.00 0.00 0.15443 15000.00 14800.00 0.00 0.15557 14800.00 15000.00 0.00 0.15705 15000.00 15000.00 0.00 0.15733 14800.00 15000.00 15.00 0.15734 14800.00 15000.00 20.00 0.15738 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14880.07 15055.04 13.36 0.15786 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/415.ctf NX, NY, NZ: 512 512 1 MODE : real