CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/414.mrc Output diagnostic file name Micrographs_0601/414.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/414.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2450.000 8136.000 Mean, RMS : 4509.282 217.9174 TITLE 1: emi2mrc: 414.emi Mon Jan 06 14:05:24 2014 TITLE 2: kV 300, Mag 78000x, Def 22810, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 296.533, Y 853.728, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01147 10200.00 10000.00 0.00 0.01250 10400.00 10000.00 0.00 0.01356 10600.00 10000.00 0.00 0.01467 10800.00 10000.00 0.00 0.01580 11000.00 10000.00 0.00 0.01707 11200.00 10000.00 0.00 0.01824 11400.00 10000.00 0.00 0.01942 11600.00 10000.00 0.00 0.02100 11800.00 10000.00 0.00 0.02280 12000.00 10000.00 0.00 0.02468 12200.00 10000.00 0.00 0.02665 12400.00 10000.00 0.00 0.02893 12600.00 10000.00 0.00 0.03121 12800.00 10000.00 0.00 0.03338 13000.00 10000.00 0.00 0.03571 13200.00 10000.00 0.00 0.03819 13400.00 10000.00 0.00 0.04079 13600.00 10000.00 0.00 0.04352 13800.00 10000.00 0.00 0.04642 14000.00 10000.00 0.00 0.04914 14200.00 10000.00 0.00 0.05193 14400.00 10000.00 0.00 0.05498 14600.00 10000.00 0.00 0.05826 14800.00 10000.00 0.00 0.06194 15000.00 10000.00 0.00 0.06575 15200.00 10000.00 0.00 0.06972 15400.00 10000.00 0.00 0.07302 15600.00 10000.00 0.00 0.07466 15800.00 10000.00 0.00 0.07526 16000.00 10000.00 0.00 0.07562 15600.00 10200.00 0.00 0.07634 15800.00 10200.00 0.00 0.07692 16000.00 10200.00 0.00 0.07722 15600.00 10400.00 0.00 0.07811 15800.00 10400.00 0.00 0.07866 16000.00 10400.00 0.00 0.07889 15600.00 10600.00 0.00 0.08013 15800.00 10600.00 0.00 0.08065 16000.00 10600.00 0.00 0.08083 15600.00 10800.00 0.00 0.08213 15800.00 10800.00 0.00 0.08261 16000.00 10800.00 0.00 0.08273 15600.00 11000.00 0.00 0.08434 15800.00 11000.00 0.00 0.08481 16000.00 11000.00 0.00 0.08490 15400.00 11200.00 0.00 0.08505 15600.00 11200.00 0.00 0.08669 15800.00 11200.00 0.00 0.08714 16000.00 11200.00 0.00 0.08717 15400.00 11400.00 0.00 0.08747 15600.00 11400.00 0.00 0.08909 15800.00 11400.00 0.00 0.08948 15400.00 11600.00 0.00 0.08992 15600.00 11600.00 0.00 0.09151 15800.00 11600.00 0.00 0.09186 15400.00 11800.00 0.00 0.09251 15600.00 11800.00 0.00 0.09412 15800.00 11800.00 0.00 0.09444 15400.00 12000.00 0.00 0.09512 15600.00 12000.00 0.00 0.09678 15800.00 12000.00 0.00 0.09707 15400.00 12200.00 0.00 0.09804 15600.00 12200.00 0.00 0.09973 15800.00 12200.00 0.00 0.09989 15400.00 12400.00 0.00 0.10118 15600.00 12400.00 0.00 0.10278 15400.00 12600.00 0.00 0.10408 15600.00 12600.00 0.00 0.10554 15400.00 12800.00 0.00 0.10713 15600.00 12800.00 0.00 0.10846 15400.00 13000.00 0.00 0.11006 15600.00 13000.00 0.00 0.11131 15400.00 13200.00 0.00 0.11323 15600.00 13200.00 0.00 0.11443 15400.00 13400.00 0.00 0.11638 15600.00 13400.00 0.00 0.11751 15400.00 13600.00 0.00 0.11963 15600.00 13600.00 0.00 0.12067 15400.00 13800.00 0.00 0.12316 15600.00 13800.00 0.00 0.12404 15400.00 14000.00 0.00 0.12646 15600.00 14000.00 0.00 0.12714 15400.00 14200.00 0.00 0.12986 15600.00 14200.00 0.00 0.13035 15400.00 14400.00 0.00 0.13317 15600.00 14400.00 0.00 0.13341 15200.00 14600.00 0.00 0.13363 15400.00 14600.00 0.00 0.13674 15200.00 14800.00 0.00 0.13706 15400.00 14800.00 0.00 0.13995 15200.00 15000.00 0.00 0.14043 15400.00 15000.00 0.00 0.14295 15200.00 15200.00 0.00 0.14388 15400.00 15200.00 0.00 0.14562 15200.00 15400.00 0.00 0.14628 15400.00 15400.00 0.00 0.14734 15200.00 15600.00 0.00 0.14738 15400.00 15600.00 0.00 0.14750 15200.00 15600.00 5.00 0.14757 15200.00 15600.00 10.00 0.14770 15200.00 15600.00 15.00 0.14776 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15298.61 15542.24 12.77 0.14820 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/414.ctf NX, NY, NZ: 512 512 1 MODE : real