CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/413.mrc Output diagnostic file name Micrographs_0601/413.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/413.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2234.000 8794.000 Mean, RMS : 4521.548 221.1235 TITLE 1: emi2mrc: 413.emi Mon Jan 06 14:04:34 2014 TITLE 2: kV 300, Mag 78000x, Def 23460, Spot 4, Int 55.05%, Obj 88.07%, Dif 73.67% TITLE 3: X 294.770, Y 855.955, Z -24.311, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00253 10200.00 10000.00 0.00 0.00277 10400.00 10000.00 0.00 0.00318 10600.00 10000.00 0.00 0.00358 10800.00 10000.00 0.00 0.00392 11000.00 10000.00 0.00 0.00445 11200.00 10000.00 0.00 0.00508 11400.00 10000.00 0.00 0.00582 11600.00 10000.00 0.00 0.00684 11800.00 10000.00 0.00 0.00781 12000.00 10000.00 0.00 0.00884 12200.00 10000.00 0.00 0.01010 12400.00 10000.00 0.00 0.01130 12600.00 10000.00 0.00 0.01254 12800.00 10000.00 0.00 0.01400 13000.00 10000.00 0.00 0.01549 13200.00 10000.00 0.00 0.01704 13400.00 10000.00 0.00 0.01875 13600.00 10000.00 0.00 0.02048 13800.00 10000.00 0.00 0.02225 14000.00 10000.00 0.00 0.02414 14200.00 10000.00 0.00 0.02618 14400.00 10000.00 0.00 0.02818 14600.00 10000.00 0.00 0.03028 14800.00 10000.00 0.00 0.03264 15000.00 10000.00 0.00 0.03505 15200.00 10000.00 0.00 0.03739 15400.00 10000.00 0.00 0.03975 15600.00 10000.00 0.00 0.04230 15800.00 10000.00 0.00 0.04500 16000.00 10000.00 0.00 0.04792 16200.00 10000.00 0.00 0.05085 16400.00 10000.00 0.00 0.05398 16600.00 10000.00 0.00 0.05746 16800.00 10000.00 0.00 0.06093 17000.00 10000.00 0.00 0.06385 17200.00 10000.00 0.00 0.06523 17400.00 10000.00 0.00 0.06568 17600.00 10000.00 0.00 0.06576 17200.00 10200.00 0.00 0.06648 17400.00 10200.00 0.00 0.06689 17600.00 10200.00 0.00 0.06694 17200.00 10400.00 0.00 0.06763 17400.00 10400.00 0.00 0.06803 17600.00 10400.00 0.00 0.06807 17200.00 10600.00 0.00 0.06906 17400.00 10600.00 0.00 0.06946 17600.00 10600.00 0.00 0.06949 17200.00 10800.00 0.00 0.07046 17400.00 10800.00 0.00 0.07085 17200.00 11000.00 0.00 0.07183 17400.00 11000.00 0.00 0.07224 17200.00 11200.00 0.00 0.07339 17400.00 11200.00 0.00 0.07379 17200.00 11400.00 0.00 0.07513 17400.00 11400.00 0.00 0.07547 17200.00 11600.00 0.00 0.07671 17400.00 11600.00 0.00 0.07697 17200.00 11800.00 0.00 0.07825 17400.00 11800.00 0.00 0.07843 17000.00 12000.00 0.00 0.07862 17200.00 12000.00 0.00 0.07992 17400.00 12000.00 0.00 0.08009 17000.00 12200.00 0.00 0.08048 17200.00 12200.00 0.00 0.08178 17400.00 12200.00 0.00 0.08198 17000.00 12400.00 0.00 0.08251 17200.00 12400.00 0.00 0.08384 17400.00 12400.00 0.00 0.08405 17000.00 12600.00 0.00 0.08460 17200.00 12600.00 0.00 0.08595 17400.00 12600.00 0.00 0.08611 17000.00 12800.00 0.00 0.08687 17200.00 12800.00 0.00 0.08816 17400.00 12800.00 0.00 0.08818 17000.00 13000.00 0.00 0.08911 17200.00 13000.00 0.00 0.09029 17000.00 13200.00 0.00 0.09154 17200.00 13200.00 0.00 0.09263 17000.00 13400.00 0.00 0.09392 17200.00 13400.00 0.00 0.09497 17000.00 13600.00 0.00 0.09640 17200.00 13600.00 0.00 0.09740 17000.00 13800.00 0.00 0.09902 17200.00 13800.00 0.00 0.09997 17000.00 14000.00 0.00 0.10162 17200.00 14000.00 0.00 0.10252 17000.00 14200.00 0.00 0.10451 17200.00 14200.00 0.00 0.10537 17000.00 14400.00 0.00 0.10746 17200.00 14400.00 0.00 0.10834 17000.00 14600.00 0.00 0.11087 17200.00 14600.00 0.00 0.11176 17000.00 14800.00 0.00 0.11438 17200.00 14800.00 0.00 0.11520 17000.00 15000.00 0.00 0.11799 17200.00 15000.00 0.00 0.11867 17000.00 15200.00 0.00 0.12167 17200.00 15200.00 0.00 0.12218 17000.00 15400.00 0.00 0.12529 17200.00 15400.00 0.00 0.12564 16800.00 15600.00 0.00 0.12606 17000.00 15600.00 0.00 0.12915 17200.00 15600.00 0.00 0.12926 16800.00 15800.00 0.00 0.12982 17000.00 15800.00 0.00 0.13281 16800.00 16000.00 0.00 0.13378 17000.00 16000.00 0.00 0.13667 16800.00 16200.00 0.00 0.13757 17000.00 16200.00 0.00 0.14034 16800.00 16400.00 0.00 0.14171 17000.00 16400.00 0.00 0.14422 16800.00 16600.00 0.00 0.14567 17000.00 16600.00 0.00 0.14783 16800.00 16800.00 0.00 0.14944 17000.00 16800.00 0.00 0.15104 16800.00 17000.00 0.00 0.15265 17000.00 17000.00 0.00 0.15300 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16892.32 17042.28 14.01 0.15365 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/413.ctf NX, NY, NZ: 512 512 1 MODE : real