CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/411.mrc Output diagnostic file name Micrographs_0601/411.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/411.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2566.000 7886.000 Mean, RMS : 4477.568 221.4477 TITLE 1: emi2mrc: 411.emi Mon Jan 06 14:01:08 2014 TITLE 2: kV 300, Mag 78000x, Def 25430, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 289.310, Y 861.448, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01758 13000.00 10000.00 0.00 -0.01721 13200.00 10000.00 0.00 -0.01690 13400.00 10000.00 0.00 -0.01664 13600.00 10000.00 0.00 -0.01628 13800.00 10000.00 0.00 -0.01587 14000.00 10000.00 0.00 -0.01552 14200.00 10000.00 0.00 -0.01511 14400.00 10000.00 0.00 -0.01466 14600.00 10000.00 0.00 -0.01428 14800.00 10000.00 0.00 -0.01381 15000.00 10000.00 0.00 -0.01327 15200.00 10000.00 0.00 -0.01287 15400.00 10000.00 0.00 -0.01238 15600.00 10000.00 0.00 -0.01168 15800.00 10000.00 0.00 -0.01093 16000.00 10000.00 0.00 -0.01006 16200.00 10000.00 0.00 -0.00914 16400.00 10000.00 0.00 -0.00828 16600.00 10000.00 0.00 -0.00739 16800.00 10000.00 0.00 -0.00652 17000.00 10000.00 0.00 -0.00572 17200.00 10000.00 0.00 -0.00487 17400.00 10000.00 0.00 -0.00391 17600.00 10000.00 0.00 -0.00283 17800.00 10000.00 0.00 -0.00170 18000.00 10000.00 0.00 -0.00072 18200.00 10000.00 0.00 0.00024 18400.00 10000.00 0.00 0.00123 18600.00 10000.00 0.00 0.00218 18800.00 10000.00 0.00 0.00315 19000.00 10000.00 0.00 0.00402 19200.00 10000.00 0.00 0.00498 19400.00 10000.00 0.00 0.00604 19600.00 10000.00 0.00 0.00690 19800.00 10000.00 0.00 0.00786 20000.00 10000.00 0.00 0.00899 20200.00 10000.00 0.00 0.01003 20400.00 10000.00 0.00 0.01111 20600.00 10000.00 0.00 0.01234 20800.00 10000.00 0.00 0.01370 21000.00 10000.00 0.00 0.01514 21200.00 10000.00 0.00 0.01652 21400.00 10000.00 0.00 0.01804 21600.00 10000.00 0.00 0.01972 21800.00 10000.00 0.00 0.02135 22000.00 10000.00 0.00 0.02316 22200.00 10000.00 0.00 0.02501 22400.00 10000.00 0.00 0.02695 22600.00 10000.00 0.00 0.02903 22800.00 10000.00 0.00 0.03106 23000.00 10000.00 0.00 0.03271 23200.00 10000.00 0.00 0.03359 23400.00 10000.00 0.00 0.03405 23600.00 10000.00 0.00 0.03435 23800.00 10000.00 0.00 0.03443 23400.00 10200.00 0.00 0.03446 23600.00 10200.00 0.00 0.03483 23800.00 10200.00 0.00 0.03491 23400.00 10400.00 0.00 0.03500 23600.00 10400.00 0.00 0.03539 23800.00 10400.00 0.00 0.03540 23400.00 10600.00 0.00 0.03552 23600.00 10600.00 0.00 0.03584 23600.00 10800.00 0.00 0.03607 23600.00 11000.00 0.00 0.03619 23400.00 11200.00 0.00 0.03625 23600.00 11200.00 0.00 0.03645 23400.00 11400.00 0.00 0.03670 23600.00 11400.00 0.00 0.03687 23400.00 11600.00 0.00 0.03712 23600.00 11600.00 0.00 0.03733 23400.00 11800.00 0.00 0.03775 23600.00 11800.00 0.00 0.03798 23400.00 12000.00 0.00 0.03834 23600.00 12000.00 0.00 0.03855 23400.00 12200.00 0.00 0.03894 23600.00 12200.00 0.00 0.03915 23200.00 12400.00 0.00 0.03920 23400.00 12400.00 0.00 0.03980 23600.00 12400.00 0.00 0.03999 23200.00 12600.00 0.00 0.04005 23400.00 12600.00 0.00 0.04064 23600.00 12600.00 0.00 0.04081 23200.00 12800.00 0.00 0.04085 23400.00 12800.00 0.00 0.04143 23600.00 12800.00 0.00 0.04159 23200.00 13000.00 0.00 0.04161 23400.00 13000.00 0.00 0.04220 23600.00 13000.00 0.00 0.04234 23200.00 13200.00 0.00 0.04242 23400.00 13200.00 0.00 0.04300 23600.00 13200.00 0.00 0.04311 23200.00 13400.00 0.00 0.04321 23400.00 13400.00 0.00 0.04374 23600.00 13400.00 0.00 0.04383 23200.00 13600.00 0.00 0.04407 23400.00 13600.00 0.00 0.04453 23600.00 13600.00 0.00 0.04463 23200.00 13800.00 0.00 0.04489 23400.00 13800.00 0.00 0.04532 23600.00 13800.00 0.00 0.04545 23200.00 14000.00 0.00 0.04571 23400.00 14000.00 0.00 0.04616 23600.00 14000.00 0.00 0.04633 23200.00 14200.00 0.00 0.04675 23400.00 14200.00 0.00 0.04723 23600.00 14200.00 0.00 0.04740 23200.00 14400.00 0.00 0.04774 23400.00 14400.00 0.00 0.04823 23600.00 14400.00 0.00 0.04836 23200.00 14600.00 0.00 0.04887 23400.00 14600.00 0.00 0.04936 23600.00 14600.00 0.00 0.04944 23200.00 14800.00 0.00 0.05014 23400.00 14800.00 0.00 0.05060 23200.00 15000.00 0.00 0.05138 23400.00 15000.00 0.00 0.05176 23200.00 15200.00 0.00 0.05251 23400.00 15200.00 0.00 0.05284 23200.00 15400.00 0.00 0.05369 23400.00 15400.00 0.00 0.05402 23200.00 15600.00 0.00 0.05494 23400.00 15600.00 0.00 0.05524 23200.00 15800.00 0.00 0.05603 23400.00 15800.00 0.00 0.05627 23000.00 16000.00 0.00 0.05634 23200.00 16000.00 0.00 0.05722 23400.00 16000.00 0.00 0.05745 23000.00 16200.00 0.00 0.05756 23200.00 16200.00 0.00 0.05842 23400.00 16200.00 0.00 0.05864 23000.00 16400.00 0.00 0.05883 23200.00 16400.00 0.00 0.05965 23400.00 16400.00 0.00 0.05987 23000.00 16600.00 0.00 0.06003 23200.00 16600.00 0.00 0.06084 23400.00 16600.00 0.00 0.06108 23000.00 16800.00 0.00 0.06122 23200.00 16800.00 0.00 0.06209 23400.00 16800.00 0.00 0.06240 23000.00 17000.00 0.00 0.06275 23200.00 17000.00 0.00 0.06369 23400.00 17000.00 0.00 0.06404 23000.00 17200.00 0.00 0.06433 23200.00 17200.00 0.00 0.06529 23400.00 17200.00 0.00 0.06560 23000.00 17400.00 0.00 0.06584 23200.00 17400.00 0.00 0.06678 23400.00 17400.00 0.00 0.06702 23000.00 17600.00 0.00 0.06728 23200.00 17600.00 0.00 0.06818 23400.00 17600.00 0.00 0.06836 23000.00 17800.00 0.00 0.06883 23200.00 17800.00 0.00 0.06968 23400.00 17800.00 0.00 0.06982 23000.00 18000.00 0.00 0.07038 23200.00 18000.00 0.00 0.07121 23400.00 18000.00 0.00 0.07134 23000.00 18200.00 0.00 0.07188 23200.00 18200.00 0.00 0.07272 23400.00 18200.00 0.00 0.07284 23000.00 18400.00 0.00 0.07349 23200.00 18400.00 0.00 0.07434 23400.00 18400.00 0.00 0.07445 23000.00 18600.00 0.00 0.07522 23200.00 18600.00 0.00 0.07604 23400.00 18600.00 0.00 0.07612 23000.00 18800.00 0.00 0.07698 23200.00 18800.00 0.00 0.07778 23400.00 18800.00 0.00 0.07779 23000.00 19000.00 0.00 0.07865 23200.00 19000.00 0.00 0.07938 23000.00 19200.00 0.00 0.08038 23200.00 19200.00 0.00 0.08105 23000.00 19400.00 0.00 0.08221 23200.00 19400.00 0.00 0.08285 23000.00 19600.00 0.00 0.08418 23200.00 19600.00 0.00 0.08479 23000.00 19800.00 0.00 0.08609 23200.00 19800.00 0.00 0.08665 23000.00 20000.00 0.00 0.08789 23200.00 20000.00 0.00 0.08842 23000.00 20200.00 0.00 0.08983 23200.00 20200.00 0.00 0.09036 23000.00 20400.00 0.00 0.09187 23200.00 20400.00 0.00 0.09240 23000.00 20600.00 0.00 0.09405 23200.00 20600.00 0.00 0.09456 23000.00 20800.00 0.00 0.09636 23200.00 20800.00 0.00 0.09681 23000.00 21000.00 0.00 0.09894 23200.00 21000.00 0.00 0.09928 23000.00 21200.00 0.00 0.10142 23200.00 21200.00 0.00 0.10163 22800.00 21400.00 0.00 0.10174 23000.00 21400.00 0.00 0.10393 23200.00 21400.00 0.00 0.10403 22800.00 21600.00 0.00 0.10452 23000.00 21600.00 0.00 0.10669 22800.00 21800.00 0.00 0.10739 23000.00 21800.00 0.00 0.10951 22800.00 22000.00 0.00 0.11041 23000.00 22000.00 0.00 0.11241 22800.00 22200.00 0.00 0.11347 23000.00 22200.00 0.00 0.11531 22800.00 22400.00 0.00 0.11673 23000.00 22400.00 0.00 0.11840 22800.00 22600.00 0.00 0.11984 23000.00 22600.00 0.00 0.12129 22800.00 22800.00 0.00 0.12319 23000.00 22800.00 0.00 0.12407 22800.00 23000.00 0.00 0.12515 22800.00 23000.00 5.00 0.12531 22800.00 23000.00 10.00 0.12545 22800.00 23000.00 15.00 0.12555 22800.00 23000.00 20.00 0.12563 22800.00 23000.00 25.00 0.12567 22800.00 23000.00 30.00 0.12568 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22857.83 23013.69 26.51 0.12584 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/411.ctf NX, NY, NZ: 512 512 1 MODE : real