CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/410.mrc Output diagnostic file name Micrographs_0601/410.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/410.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2292.000 7752.000 Mean, RMS : 4475.628 223.5211 TITLE 1: emi2mrc: 410.emi Mon Jan 06 14:00:12 2014 TITLE 2: kV 300, Mag 78000x, Def 25430, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 291.015, Y 863.836, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02755 15200.00 10000.00 0.00 -0.02731 15400.00 10000.00 0.00 -0.02694 15600.00 10000.00 0.00 -0.02651 15800.00 10000.00 0.00 -0.02613 16000.00 10000.00 0.00 -0.02567 16200.00 10000.00 0.00 -0.02504 16400.00 10000.00 0.00 -0.02440 16600.00 10000.00 0.00 -0.02399 16800.00 10000.00 0.00 -0.02363 17000.00 10000.00 0.00 -0.02306 17200.00 10000.00 0.00 -0.02238 17400.00 10000.00 0.00 -0.02177 17600.00 10000.00 0.00 -0.02112 17800.00 10000.00 0.00 -0.02056 18000.00 10000.00 0.00 -0.02002 18200.00 10000.00 0.00 -0.01935 18400.00 10000.00 0.00 -0.01867 18600.00 10000.00 0.00 -0.01790 18800.00 10000.00 0.00 -0.01709 19000.00 10000.00 0.00 -0.01621 19200.00 10000.00 0.00 -0.01519 19400.00 10000.00 0.00 -0.01420 19600.00 10000.00 0.00 -0.01333 19800.00 10000.00 0.00 -0.01250 20000.00 10000.00 0.00 -0.01159 20200.00 10000.00 0.00 -0.01068 20400.00 10000.00 0.00 -0.00993 20600.00 10000.00 0.00 -0.00924 20800.00 10000.00 0.00 -0.00846 21000.00 10000.00 0.00 -0.00766 21200.00 10000.00 0.00 -0.00701 21400.00 10000.00 0.00 -0.00635 21600.00 10000.00 0.00 -0.00546 21800.00 10000.00 0.00 -0.00462 22000.00 10000.00 0.00 -0.00388 22200.00 10000.00 0.00 -0.00314 22400.00 10000.00 0.00 -0.00233 22600.00 10000.00 0.00 -0.00138 22800.00 10000.00 0.00 -0.00025 23000.00 10000.00 0.00 0.00100 23200.00 10000.00 0.00 0.00217 23400.00 10000.00 0.00 0.00338 23600.00 10000.00 0.00 0.00480 23800.00 10000.00 0.00 0.00628 24000.00 10000.00 0.00 0.00772 24200.00 10000.00 0.00 0.00920 24400.00 10000.00 0.00 0.01084 24600.00 10000.00 0.00 0.01258 24800.00 10000.00 0.00 0.01433 25000.00 10000.00 0.00 0.01608 25200.00 10000.00 0.00 0.01785 25400.00 10000.00 0.00 0.01955 25600.00 10000.00 0.00 0.02075 25800.00 10000.00 0.00 0.02136 26000.00 10000.00 0.00 0.02169 26200.00 10000.00 0.00 0.02191 26400.00 10000.00 0.00 0.02206 26600.00 10000.00 0.00 0.02214 26200.00 10200.00 0.00 0.02238 26400.00 10200.00 0.00 0.02256 26600.00 10200.00 0.00 0.02264 26200.00 10400.00 0.00 0.02293 26400.00 10400.00 0.00 0.02313 26600.00 10400.00 0.00 0.02319 26200.00 10600.00 0.00 0.02342 26400.00 10600.00 0.00 0.02359 26600.00 10600.00 0.00 0.02359 26000.00 10800.00 0.00 0.02360 26200.00 10800.00 0.00 0.02385 26400.00 10800.00 0.00 0.02399 26000.00 11000.00 0.00 0.02411 26200.00 11000.00 0.00 0.02435 26400.00 11000.00 0.00 0.02447 26000.00 11200.00 0.00 0.02463 26200.00 11200.00 0.00 0.02482 26400.00 11200.00 0.00 0.02491 26000.00 11400.00 0.00 0.02506 26200.00 11400.00 0.00 0.02523 26400.00 11400.00 0.00 0.02532 26000.00 11600.00 0.00 0.02553 26200.00 11600.00 0.00 0.02571 26400.00 11600.00 0.00 0.02578 25800.00 11800.00 0.00 0.02587 26000.00 11800.00 0.00 0.02613 26200.00 11800.00 0.00 0.02627 26400.00 11800.00 0.00 0.02630 25800.00 12000.00 0.00 0.02647 26000.00 12000.00 0.00 0.02667 26200.00 12000.00 0.00 0.02676 25800.00 12200.00 0.00 0.02697 26000.00 12200.00 0.00 0.02711 26200.00 12200.00 0.00 0.02717 25800.00 12400.00 0.00 0.02730 26000.00 12400.00 0.00 0.02742 26200.00 12400.00 0.00 0.02747 25800.00 12600.00 0.00 0.02754 26000.00 12600.00 0.00 0.02766 26200.00 12600.00 0.00 0.02773 26400.00 12600.00 0.00 0.02774 25800.00 12800.00 0.00 0.02795 26000.00 12800.00 0.00 0.02809 26200.00 12800.00 0.00 0.02820 26400.00 12800.00 0.00 0.02824 25800.00 13000.00 0.00 0.02844 26000.00 13000.00 0.00 0.02862 26200.00 13000.00 0.00 0.02878 26400.00 13000.00 0.00 0.02883 25800.00 13200.00 0.00 0.02903 26000.00 13200.00 0.00 0.02924 26200.00 13200.00 0.00 0.02939 26400.00 13200.00 0.00 0.02940 25800.00 13400.00 0.00 0.02964 26000.00 13400.00 0.00 0.02984 26200.00 13400.00 0.00 0.02995 25800.00 13600.00 0.00 0.03018 26000.00 13600.00 0.00 0.03037 26200.00 13600.00 0.00 0.03046 25800.00 13800.00 0.00 0.03077 26000.00 13800.00 0.00 0.03096 26200.00 13800.00 0.00 0.03106 26400.00 13800.00 0.00 0.03107 25800.00 14000.00 0.00 0.03146 26000.00 14000.00 0.00 0.03166 26200.00 14000.00 0.00 0.03178 26400.00 14000.00 0.00 0.03182 25800.00 14200.00 0.00 0.03224 26000.00 14200.00 0.00 0.03244 26200.00 14200.00 0.00 0.03255 26400.00 14200.00 0.00 0.03258 25800.00 14400.00 0.00 0.03304 26000.00 14400.00 0.00 0.03319 26200.00 14400.00 0.00 0.03328 25600.00 14600.00 0.00 0.03330 25800.00 14600.00 0.00 0.03381 26000.00 14600.00 0.00 0.03390 26200.00 14600.00 0.00 0.03398 25800.00 14800.00 0.00 0.03442 26000.00 14800.00 0.00 0.03451 26200.00 14800.00 0.00 0.03460 25600.00 15000.00 0.00 0.03461 25800.00 15000.00 0.00 0.03506 26000.00 15000.00 0.00 0.03517 26200.00 15000.00 0.00 0.03526 25600.00 15200.00 0.00 0.03533 25800.00 15200.00 0.00 0.03576 26000.00 15200.00 0.00 0.03587 26200.00 15200.00 0.00 0.03594 25600.00 15400.00 0.00 0.03601 25800.00 15400.00 0.00 0.03646 26000.00 15400.00 0.00 0.03658 26200.00 15400.00 0.00 0.03665 25600.00 15600.00 0.00 0.03685 25800.00 15600.00 0.00 0.03733 26000.00 15600.00 0.00 0.03745 26200.00 15600.00 0.00 0.03749 25600.00 15800.00 0.00 0.03775 25800.00 15800.00 0.00 0.03820 26000.00 15800.00 0.00 0.03826 25600.00 16000.00 0.00 0.03864 25800.00 16000.00 0.00 0.03904 26000.00 16000.00 0.00 0.03905 25600.00 16200.00 0.00 0.03953 25800.00 16200.00 0.00 0.03992 25600.00 16400.00 0.00 0.04044 25800.00 16400.00 0.00 0.04078 25600.00 16600.00 0.00 0.04142 25800.00 16600.00 0.00 0.04170 25600.00 16800.00 0.00 0.04233 25800.00 16800.00 0.00 0.04255 26200.00 16800.00 0.00 0.04257 25600.00 17000.00 0.00 0.04305 25800.00 17000.00 0.00 0.04329 26000.00 17000.00 0.00 0.04331 26200.00 17000.00 0.00 0.04338 25600.00 17200.00 0.00 0.04385 25800.00 17200.00 0.00 0.04417 26000.00 17200.00 0.00 0.04424 26200.00 17200.00 0.00 0.04429 25600.00 17400.00 0.00 0.04481 25800.00 17400.00 0.00 0.04515 26000.00 17400.00 0.00 0.04519 25600.00 17600.00 0.00 0.04582 25800.00 17600.00 0.00 0.04614 26000.00 17600.00 0.00 0.04615 25600.00 17800.00 0.00 0.04696 25800.00 17800.00 0.00 0.04729 26000.00 17800.00 0.00 0.04731 25600.00 18000.00 0.00 0.04812 25800.00 18000.00 0.00 0.04846 26000.00 18000.00 0.00 0.04848 25600.00 18200.00 0.00 0.04924 25800.00 18200.00 0.00 0.04957 25600.00 18400.00 0.00 0.05027 25800.00 18400.00 0.00 0.05058 25600.00 18600.00 0.00 0.05134 25800.00 18600.00 0.00 0.05163 25600.00 18800.00 0.00 0.05243 25800.00 18800.00 0.00 0.05269 25600.00 19000.00 0.00 0.05338 25800.00 19000.00 0.00 0.05359 25600.00 19200.00 0.00 0.05445 25800.00 19200.00 0.00 0.05465 25600.00 19400.00 0.00 0.05555 25800.00 19400.00 0.00 0.05579 25600.00 19600.00 0.00 0.05670 25800.00 19600.00 0.00 0.05697 25600.00 19800.00 0.00 0.05796 25800.00 19800.00 0.00 0.05819 25400.00 20000.00 0.00 0.05820 25600.00 20000.00 0.00 0.05916 25800.00 20000.00 0.00 0.05934 25600.00 20200.00 0.00 0.06027 25800.00 20200.00 0.00 0.06040 25400.00 20400.00 0.00 0.06048 25600.00 20400.00 0.00 0.06142 25800.00 20400.00 0.00 0.06153 25400.00 20600.00 0.00 0.06182 25600.00 20600.00 0.00 0.06277 25800.00 20600.00 0.00 0.06286 25400.00 20800.00 0.00 0.06319 25600.00 20800.00 0.00 0.06411 25800.00 20800.00 0.00 0.06418 25400.00 21000.00 0.00 0.06457 25600.00 21000.00 0.00 0.06546 25800.00 21000.00 0.00 0.06551 25400.00 21200.00 0.00 0.06598 25600.00 21200.00 0.00 0.06684 25800.00 21200.00 0.00 0.06685 25400.00 21400.00 0.00 0.06739 25600.00 21400.00 0.00 0.06824 25400.00 21600.00 0.00 0.06872 25600.00 21600.00 0.00 0.06957 25400.00 21800.00 0.00 0.07010 25600.00 21800.00 0.00 0.07093 25400.00 22000.00 0.00 0.07157 25600.00 22000.00 0.00 0.07234 25400.00 22200.00 0.00 0.07295 25600.00 22200.00 0.00 0.07369 25400.00 22400.00 0.00 0.07439 25600.00 22400.00 0.00 0.07513 25400.00 22600.00 0.00 0.07577 25600.00 22600.00 0.00 0.07653 25400.00 22800.00 0.00 0.07731 25600.00 22800.00 0.00 0.07805 25400.00 23000.00 0.00 0.07885 25600.00 23000.00 0.00 0.07955 25400.00 23200.00 0.00 0.08056 25600.00 23200.00 0.00 0.08122 25400.00 23400.00 0.00 0.08248 25600.00 23400.00 0.00 0.08307 25400.00 23600.00 0.00 0.08426 25600.00 23600.00 0.00 0.08478 25400.00 23800.00 0.00 0.08617 25600.00 23800.00 0.00 0.08663 25400.00 24000.00 0.00 0.08801 25600.00 24000.00 0.00 0.08836 25400.00 24200.00 0.00 0.08975 25600.00 24200.00 0.00 0.08991 25400.00 24400.00 0.00 0.09118 25600.00 24400.00 0.00 0.09122 25400.00 24600.00 0.00 0.09264 25200.00 24800.00 0.00 0.09270 25400.00 24800.00 0.00 0.09411 25400.00 25000.00 0.00 0.09515 25200.00 25200.00 0.00 0.09524 25400.00 25200.00 0.00 0.09615 25200.00 25400.00 0.00 0.09620 25400.00 25400.00 0.00 0.09671 25200.00 25600.00 5.00 0.09673 25400.00 25600.00 5.00 0.09674 25200.00 25600.00 10.00 0.09693 25200.00 25600.00 15.00 0.09709 25200.00 25600.00 20.00 0.09720 25200.00 25600.00 25.00 0.09725 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25251.52 25561.69 25.00 0.09732 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/410.ctf NX, NY, NZ: 512 512 1 MODE : real