CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/409.mrc Output diagnostic file name Micrographs_0601/409.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/409.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2694.000 8426.000 Mean, RMS : 4473.716 223.4863 TITLE 1: emi2mrc: 409.emi Mon Jan 06 13:59:26 2014 TITLE 2: kV 300, Mag 78000x, Def 25430, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 292.664, Y 862.152, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.02401 13600.00 10000.00 0.00 -0.02387 13800.00 10000.00 0.00 -0.02353 14000.00 10000.00 0.00 -0.02318 14200.00 10000.00 0.00 -0.02297 14400.00 10000.00 0.00 -0.02254 14600.00 10000.00 0.00 -0.02195 14800.00 10000.00 0.00 -0.02145 15000.00 10000.00 0.00 -0.02100 15200.00 10000.00 0.00 -0.02053 15400.00 10000.00 0.00 -0.01997 15600.00 10000.00 0.00 -0.01943 15800.00 10000.00 0.00 -0.01883 16000.00 10000.00 0.00 -0.01839 16200.00 10000.00 0.00 -0.01797 16400.00 10000.00 0.00 -0.01740 16600.00 10000.00 0.00 -0.01696 16800.00 10000.00 0.00 -0.01642 17000.00 10000.00 0.00 -0.01578 17200.00 10000.00 0.00 -0.01508 17400.00 10000.00 0.00 -0.01438 17600.00 10000.00 0.00 -0.01365 17800.00 10000.00 0.00 -0.01271 18000.00 10000.00 0.00 -0.01185 18200.00 10000.00 0.00 -0.01101 18400.00 10000.00 0.00 -0.01015 18600.00 10000.00 0.00 -0.00938 18800.00 10000.00 0.00 -0.00863 19000.00 10000.00 0.00 -0.00776 19200.00 10000.00 0.00 -0.00678 19400.00 10000.00 0.00 -0.00589 19600.00 10000.00 0.00 -0.00487 19800.00 10000.00 0.00 -0.00374 20000.00 10000.00 0.00 -0.00274 20200.00 10000.00 0.00 -0.00185 20400.00 10000.00 0.00 -0.00105 20600.00 10000.00 0.00 -0.00022 20800.00 10000.00 0.00 0.00075 21000.00 10000.00 0.00 0.00178 21200.00 10000.00 0.00 0.00272 21400.00 10000.00 0.00 0.00363 21600.00 10000.00 0.00 0.00467 21800.00 10000.00 0.00 0.00583 22000.00 10000.00 0.00 0.00694 22200.00 10000.00 0.00 0.00785 22400.00 10000.00 0.00 0.00877 22600.00 10000.00 0.00 0.00980 22800.00 10000.00 0.00 0.01080 23000.00 10000.00 0.00 0.01189 23200.00 10000.00 0.00 0.01311 23400.00 10000.00 0.00 0.01444 23600.00 10000.00 0.00 0.01575 23800.00 10000.00 0.00 0.01701 24000.00 10000.00 0.00 0.01841 24200.00 10000.00 0.00 0.02001 24400.00 10000.00 0.00 0.02162 24600.00 10000.00 0.00 0.02291 24800.00 10000.00 0.00 0.02377 25000.00 10000.00 0.00 0.02425 25200.00 10000.00 0.00 0.02456 25400.00 10000.00 0.00 0.02489 25600.00 10000.00 0.00 0.02511 25800.00 10000.00 0.00 0.02521 26000.00 10000.00 0.00 0.02527 25800.00 10200.00 0.00 0.02536 26000.00 10200.00 0.00 0.02542 25600.00 10400.00 0.00 0.02547 25800.00 10400.00 0.00 0.02558 26000.00 10400.00 0.00 0.02564 25400.00 10600.00 0.00 0.02573 25600.00 10600.00 0.00 0.02597 25800.00 10600.00 0.00 0.02607 26000.00 10600.00 0.00 0.02611 25400.00 10800.00 0.00 0.02624 25600.00 10800.00 0.00 0.02645 25800.00 10800.00 0.00 0.02653 26000.00 10800.00 0.00 0.02654 25400.00 11000.00 0.00 0.02664 25600.00 11000.00 0.00 0.02684 25800.00 11000.00 0.00 0.02693 25400.00 11200.00 0.00 0.02707 25600.00 11200.00 0.00 0.02729 25800.00 11200.00 0.00 0.02739 25400.00 11400.00 0.00 0.02754 25600.00 11400.00 0.00 0.02778 25800.00 11400.00 0.00 0.02784 25400.00 11600.00 0.00 0.02805 25600.00 11600.00 0.00 0.02826 25400.00 11800.00 0.00 0.02857 25600.00 11800.00 0.00 0.02871 25400.00 12000.00 0.00 0.02906 25600.00 12000.00 0.00 0.02914 25200.00 12200.00 0.00 0.02925 25400.00 12200.00 0.00 0.02956 25600.00 12200.00 0.00 0.02960 25200.00 12400.00 0.00 0.02979 25400.00 12400.00 0.00 0.03007 25600.00 12400.00 0.00 0.03010 25200.00 12600.00 0.00 0.03033 25400.00 12600.00 0.00 0.03061 25600.00 12600.00 0.00 0.03064 25200.00 12800.00 0.00 0.03091 25400.00 12800.00 0.00 0.03123 25600.00 12800.00 0.00 0.03124 25200.00 13000.00 0.00 0.03156 25400.00 13000.00 0.00 0.03187 25200.00 13200.00 0.00 0.03215 25400.00 13200.00 0.00 0.03242 25200.00 13400.00 0.00 0.03277 25400.00 13400.00 0.00 0.03300 25000.00 13600.00 0.00 0.03302 25200.00 13600.00 0.00 0.03347 25400.00 13600.00 0.00 0.03366 25200.00 13800.00 0.00 0.03406 25400.00 13800.00 0.00 0.03418 25000.00 14000.00 0.00 0.03430 25200.00 14000.00 0.00 0.03468 25400.00 14000.00 0.00 0.03479 25000.00 14200.00 0.00 0.03507 25200.00 14200.00 0.00 0.03544 25400.00 14200.00 0.00 0.03559 25000.00 14400.00 0.00 0.03585 25200.00 14400.00 0.00 0.03621 25400.00 14400.00 0.00 0.03636 25000.00 14600.00 0.00 0.03661 25200.00 14600.00 0.00 0.03693 25400.00 14600.00 0.00 0.03708 25000.00 14800.00 0.00 0.03733 25200.00 14800.00 0.00 0.03763 25400.00 14800.00 0.00 0.03779 25000.00 15000.00 0.00 0.03819 25200.00 15000.00 0.00 0.03848 25400.00 15000.00 0.00 0.03864 25000.00 15200.00 0.00 0.03901 25200.00 15200.00 0.00 0.03929 25400.00 15200.00 0.00 0.03944 25000.00 15400.00 0.00 0.03983 25200.00 15400.00 0.00 0.04012 25400.00 15400.00 0.00 0.04029 24800.00 15600.00 0.00 0.04032 25000.00 15600.00 0.00 0.04073 25200.00 15600.00 0.00 0.04103 25400.00 15600.00 0.00 0.04123 25000.00 15800.00 0.00 0.04155 25200.00 15800.00 0.00 0.04189 25400.00 15800.00 0.00 0.04209 25000.00 16000.00 0.00 0.04241 25200.00 16000.00 0.00 0.04279 25400.00 16000.00 0.00 0.04297 25000.00 16200.00 0.00 0.04330 25200.00 16200.00 0.00 0.04371 25400.00 16200.00 0.00 0.04387 24800.00 16400.00 0.00 0.04389 25000.00 16400.00 0.00 0.04442 25200.00 16400.00 0.00 0.04482 25400.00 16400.00 0.00 0.04494 24800.00 16600.00 0.00 0.04503 25000.00 16600.00 0.00 0.04555 25200.00 16600.00 0.00 0.04592 25400.00 16600.00 0.00 0.04603 24800.00 16800.00 0.00 0.04624 25000.00 16800.00 0.00 0.04674 25200.00 16800.00 0.00 0.04710 25400.00 16800.00 0.00 0.04720 24800.00 17000.00 0.00 0.04757 25000.00 17000.00 0.00 0.04806 25200.00 17000.00 0.00 0.04843 25400.00 17000.00 0.00 0.04849 24800.00 17200.00 0.00 0.04883 25000.00 17200.00 0.00 0.04932 25200.00 17200.00 0.00 0.04966 24800.00 17400.00 0.00 0.05008 25000.00 17400.00 0.00 0.05055 25200.00 17400.00 0.00 0.05084 24800.00 17600.00 0.00 0.05121 25000.00 17600.00 0.00 0.05166 25200.00 17600.00 0.00 0.05191 24800.00 17800.00 0.00 0.05234 25000.00 17800.00 0.00 0.05278 25200.00 17800.00 0.00 0.05304 24800.00 18000.00 0.00 0.05345 25000.00 18000.00 0.00 0.05390 25200.00 18000.00 0.00 0.05415 24800.00 18200.00 0.00 0.05458 25000.00 18200.00 0.00 0.05503 25200.00 18200.00 0.00 0.05525 24800.00 18400.00 0.00 0.05587 25000.00 18400.00 0.00 0.05630 25200.00 18400.00 0.00 0.05647 24800.00 18600.00 0.00 0.05720 25000.00 18600.00 0.00 0.05758 25200.00 18600.00 0.00 0.05771 24600.00 18800.00 0.00 0.05777 24800.00 18800.00 0.00 0.05867 25000.00 18800.00 0.00 0.05900 25200.00 18800.00 0.00 0.05909 24600.00 19000.00 0.00 0.05918 24800.00 19000.00 0.00 0.06004 25000.00 19000.00 0.00 0.06031 25200.00 19000.00 0.00 0.06038 24600.00 19200.00 0.00 0.06054 24800.00 19200.00 0.00 0.06136 25000.00 19200.00 0.00 0.06159 25200.00 19200.00 0.00 0.06165 24600.00 19400.00 0.00 0.06193 24800.00 19400.00 0.00 0.06270 25000.00 19400.00 0.00 0.06291 25200.00 19400.00 0.00 0.06300 24600.00 19600.00 0.00 0.06323 24800.00 19600.00 0.00 0.06398 25000.00 19600.00 0.00 0.06422 25200.00 19600.00 0.00 0.06434 24600.00 19800.00 0.00 0.06452 24800.00 19800.00 0.00 0.06531 25000.00 19800.00 0.00 0.06560 25200.00 19800.00 0.00 0.06573 24600.00 20000.00 0.00 0.06593 24800.00 20000.00 0.00 0.06676 25000.00 20000.00 0.00 0.06705 25200.00 20000.00 0.00 0.06712 24600.00 20200.00 0.00 0.06734 24800.00 20200.00 0.00 0.06816 25000.00 20200.00 0.00 0.06841 24600.00 20400.00 0.00 0.06867 24800.00 20400.00 0.00 0.06948 25000.00 20400.00 0.00 0.06968 24600.00 20600.00 0.00 0.07012 24800.00 20600.00 0.00 0.07088 25000.00 20600.00 0.00 0.07103 24600.00 20800.00 0.00 0.07159 24800.00 20800.00 0.00 0.07232 25000.00 20800.00 0.00 0.07244 24600.00 21000.00 0.00 0.07305 24800.00 21000.00 0.00 0.07379 25000.00 21000.00 0.00 0.07388 24600.00 21200.00 0.00 0.07456 24800.00 21200.00 0.00 0.07527 25000.00 21200.00 0.00 0.07527 24600.00 21400.00 0.00 0.07612 24800.00 21400.00 0.00 0.07673 24600.00 21600.00 0.00 0.07754 24800.00 21600.00 0.00 0.07811 24600.00 21800.00 0.00 0.07911 24800.00 21800.00 0.00 0.07966 24600.00 22000.00 0.00 0.08081 24800.00 22000.00 0.00 0.08133 24600.00 22200.00 0.00 0.08247 24800.00 22200.00 0.00 0.08295 24400.00 22400.00 0.00 0.08297 24600.00 22400.00 0.00 0.08434 24800.00 22400.00 0.00 0.08476 24600.00 22600.00 0.00 0.08614 24800.00 22600.00 0.00 0.08650 24400.00 22800.00 0.00 0.08675 24600.00 22800.00 0.00 0.08810 24800.00 22800.00 0.00 0.08836 24400.00 23000.00 0.00 0.08891 24600.00 23000.00 0.00 0.09014 24800.00 23000.00 0.00 0.09028 24400.00 23200.00 0.00 0.09105 24600.00 23200.00 0.00 0.09221 24800.00 23200.00 0.00 0.09226 24400.00 23400.00 0.00 0.09319 24600.00 23400.00 0.00 0.09431 24400.00 23600.00 0.00 0.09518 24600.00 23600.00 0.00 0.09629 24400.00 23800.00 0.00 0.09752 24600.00 23800.00 0.00 0.09854 24400.00 24000.00 0.00 0.09979 24600.00 24000.00 0.00 0.10065 24400.00 24200.00 0.00 0.10202 24600.00 24200.00 0.00 0.10254 24400.00 24400.00 0.00 0.10396 24600.00 24400.00 0.00 0.10400 24400.00 24600.00 0.00 0.10506 24400.00 24600.00 5.00 0.10515 24400.00 24600.00 10.00 0.10523 24400.00 24600.00 15.00 0.10527 24400.00 24600.00 20.00 0.10529 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24400.00 24600.00 20.00 0.10529 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/409.ctf NX, NY, NZ: 512 512 1 MODE : real