CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/408.mrc Output diagnostic file name Micrographs_0601/408.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/408.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2224.000 8154.000 Mean, RMS : 4479.033 223.8291 TITLE 1: emi2mrc: 408.emi Mon Jan 06 13:57:49 2014 TITLE 2: kV 300, Mag 78000x, Def 25430, Spot 4, Int 55.05%, Obj 88.10%, Dif 73.67% TITLE 3: X 296.295, Y 858.011, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01331 10400.00 10000.00 0.00 -0.01322 10600.00 10000.00 0.00 -0.01318 10800.00 10000.00 0.00 -0.01317 11000.00 10000.00 0.00 -0.01309 11200.00 10000.00 0.00 -0.01291 11400.00 10000.00 0.00 -0.01281 11600.00 10000.00 0.00 -0.01270 11800.00 10000.00 0.00 -0.01245 12000.00 10000.00 0.00 -0.01212 12200.00 10000.00 0.00 -0.01167 12400.00 10000.00 0.00 -0.01117 12600.00 10000.00 0.00 -0.01067 12800.00 10000.00 0.00 -0.01014 13000.00 10000.00 0.00 -0.00955 13200.00 10000.00 0.00 -0.00898 13400.00 10000.00 0.00 -0.00830 13600.00 10000.00 0.00 -0.00759 13800.00 10000.00 0.00 -0.00690 14000.00 10000.00 0.00 -0.00614 14200.00 10000.00 0.00 -0.00537 14400.00 10000.00 0.00 -0.00450 14600.00 10000.00 0.00 -0.00365 14800.00 10000.00 0.00 -0.00279 15000.00 10000.00 0.00 -0.00194 15200.00 10000.00 0.00 -0.00114 15400.00 10000.00 0.00 -0.00015 15600.00 10000.00 0.00 0.00094 15800.00 10000.00 0.00 0.00204 16000.00 10000.00 0.00 0.00316 16200.00 10000.00 0.00 0.00438 16400.00 10000.00 0.00 0.00576 16600.00 10000.00 0.00 0.00705 16800.00 10000.00 0.00 0.00830 17000.00 10000.00 0.00 0.00957 17200.00 10000.00 0.00 0.01084 17400.00 10000.00 0.00 0.01208 17600.00 10000.00 0.00 0.01329 17800.00 10000.00 0.00 0.01464 18000.00 10000.00 0.00 0.01611 18200.00 10000.00 0.00 0.01769 18400.00 10000.00 0.00 0.01917 18600.00 10000.00 0.00 0.02057 18800.00 10000.00 0.00 0.02214 19000.00 10000.00 0.00 0.02377 19200.00 10000.00 0.00 0.02551 19400.00 10000.00 0.00 0.02728 19600.00 10000.00 0.00 0.02912 19800.00 10000.00 0.00 0.03119 20000.00 10000.00 0.00 0.03324 20200.00 10000.00 0.00 0.03536 20400.00 10000.00 0.00 0.03749 20600.00 10000.00 0.00 0.03975 20800.00 10000.00 0.00 0.04217 21000.00 10000.00 0.00 0.04408 21200.00 10000.00 0.00 0.04502 21400.00 10000.00 0.00 0.04534 21600.00 10000.00 0.00 0.04554 21800.00 10000.00 0.00 0.04556 21200.00 10200.00 0.00 0.04565 21400.00 10200.00 0.00 0.04596 21600.00 10200.00 0.00 0.04619 21800.00 10200.00 0.00 0.04620 21200.00 10400.00 0.00 0.04630 21400.00 10400.00 0.00 0.04665 21600.00 10400.00 0.00 0.04689 21200.00 10600.00 0.00 0.04697 21400.00 10600.00 0.00 0.04733 21600.00 10600.00 0.00 0.04753 21200.00 10800.00 0.00 0.04756 21400.00 10800.00 0.00 0.04792 21600.00 10800.00 0.00 0.04809 21200.00 11000.00 0.00 0.04828 21400.00 11000.00 0.00 0.04866 21600.00 11000.00 0.00 0.04883 21200.00 11200.00 0.00 0.04899 21400.00 11200.00 0.00 0.04937 21600.00 11200.00 0.00 0.04953 21800.00 11200.00 0.00 0.04954 21200.00 11400.00 0.00 0.04966 21400.00 11400.00 0.00 0.05002 21600.00 11400.00 0.00 0.05018 21800.00 11400.00 0.00 0.05019 21200.00 11600.00 0.00 0.05041 21400.00 11600.00 0.00 0.05076 21600.00 11600.00 0.00 0.05093 21800.00 11600.00 0.00 0.05094 21200.00 11800.00 0.00 0.05136 21400.00 11800.00 0.00 0.05171 21600.00 11800.00 0.00 0.05188 21200.00 12000.00 0.00 0.05241 21400.00 12000.00 0.00 0.05274 21600.00 12000.00 0.00 0.05289 21200.00 12200.00 0.00 0.05345 21400.00 12200.00 0.00 0.05377 21600.00 12200.00 0.00 0.05391 21200.00 12400.00 0.00 0.05454 21400.00 12400.00 0.00 0.05484 21600.00 12400.00 0.00 0.05498 21200.00 12600.00 0.00 0.05561 21400.00 12600.00 0.00 0.05589 21600.00 12600.00 0.00 0.05602 21200.00 12800.00 0.00 0.05674 21400.00 12800.00 0.00 0.05701 21600.00 12800.00 0.00 0.05711 21200.00 13000.00 0.00 0.05783 21400.00 13000.00 0.00 0.05808 21600.00 13000.00 0.00 0.05815 21200.00 13200.00 0.00 0.05896 21400.00 13200.00 0.00 0.05918 21600.00 13200.00 0.00 0.05923 21200.00 13400.00 0.00 0.06010 21400.00 13400.00 0.00 0.06029 21600.00 13400.00 0.00 0.06033 21200.00 13600.00 0.00 0.06116 21400.00 13600.00 0.00 0.06133 21600.00 13600.00 0.00 0.06135 21000.00 13800.00 0.00 0.06138 21200.00 13800.00 0.00 0.06218 21400.00 13800.00 0.00 0.06234 21600.00 13800.00 0.00 0.06235 21000.00 14000.00 0.00 0.06239 21200.00 14000.00 0.00 0.06319 21400.00 14000.00 0.00 0.06336 21600.00 14000.00 0.00 0.06336 21000.00 14200.00 0.00 0.06343 21200.00 14200.00 0.00 0.06424 21400.00 14200.00 0.00 0.06441 21000.00 14400.00 0.00 0.06455 21200.00 14400.00 0.00 0.06537 21400.00 14400.00 0.00 0.06553 21000.00 14600.00 0.00 0.06587 21200.00 14600.00 0.00 0.06672 21400.00 14600.00 0.00 0.06691 21000.00 14800.00 0.00 0.06728 21200.00 14800.00 0.00 0.06815 21400.00 14800.00 0.00 0.06834 21000.00 15000.00 0.00 0.06873 21200.00 15000.00 0.00 0.06959 21400.00 15000.00 0.00 0.06971 21000.00 15200.00 0.00 0.07016 21200.00 15200.00 0.00 0.07098 21400.00 15200.00 0.00 0.07102 21000.00 15400.00 0.00 0.07171 21200.00 15400.00 0.00 0.07247 21400.00 15400.00 0.00 0.07247 21000.00 15600.00 0.00 0.07333 21200.00 15600.00 0.00 0.07404 21000.00 15800.00 0.00 0.07488 21200.00 15800.00 0.00 0.07558 21000.00 16000.00 0.00 0.07660 21200.00 16000.00 0.00 0.07731 21000.00 16200.00 0.00 0.07835 21200.00 16200.00 0.00 0.07905 21000.00 16400.00 0.00 0.08015 21200.00 16400.00 0.00 0.08081 21000.00 16600.00 0.00 0.08185 21200.00 16600.00 0.00 0.08247 21000.00 16800.00 0.00 0.08356 21200.00 16800.00 0.00 0.08416 21000.00 17000.00 0.00 0.08535 21200.00 17000.00 0.00 0.08595 21000.00 17200.00 0.00 0.08715 21200.00 17200.00 0.00 0.08773 21000.00 17400.00 0.00 0.08900 21200.00 17400.00 0.00 0.08953 21000.00 17600.00 0.00 0.09073 21200.00 17600.00 0.00 0.09119 21000.00 17800.00 0.00 0.09256 21200.00 17800.00 0.00 0.09296 21000.00 18000.00 0.00 0.09436 21200.00 18000.00 0.00 0.09472 21000.00 18200.00 0.00 0.09621 21200.00 18200.00 0.00 0.09651 21000.00 18400.00 0.00 0.09802 21200.00 18400.00 0.00 0.09824 21000.00 18600.00 0.00 0.09978 21200.00 18600.00 0.00 0.09992 20800.00 18800.00 0.00 0.09998 21000.00 18800.00 0.00 0.10163 21200.00 18800.00 0.00 0.10171 20800.00 19000.00 0.00 0.10178 21000.00 19000.00 0.00 0.10343 21200.00 19000.00 0.00 0.10344 20800.00 19200.00 0.00 0.10390 21000.00 19200.00 0.00 0.10551 20800.00 19400.00 0.00 0.10592 21000.00 19400.00 0.00 0.10744 20800.00 19600.00 0.00 0.10796 21000.00 19600.00 0.00 0.10935 20800.00 19800.00 0.00 0.10995 21000.00 19800.00 0.00 0.11122 20800.00 20000.00 0.00 0.11191 21000.00 20000.00 0.00 0.11305 20800.00 20200.00 0.00 0.11392 21000.00 20200.00 0.00 0.11490 20800.00 20400.00 0.00 0.11575 21000.00 20400.00 0.00 0.11651 20800.00 20600.00 0.00 0.11770 21000.00 20600.00 0.00 0.11809 20800.00 20800.00 0.00 0.11906 21000.00 20800.00 0.00 0.11915 20800.00 21000.00 0.00 0.12020 20800.00 21000.00 5.00 0.12028 20800.00 21000.00 10.00 0.12035 20800.00 21000.00 15.00 0.12040 20800.00 21000.00 20.00 0.12042 20800.00 21000.00 25.00 0.12042 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20831.68 21089.25 15.13 0.12063 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/408.ctf NX, NY, NZ: 512 512 1 MODE : real