CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/406.mrc Output diagnostic file name Micrographs_0601/406.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/406.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2422.000 8376.000 Mean, RMS : 4449.854 219.3725 TITLE 1: emi2mrc: 406.emi Mon Jan 06 11:18:31 2014 TITLE 2: kV 300, Mag 78000x, Def 25100, Spot 4, Int 55.07%, Obj 88.05%, Dif 73.67% TITLE 3: X 65.657, Y 569.662, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00430 10200.00 10000.00 0.00 -0.00419 10400.00 10000.00 0.00 -0.00404 10600.00 10000.00 0.00 -0.00373 10800.00 10000.00 0.00 -0.00338 11000.00 10000.00 0.00 -0.00321 11200.00 10000.00 0.00 -0.00313 11400.00 10000.00 0.00 -0.00295 11600.00 10000.00 0.00 -0.00288 12200.00 10000.00 0.00 -0.00270 12400.00 10000.00 0.00 -0.00262 12600.00 10000.00 0.00 -0.00251 12800.00 10000.00 0.00 -0.00234 13000.00 10000.00 0.00 -0.00222 13200.00 10000.00 0.00 -0.00203 13400.00 10000.00 0.00 -0.00173 13600.00 10000.00 0.00 -0.00135 13800.00 10000.00 0.00 -0.00101 14000.00 10000.00 0.00 -0.00067 14200.00 10000.00 0.00 -0.00025 14400.00 10000.00 0.00 0.00011 14600.00 10000.00 0.00 0.00037 14800.00 10000.00 0.00 0.00088 15000.00 10000.00 0.00 0.00161 15200.00 10000.00 0.00 0.00224 15400.00 10000.00 0.00 0.00291 15600.00 10000.00 0.00 0.00369 15800.00 10000.00 0.00 0.00459 16000.00 10000.00 0.00 0.00568 16200.00 10000.00 0.00 0.00677 16400.00 10000.00 0.00 0.00784 16600.00 10000.00 0.00 0.00900 16800.00 10000.00 0.00 0.01006 17000.00 10000.00 0.00 0.01112 17200.00 10000.00 0.00 0.01228 17400.00 10000.00 0.00 0.01343 17600.00 10000.00 0.00 0.01465 17800.00 10000.00 0.00 0.01585 18000.00 10000.00 0.00 0.01695 18200.00 10000.00 0.00 0.01805 18400.00 10000.00 0.00 0.01916 18600.00 10000.00 0.00 0.02040 18800.00 10000.00 0.00 0.02174 19000.00 10000.00 0.00 0.02300 19200.00 10000.00 0.00 0.02414 19400.00 10000.00 0.00 0.02516 19600.00 10000.00 0.00 0.02627 19800.00 10000.00 0.00 0.02751 20000.00 10000.00 0.00 0.02882 20200.00 10000.00 0.00 0.03032 20400.00 10000.00 0.00 0.03184 20600.00 10000.00 0.00 0.03332 20800.00 10000.00 0.00 0.03502 21000.00 10000.00 0.00 0.03698 21200.00 10000.00 0.00 0.03893 21400.00 10000.00 0.00 0.04089 21600.00 10000.00 0.00 0.04291 21800.00 10000.00 0.00 0.04496 22000.00 10000.00 0.00 0.04716 22200.00 10000.00 0.00 0.04885 22400.00 10000.00 0.00 0.04969 22600.00 10000.00 0.00 0.05010 22800.00 10000.00 0.00 0.05031 22600.00 10200.00 0.00 0.05049 22800.00 10200.00 0.00 0.05074 23000.00 10200.00 0.00 0.05074 22600.00 10400.00 0.00 0.05117 22800.00 10400.00 0.00 0.05148 23000.00 10400.00 0.00 0.05150 22600.00 10600.00 0.00 0.05183 22800.00 10600.00 0.00 0.05214 22600.00 10800.00 0.00 0.05245 22800.00 10800.00 0.00 0.05274 22600.00 11000.00 0.00 0.05323 22800.00 11000.00 0.00 0.05348 22600.00 11200.00 0.00 0.05387 22800.00 11200.00 0.00 0.05406 22600.00 11400.00 0.00 0.05448 22800.00 11400.00 0.00 0.05461 22600.00 11600.00 0.00 0.05504 22800.00 11600.00 0.00 0.05514 22600.00 11800.00 0.00 0.05559 22800.00 11800.00 0.00 0.05567 22400.00 12000.00 0.00 0.05578 22600.00 12000.00 0.00 0.05624 22800.00 12000.00 0.00 0.05629 22400.00 12200.00 0.00 0.05652 22600.00 12200.00 0.00 0.05696 22800.00 12200.00 0.00 0.05702 22400.00 12400.00 0.00 0.05732 22600.00 12400.00 0.00 0.05779 22800.00 12400.00 0.00 0.05790 22400.00 12600.00 0.00 0.05817 22600.00 12600.00 0.00 0.05867 22800.00 12600.00 0.00 0.05875 22400.00 12800.00 0.00 0.05899 22600.00 12800.00 0.00 0.05946 22800.00 12800.00 0.00 0.05947 22400.00 13000.00 0.00 0.05974 22600.00 13000.00 0.00 0.06018 22400.00 13200.00 0.00 0.06053 22600.00 13200.00 0.00 0.06096 22400.00 13400.00 0.00 0.06120 22600.00 13400.00 0.00 0.06165 22400.00 13600.00 0.00 0.06201 22600.00 13600.00 0.00 0.06246 22800.00 13600.00 0.00 0.06246 22400.00 13800.00 0.00 0.06296 22600.00 13800.00 0.00 0.06337 22400.00 14000.00 0.00 0.06385 22600.00 14000.00 0.00 0.06421 22400.00 14200.00 0.00 0.06483 22600.00 14200.00 0.00 0.06520 22800.00 14200.00 0.00 0.06524 22400.00 14400.00 0.00 0.06586 22600.00 14400.00 0.00 0.06629 22800.00 14400.00 0.00 0.06636 22400.00 14600.00 0.00 0.06709 22600.00 14600.00 0.00 0.06756 22800.00 14600.00 0.00 0.06763 22400.00 14800.00 0.00 0.06848 22600.00 14800.00 0.00 0.06898 22800.00 14800.00 0.00 0.06902 22400.00 15000.00 0.00 0.06990 22600.00 15000.00 0.00 0.07039 22400.00 15200.00 0.00 0.07122 22600.00 15200.00 0.00 0.07167 22400.00 15400.00 0.00 0.07259 22600.00 15400.00 0.00 0.07298 22200.00 15600.00 0.00 0.07304 22400.00 15600.00 0.00 0.07400 22600.00 15600.00 0.00 0.07429 22200.00 15800.00 0.00 0.07440 22400.00 15800.00 0.00 0.07530 22600.00 15800.00 0.00 0.07550 22200.00 16000.00 0.00 0.07588 22400.00 16000.00 0.00 0.07671 22600.00 16000.00 0.00 0.07685 22200.00 16200.00 0.00 0.07726 22400.00 16200.00 0.00 0.07801 22600.00 16200.00 0.00 0.07809 22200.00 16400.00 0.00 0.07843 22400.00 16400.00 0.00 0.07914 22600.00 16400.00 0.00 0.07920 22200.00 16600.00 0.00 0.07961 22400.00 16600.00 0.00 0.08034 22600.00 16600.00 0.00 0.08040 22200.00 16800.00 0.00 0.08100 22400.00 16800.00 0.00 0.08173 22600.00 16800.00 0.00 0.08177 22200.00 17000.00 0.00 0.08248 22400.00 17000.00 0.00 0.08320 22200.00 17200.00 0.00 0.08392 22400.00 17200.00 0.00 0.08460 22200.00 17400.00 0.00 0.08540 22400.00 17400.00 0.00 0.08610 22200.00 17600.00 0.00 0.08691 22400.00 17600.00 0.00 0.08765 22200.00 17800.00 0.00 0.08866 22400.00 17800.00 0.00 0.08940 22200.00 18000.00 0.00 0.09050 22400.00 18000.00 0.00 0.09122 22200.00 18200.00 0.00 0.09248 22400.00 18200.00 0.00 0.09316 22200.00 18400.00 0.00 0.09446 22400.00 18400.00 0.00 0.09507 22200.00 18600.00 0.00 0.09628 22400.00 18600.00 0.00 0.09684 22200.00 18800.00 0.00 0.09816 22400.00 18800.00 0.00 0.09867 22200.00 19000.00 0.00 0.10004 22400.00 19000.00 0.00 0.10054 22200.00 19200.00 0.00 0.10207 22400.00 19200.00 0.00 0.10251 22200.00 19400.00 0.00 0.10403 22400.00 19400.00 0.00 0.10438 22000.00 19600.00 0.00 0.10447 22200.00 19600.00 0.00 0.10606 22400.00 19600.00 0.00 0.10634 22000.00 19800.00 0.00 0.10656 22200.00 19800.00 0.00 0.10812 22400.00 19800.00 0.00 0.10831 22000.00 20000.00 0.00 0.10882 22200.00 20000.00 0.00 0.11031 22400.00 20000.00 0.00 0.11040 22000.00 20200.00 0.00 0.11119 22200.00 20200.00 0.00 0.11261 22400.00 20200.00 0.00 0.11264 22000.00 20400.00 0.00 0.11354 22200.00 20400.00 0.00 0.11491 22000.00 20600.00 0.00 0.11611 22200.00 20600.00 0.00 0.11743 22000.00 20800.00 0.00 0.11852 22200.00 20800.00 0.00 0.11973 22000.00 21000.00 0.00 0.12107 22200.00 21000.00 0.00 0.12210 22000.00 21200.00 0.00 0.12359 22200.00 21200.00 0.00 0.12442 22000.00 21400.00 0.00 0.12624 22200.00 21400.00 0.00 0.12684 22000.00 21600.00 0.00 0.12887 22200.00 21600.00 0.00 0.12916 22000.00 21800.00 0.00 0.13126 21800.00 22000.00 0.00 0.13151 22000.00 22000.00 0.00 0.13315 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21982.79 22092.43 34.14 0.13348 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/406.ctf NX, NY, NZ: 512 512 1 MODE : real