CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/402.mrc Output diagnostic file name Micrographs_0601/402.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/402.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2532.000 7728.000 Mean, RMS : 4559.962 223.3056 TITLE 1: emi2mrc: 402.emi Mon Jan 06 13:51:51 2014 TITLE 2: kV 300, Mag 78000x, Def 25760, Spot 4, Int 55.04%, Obj 88.07%, Dif 73.67% TITLE 3: X 305.649, Y 847.737, Z -25.472, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00652 10200.00 10000.00 0.00 -0.00633 10400.00 10000.00 0.00 -0.00600 10600.00 10000.00 0.00 -0.00561 10800.00 10000.00 0.00 -0.00533 11000.00 10000.00 0.00 -0.00521 11200.00 10000.00 0.00 -0.00497 11400.00 10000.00 0.00 -0.00454 11600.00 10000.00 0.00 -0.00408 11800.00 10000.00 0.00 -0.00352 12000.00 10000.00 0.00 -0.00299 12200.00 10000.00 0.00 -0.00248 12400.00 10000.00 0.00 -0.00186 12600.00 10000.00 0.00 -0.00123 12800.00 10000.00 0.00 -0.00050 13000.00 10000.00 0.00 0.00018 13200.00 10000.00 0.00 0.00074 13400.00 10000.00 0.00 0.00159 13600.00 10000.00 0.00 0.00254 13800.00 10000.00 0.00 0.00343 14000.00 10000.00 0.00 0.00436 14200.00 10000.00 0.00 0.00543 14400.00 10000.00 0.00 0.00665 14600.00 10000.00 0.00 0.00791 14800.00 10000.00 0.00 0.00914 15000.00 10000.00 0.00 0.01028 15200.00 10000.00 0.00 0.01141 15400.00 10000.00 0.00 0.01259 15600.00 10000.00 0.00 0.01389 15800.00 10000.00 0.00 0.01529 16000.00 10000.00 0.00 0.01673 16200.00 10000.00 0.00 0.01818 16400.00 10000.00 0.00 0.01962 16600.00 10000.00 0.00 0.02113 16800.00 10000.00 0.00 0.02269 17000.00 10000.00 0.00 0.02435 17200.00 10000.00 0.00 0.02602 17400.00 10000.00 0.00 0.02766 17600.00 10000.00 0.00 0.02932 17800.00 10000.00 0.00 0.03094 18000.00 10000.00 0.00 0.03269 18200.00 10000.00 0.00 0.03460 18400.00 10000.00 0.00 0.03652 18600.00 10000.00 0.00 0.03848 18800.00 10000.00 0.00 0.04050 19000.00 10000.00 0.00 0.04255 19200.00 10000.00 0.00 0.04464 19400.00 10000.00 0.00 0.04676 19600.00 10000.00 0.00 0.04855 19800.00 10000.00 0.00 0.04972 20000.00 10000.00 0.00 0.05043 20200.00 10000.00 0.00 0.05088 20400.00 10000.00 0.00 0.05125 20600.00 10000.00 0.00 0.05162 20800.00 10000.00 0.00 0.05176 20400.00 10200.00 0.00 0.05213 20600.00 10200.00 0.00 0.05252 20800.00 10200.00 0.00 0.05264 20200.00 10400.00 0.00 0.05268 20400.00 10400.00 0.00 0.05309 20600.00 10400.00 0.00 0.05346 20800.00 10400.00 0.00 0.05351 20200.00 10600.00 0.00 0.05367 20400.00 10600.00 0.00 0.05407 20600.00 10600.00 0.00 0.05442 20800.00 10600.00 0.00 0.05442 20200.00 10800.00 0.00 0.05467 20400.00 10800.00 0.00 0.05506 20600.00 10800.00 0.00 0.05539 20200.00 11000.00 0.00 0.05553 20400.00 11000.00 0.00 0.05593 20600.00 11000.00 0.00 0.05622 20200.00 11200.00 0.00 0.05636 20400.00 11200.00 0.00 0.05678 20600.00 11200.00 0.00 0.05702 20200.00 11400.00 0.00 0.05736 20400.00 11400.00 0.00 0.05776 20600.00 11400.00 0.00 0.05793 20200.00 11600.00 0.00 0.05823 20400.00 11600.00 0.00 0.05859 20600.00 11600.00 0.00 0.05870 20000.00 11800.00 0.00 0.05883 20200.00 11800.00 0.00 0.05925 20400.00 11800.00 0.00 0.05959 20600.00 11800.00 0.00 0.05968 20000.00 12000.00 0.00 0.05991 20200.00 12000.00 0.00 0.06033 20400.00 12000.00 0.00 0.06067 20600.00 12000.00 0.00 0.06072 20000.00 12200.00 0.00 0.06094 20200.00 12200.00 0.00 0.06137 20400.00 12200.00 0.00 0.06168 20000.00 12400.00 0.00 0.06202 20200.00 12400.00 0.00 0.06241 20400.00 12400.00 0.00 0.06267 20000.00 12600.00 0.00 0.06302 20200.00 12600.00 0.00 0.06338 20400.00 12600.00 0.00 0.06360 20000.00 12800.00 0.00 0.06420 20200.00 12800.00 0.00 0.06456 20400.00 12800.00 0.00 0.06477 19800.00 13000.00 0.00 0.06493 20000.00 13000.00 0.00 0.06552 20200.00 13000.00 0.00 0.06586 20400.00 13000.00 0.00 0.06601 19800.00 13200.00 0.00 0.06641 20000.00 13200.00 0.00 0.06695 20200.00 13200.00 0.00 0.06724 20400.00 13200.00 0.00 0.06730 19800.00 13400.00 0.00 0.06772 20000.00 13400.00 0.00 0.06821 20200.00 13400.00 0.00 0.06844 19800.00 13600.00 0.00 0.06889 20000.00 13600.00 0.00 0.06932 20200.00 13600.00 0.00 0.06949 19800.00 13800.00 0.00 0.07015 20000.00 13800.00 0.00 0.07053 20200.00 13800.00 0.00 0.07069 19800.00 14000.00 0.00 0.07153 20000.00 14000.00 0.00 0.07191 20200.00 14000.00 0.00 0.07209 19800.00 14200.00 0.00 0.07306 20000.00 14200.00 0.00 0.07346 20200.00 14200.00 0.00 0.07366 19800.00 14400.00 0.00 0.07457 20000.00 14400.00 0.00 0.07498 20200.00 14400.00 0.00 0.07515 19800.00 14600.00 0.00 0.07626 20000.00 14600.00 0.00 0.07662 20200.00 14600.00 0.00 0.07676 19600.00 14800.00 0.00 0.07686 19800.00 14800.00 0.00 0.07794 20000.00 14800.00 0.00 0.07824 20200.00 14800.00 0.00 0.07836 19600.00 15000.00 0.00 0.07867 19800.00 15000.00 0.00 0.07968 20000.00 15000.00 0.00 0.07993 20200.00 15000.00 0.00 0.08004 19600.00 15200.00 0.00 0.08045 19800.00 15200.00 0.00 0.08142 20000.00 15200.00 0.00 0.08167 20200.00 15200.00 0.00 0.08182 19600.00 15400.00 0.00 0.08224 19800.00 15400.00 0.00 0.08322 20000.00 15400.00 0.00 0.08351 20200.00 15400.00 0.00 0.08367 19600.00 15600.00 0.00 0.08417 19800.00 15600.00 0.00 0.08514 20000.00 15600.00 0.00 0.08543 20200.00 15600.00 0.00 0.08553 19600.00 15800.00 0.00 0.08606 19800.00 15800.00 0.00 0.08699 20000.00 15800.00 0.00 0.08723 20200.00 15800.00 0.00 0.08728 19600.00 16000.00 0.00 0.08818 19800.00 16000.00 0.00 0.08907 20000.00 16000.00 0.00 0.08930 20200.00 16000.00 0.00 0.08932 19600.00 16200.00 0.00 0.09029 19800.00 16200.00 0.00 0.09119 20000.00 16200.00 0.00 0.09142 19600.00 16400.00 0.00 0.09251 19800.00 16400.00 0.00 0.09344 20000.00 16400.00 0.00 0.09365 19600.00 16600.00 0.00 0.09470 19800.00 16600.00 0.00 0.09562 20000.00 16600.00 0.00 0.09578 19600.00 16800.00 0.00 0.09692 19800.00 16800.00 0.00 0.09781 20000.00 16800.00 0.00 0.09791 19600.00 17000.00 0.00 0.09935 19800.00 17000.00 0.00 0.10020 20000.00 17000.00 0.00 0.10024 19600.00 17200.00 0.00 0.10165 19800.00 17200.00 0.00 0.10245 19600.00 17400.00 0.00 0.10406 19800.00 17400.00 0.00 0.10486 19600.00 17600.00 0.00 0.10657 19800.00 17600.00 0.00 0.10733 19400.00 17800.00 0.00 0.10739 19600.00 17800.00 0.00 0.10926 19800.00 17800.00 0.00 0.10990 19400.00 18000.00 0.00 0.11007 19600.00 18000.00 0.00 0.11185 19800.00 18000.00 0.00 0.11236 19400.00 18200.00 0.00 0.11286 19600.00 18200.00 0.00 0.11459 19800.00 18200.00 0.00 0.11505 19400.00 18400.00 0.00 0.11581 19600.00 18400.00 0.00 0.11754 19800.00 18400.00 0.00 0.11796 19400.00 18600.00 0.00 0.11883 19600.00 18600.00 0.00 0.12053 19800.00 18600.00 0.00 0.12082 19400.00 18800.00 0.00 0.12216 19600.00 18800.00 0.00 0.12377 19800.00 18800.00 0.00 0.12383 19400.00 19000.00 0.00 0.12543 19600.00 19000.00 0.00 0.12688 19400.00 19200.00 0.00 0.12894 19600.00 19200.00 0.00 0.13006 19400.00 19400.00 0.00 0.13205 19600.00 19400.00 0.00 0.13271 19400.00 19600.00 0.00 0.13425 19600.00 19600.00 0.00 0.13432 19400.00 19600.00 5.00 0.13436 19400.00 19600.00 10.00 0.13444 19400.00 19600.00 15.00 0.13448 19400.00 19600.00 20.00 0.13449 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19443.09 19670.53 15.96 0.13481 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/402.ctf NX, NY, NZ: 512 512 1 MODE : real