CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/400.mrc Output diagnostic file name Micrographs_0601/400.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/400.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2612.000 8332.000 Mean, RMS : 4519.792 220.7834 TITLE 1: emi2mrc: 400.emi Sun Jan 05 23:45:37 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.07%, Obj 88.07%, Dif 73.67% TITLE 3: X 68.787, Y 572.337, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00406 10200.00 10000.00 0.00 -0.00404 10400.00 10000.00 0.00 -0.00396 10800.00 10000.00 0.00 -0.00395 11000.00 10000.00 0.00 -0.00391 11200.00 10000.00 0.00 -0.00382 11400.00 10000.00 0.00 -0.00364 11600.00 10000.00 0.00 -0.00327 11800.00 10000.00 0.00 -0.00294 12000.00 10000.00 0.00 -0.00265 12200.00 10000.00 0.00 -0.00214 12400.00 10000.00 0.00 -0.00177 12600.00 10000.00 0.00 -0.00155 12800.00 10000.00 0.00 -0.00125 13000.00 10000.00 0.00 -0.00078 13200.00 10000.00 0.00 -0.00026 13400.00 10000.00 0.00 0.00018 13600.00 10000.00 0.00 0.00068 13800.00 10000.00 0.00 0.00132 14000.00 10000.00 0.00 0.00209 14200.00 10000.00 0.00 0.00278 14400.00 10000.00 0.00 0.00331 14600.00 10000.00 0.00 0.00400 14800.00 10000.00 0.00 0.00478 15000.00 10000.00 0.00 0.00560 15200.00 10000.00 0.00 0.00646 15400.00 10000.00 0.00 0.00720 15600.00 10000.00 0.00 0.00803 15800.00 10000.00 0.00 0.00898 16000.00 10000.00 0.00 0.00996 16200.00 10000.00 0.00 0.01093 16400.00 10000.00 0.00 0.01197 16600.00 10000.00 0.00 0.01320 16800.00 10000.00 0.00 0.01451 17000.00 10000.00 0.00 0.01587 17200.00 10000.00 0.00 0.01716 17400.00 10000.00 0.00 0.01842 17600.00 10000.00 0.00 0.01977 17800.00 10000.00 0.00 0.02112 18000.00 10000.00 0.00 0.02252 18200.00 10000.00 0.00 0.02390 18400.00 10000.00 0.00 0.02523 18600.00 10000.00 0.00 0.02655 18800.00 10000.00 0.00 0.02802 19000.00 10000.00 0.00 0.02953 19200.00 10000.00 0.00 0.03098 19400.00 10000.00 0.00 0.03254 19600.00 10000.00 0.00 0.03408 19800.00 10000.00 0.00 0.03560 20000.00 10000.00 0.00 0.03739 20200.00 10000.00 0.00 0.03936 20400.00 10000.00 0.00 0.04132 20600.00 10000.00 0.00 0.04337 20800.00 10000.00 0.00 0.04552 21000.00 10000.00 0.00 0.04782 21200.00 10000.00 0.00 0.05022 21400.00 10000.00 0.00 0.05218 21600.00 10000.00 0.00 0.05328 21800.00 10000.00 0.00 0.05376 22000.00 10000.00 0.00 0.05412 22200.00 10000.00 0.00 0.05421 21800.00 10200.00 0.00 0.05438 22000.00 10200.00 0.00 0.05474 22200.00 10200.00 0.00 0.05476 21800.00 10400.00 0.00 0.05507 22000.00 10400.00 0.00 0.05537 21800.00 10600.00 0.00 0.05575 22000.00 10600.00 0.00 0.05600 21800.00 10800.00 0.00 0.05648 22000.00 10800.00 0.00 0.05670 21600.00 11000.00 0.00 0.05677 21800.00 11000.00 0.00 0.05725 22000.00 11000.00 0.00 0.05746 22200.00 11000.00 0.00 0.05747 21800.00 11200.00 0.00 0.05783 22000.00 11200.00 0.00 0.05806 22200.00 11200.00 0.00 0.05811 21800.00 11400.00 0.00 0.05853 22000.00 11400.00 0.00 0.05878 22200.00 11400.00 0.00 0.05884 21600.00 11600.00 0.00 0.05885 21800.00 11600.00 0.00 0.05940 22000.00 11600.00 0.00 0.05965 22200.00 11600.00 0.00 0.05966 21600.00 11800.00 0.00 0.05978 21800.00 11800.00 0.00 0.06034 22000.00 11800.00 0.00 0.06054 21600.00 12000.00 0.00 0.06076 21800.00 12000.00 0.00 0.06126 22000.00 12000.00 0.00 0.06141 21600.00 12200.00 0.00 0.06173 21800.00 12200.00 0.00 0.06216 22000.00 12200.00 0.00 0.06227 21600.00 12400.00 0.00 0.06264 21800.00 12400.00 0.00 0.06306 22000.00 12400.00 0.00 0.06320 21600.00 12600.00 0.00 0.06359 21800.00 12600.00 0.00 0.06402 22000.00 12600.00 0.00 0.06417 21600.00 12800.00 0.00 0.06460 21800.00 12800.00 0.00 0.06502 22000.00 12800.00 0.00 0.06513 21600.00 13000.00 0.00 0.06549 21800.00 13000.00 0.00 0.06587 22000.00 13000.00 0.00 0.06599 21600.00 13200.00 0.00 0.06645 21800.00 13200.00 0.00 0.06687 22000.00 13200.00 0.00 0.06701 21600.00 13400.00 0.00 0.06754 21800.00 13400.00 0.00 0.06800 22000.00 13400.00 0.00 0.06817 21600.00 13600.00 0.00 0.06876 21800.00 13600.00 0.00 0.06922 22000.00 13600.00 0.00 0.06936 21600.00 13800.00 0.00 0.06993 21800.00 13800.00 0.00 0.07037 22000.00 13800.00 0.00 0.07049 21600.00 14000.00 0.00 0.07106 21800.00 14000.00 0.00 0.07151 22000.00 14000.00 0.00 0.07162 21600.00 14200.00 0.00 0.07227 21800.00 14200.00 0.00 0.07274 22000.00 14200.00 0.00 0.07286 21600.00 14400.00 0.00 0.07351 21800.00 14400.00 0.00 0.07401 22000.00 14400.00 0.00 0.07410 21600.00 14600.00 0.00 0.07495 21800.00 14600.00 0.00 0.07541 22000.00 14600.00 0.00 0.07541 21600.00 14800.00 0.00 0.07623 21800.00 14800.00 0.00 0.07659 21600.00 15000.00 0.00 0.07746 21800.00 15000.00 0.00 0.07780 21600.00 15200.00 0.00 0.07879 21800.00 15200.00 0.00 0.07914 21600.00 15400.00 0.00 0.08024 21800.00 15400.00 0.00 0.08053 21400.00 15600.00 0.00 0.08067 21600.00 15600.00 0.00 0.08172 21800.00 15600.00 0.00 0.08195 21400.00 15800.00 0.00 0.08196 21600.00 15800.00 0.00 0.08299 21800.00 15800.00 0.00 0.08323 21400.00 16000.00 0.00 0.08344 21600.00 16000.00 0.00 0.08453 21800.00 16000.00 0.00 0.08477 21400.00 16200.00 0.00 0.08511 21600.00 16200.00 0.00 0.08617 21800.00 16200.00 0.00 0.08636 21400.00 16400.00 0.00 0.08679 21600.00 16400.00 0.00 0.08779 21800.00 16400.00 0.00 0.08791 21400.00 16600.00 0.00 0.08836 21600.00 16600.00 0.00 0.08934 21800.00 16600.00 0.00 0.08945 21400.00 16800.00 0.00 0.09002 21600.00 16800.00 0.00 0.09100 21800.00 16800.00 0.00 0.09109 21400.00 17000.00 0.00 0.09175 21600.00 17000.00 0.00 0.09270 21800.00 17000.00 0.00 0.09271 21400.00 17200.00 0.00 0.09339 21600.00 17200.00 0.00 0.09429 21400.00 17400.00 0.00 0.09522 21600.00 17400.00 0.00 0.09605 21400.00 17600.00 0.00 0.09697 21600.00 17600.00 0.00 0.09774 21400.00 17800.00 0.00 0.09880 21600.00 17800.00 0.00 0.09954 21400.00 18000.00 0.00 0.10061 21600.00 18000.00 0.00 0.10134 21400.00 18200.00 0.00 0.10238 21600.00 18200.00 0.00 0.10310 21400.00 18400.00 0.00 0.10420 21600.00 18400.00 0.00 0.10490 21400.00 18600.00 0.00 0.10606 21600.00 18600.00 0.00 0.10672 21400.00 18800.00 0.00 0.10801 21600.00 18800.00 0.00 0.10865 21400.00 19000.00 0.00 0.11002 21600.00 19000.00 0.00 0.11059 21400.00 19200.00 0.00 0.11213 21600.00 19200.00 0.00 0.11255 21400.00 19400.00 0.00 0.11398 21600.00 19400.00 0.00 0.11426 21200.00 19600.00 0.00 0.11431 21400.00 19600.00 0.00 0.11598 21600.00 19600.00 0.00 0.11616 21200.00 19800.00 0.00 0.11638 21400.00 19800.00 0.00 0.11798 21600.00 19800.00 0.00 0.11808 21200.00 20000.00 0.00 0.11848 21400.00 20000.00 0.00 0.12002 21200.00 20200.00 0.00 0.12075 21400.00 20200.00 0.00 0.12218 21200.00 20400.00 0.00 0.12294 21400.00 20400.00 0.00 0.12421 21200.00 20600.00 0.00 0.12533 21400.00 20600.00 0.00 0.12632 21200.00 20800.00 0.00 0.12734 21400.00 20800.00 0.00 0.12800 21200.00 21000.00 0.00 0.12944 21400.00 21000.00 0.00 0.12962 21200.00 21200.00 0.00 0.13078 21200.00 21400.00 5.00 0.13081 21200.00 21400.00 10.00 0.13086 21200.00 21400.00 15.00 0.13091 21200.00 21400.00 20.00 0.13095 21200.00 21400.00 25.00 0.13099 21200.00 21400.00 30.00 0.13101 21200.00 21400.00 35.00 0.13103 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21181.94 21391.54 36.96 0.13104 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/400.ctf NX, NY, NZ: 512 512 1 MODE : real