CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/396.mrc Output diagnostic file name Micrographs_0601/396.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/396.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2526.000 7860.000 Mean, RMS : 4522.110 219.1206 TITLE 1: emi2mrc: 396.emi Sun Jan 05 23:42:54 2014 TITLE 2: kV 300, Mag 78000x, Def 18540, Spot 4, Int 55.07%, Obj 88.07%, Dif 73.67% TITLE 3: X 58.307, Y 573.516, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00094 10200.00 10000.00 0.00 -0.00079 10400.00 10000.00 0.00 -0.00060 10600.00 10000.00 0.00 -0.00043 10800.00 10000.00 0.00 -0.00021 11000.00 10000.00 0.00 0.00003 11200.00 10000.00 0.00 0.00024 11400.00 10000.00 0.00 0.00038 11600.00 10000.00 0.00 0.00060 11800.00 10000.00 0.00 0.00088 12000.00 10000.00 0.00 0.00131 12200.00 10000.00 0.00 0.00190 12400.00 10000.00 0.00 0.00242 12600.00 10000.00 0.00 0.00294 12800.00 10000.00 0.00 0.00353 13000.00 10000.00 0.00 0.00407 13200.00 10000.00 0.00 0.00463 13400.00 10000.00 0.00 0.00534 13600.00 10000.00 0.00 0.00622 13800.00 10000.00 0.00 0.00725 14000.00 10000.00 0.00 0.00815 14200.00 10000.00 0.00 0.00890 14400.00 10000.00 0.00 0.00978 14600.00 10000.00 0.00 0.01082 14800.00 10000.00 0.00 0.01189 15000.00 10000.00 0.00 0.01292 15200.00 10000.00 0.00 0.01402 15400.00 10000.00 0.00 0.01517 15600.00 10000.00 0.00 0.01623 15800.00 10000.00 0.00 0.01736 16000.00 10000.00 0.00 0.01870 16200.00 10000.00 0.00 0.01999 16400.00 10000.00 0.00 0.02132 16600.00 10000.00 0.00 0.02282 16800.00 10000.00 0.00 0.02422 17000.00 10000.00 0.00 0.02565 17200.00 10000.00 0.00 0.02712 17400.00 10000.00 0.00 0.02855 17600.00 10000.00 0.00 0.02993 17800.00 10000.00 0.00 0.03139 18000.00 10000.00 0.00 0.03299 18200.00 10000.00 0.00 0.03457 18400.00 10000.00 0.00 0.03620 18600.00 10000.00 0.00 0.03782 18800.00 10000.00 0.00 0.03966 19000.00 10000.00 0.00 0.04163 19200.00 10000.00 0.00 0.04337 19400.00 10000.00 0.00 0.04513 19600.00 10000.00 0.00 0.04711 19800.00 10000.00 0.00 0.04930 20000.00 10000.00 0.00 0.05164 20200.00 10000.00 0.00 0.05398 20400.00 10000.00 0.00 0.05584 20600.00 10000.00 0.00 0.05683 20800.00 10000.00 0.00 0.05728 21000.00 10000.00 0.00 0.05766 21200.00 10000.00 0.00 0.05784 21400.00 10000.00 0.00 0.05788 20800.00 10200.00 0.00 0.05810 21000.00 10200.00 0.00 0.05845 21200.00 10200.00 0.00 0.05862 21400.00 10200.00 0.00 0.05866 20800.00 10400.00 0.00 0.05890 21000.00 10400.00 0.00 0.05926 21200.00 10400.00 0.00 0.05944 21400.00 10400.00 0.00 0.05946 20800.00 10600.00 0.00 0.05977 21000.00 10600.00 0.00 0.06017 21200.00 10600.00 0.00 0.06032 20800.00 10800.00 0.00 0.06076 21000.00 10800.00 0.00 0.06113 21200.00 10800.00 0.00 0.06121 20800.00 11000.00 0.00 0.06170 21000.00 11000.00 0.00 0.06198 21200.00 11000.00 0.00 0.06201 20600.00 11200.00 0.00 0.06204 20800.00 11200.00 0.00 0.06253 21000.00 11200.00 0.00 0.06276 21200.00 11200.00 0.00 0.06279 20600.00 11400.00 0.00 0.06294 20800.00 11400.00 0.00 0.06342 21000.00 11400.00 0.00 0.06364 21200.00 11400.00 0.00 0.06373 21400.00 11400.00 0.00 0.06373 20600.00 11600.00 0.00 0.06387 20800.00 11600.00 0.00 0.06434 21000.00 11600.00 0.00 0.06456 21200.00 11600.00 0.00 0.06466 20600.00 11800.00 0.00 0.06494 20800.00 11800.00 0.00 0.06537 21000.00 11800.00 0.00 0.06558 21200.00 11800.00 0.00 0.06567 20600.00 12000.00 0.00 0.06601 20800.00 12000.00 0.00 0.06644 21000.00 12000.00 0.00 0.06665 21200.00 12000.00 0.00 0.06675 20600.00 12200.00 0.00 0.06725 20800.00 12200.00 0.00 0.06767 21000.00 12200.00 0.00 0.06787 21200.00 12200.00 0.00 0.06792 20600.00 12400.00 0.00 0.06859 20800.00 12400.00 0.00 0.06897 21000.00 12400.00 0.00 0.06911 21200.00 12400.00 0.00 0.06912 20600.00 12600.00 0.00 0.06981 20800.00 12600.00 0.00 0.07017 21000.00 12600.00 0.00 0.07029 20600.00 12800.00 0.00 0.07121 20800.00 12800.00 0.00 0.07156 21000.00 12800.00 0.00 0.07164 20600.00 13000.00 0.00 0.07251 20800.00 13000.00 0.00 0.07278 20400.00 13200.00 0.00 0.07279 20600.00 13200.00 0.00 0.07365 20800.00 13200.00 0.00 0.07385 20400.00 13400.00 0.00 0.07393 20600.00 13400.00 0.00 0.07478 20800.00 13400.00 0.00 0.07499 20400.00 13600.00 0.00 0.07505 20600.00 13600.00 0.00 0.07590 20800.00 13600.00 0.00 0.07612 20400.00 13800.00 0.00 0.07620 20600.00 13800.00 0.00 0.07704 20800.00 13800.00 0.00 0.07727 20400.00 14000.00 0.00 0.07748 20600.00 14000.00 0.00 0.07835 20800.00 14000.00 0.00 0.07859 20400.00 14200.00 0.00 0.07882 20600.00 14200.00 0.00 0.07974 20800.00 14200.00 0.00 0.07999 20400.00 14400.00 0.00 0.08013 20600.00 14400.00 0.00 0.08107 20800.00 14400.00 0.00 0.08129 20400.00 14600.00 0.00 0.08176 20600.00 14600.00 0.00 0.08269 20800.00 14600.00 0.00 0.08284 20400.00 14800.00 0.00 0.08345 20600.00 14800.00 0.00 0.08434 20800.00 14800.00 0.00 0.08442 20400.00 15000.00 0.00 0.08520 20600.00 15000.00 0.00 0.08602 20800.00 15000.00 0.00 0.08605 20400.00 15200.00 0.00 0.08689 20600.00 15200.00 0.00 0.08767 20400.00 15400.00 0.00 0.08842 20600.00 15400.00 0.00 0.08915 20400.00 15600.00 0.00 0.08999 20600.00 15600.00 0.00 0.09069 20400.00 15800.00 0.00 0.09151 20600.00 15800.00 0.00 0.09219 20400.00 16000.00 0.00 0.09320 20600.00 16000.00 0.00 0.09388 20400.00 16200.00 0.00 0.09502 20600.00 16200.00 0.00 0.09568 20400.00 16400.00 0.00 0.09689 20600.00 16400.00 0.00 0.09753 20400.00 16600.00 0.00 0.09868 20600.00 16600.00 0.00 0.09929 20400.00 16800.00 0.00 0.10059 20600.00 16800.00 0.00 0.10114 20400.00 17000.00 0.00 0.10256 20600.00 17000.00 0.00 0.10304 20400.00 17200.00 0.00 0.10451 20600.00 17200.00 0.00 0.10497 20400.00 17400.00 0.00 0.10668 20600.00 17400.00 0.00 0.10708 20400.00 17600.00 0.00 0.10861 20600.00 17600.00 0.00 0.10888 20400.00 17800.00 0.00 0.11054 20600.00 17800.00 0.00 0.11072 20200.00 18000.00 0.00 0.11084 20400.00 18000.00 0.00 0.11257 20600.00 18000.00 0.00 0.11269 20200.00 18200.00 0.00 0.11308 20400.00 18200.00 0.00 0.11474 20600.00 18200.00 0.00 0.11477 20200.00 18400.00 0.00 0.11525 20400.00 18400.00 0.00 0.11683 20200.00 18600.00 0.00 0.11734 20400.00 18600.00 0.00 0.11889 20200.00 18800.00 0.00 0.11962 20400.00 18800.00 0.00 0.12116 20200.00 19000.00 0.00 0.12192 20400.00 19000.00 0.00 0.12343 20200.00 19200.00 0.00 0.12454 20400.00 19200.00 0.00 0.12591 20200.00 19400.00 0.00 0.12695 20400.00 19400.00 0.00 0.12814 20200.00 19600.00 0.00 0.12953 20400.00 19600.00 0.00 0.13049 20200.00 19800.00 0.00 0.13185 20400.00 19800.00 0.00 0.13249 20200.00 20000.00 0.00 0.13382 20400.00 20000.00 0.00 0.13402 20200.00 20200.00 0.00 0.13579 20200.00 20400.00 0.00 0.13592 20200.00 20400.00 5.00 0.13604 20200.00 20400.00 10.00 0.13615 20200.00 20400.00 15.00 0.13625 20200.00 20400.00 20.00 0.13633 20200.00 20400.00 25.00 0.13639 20200.00 20400.00 30.00 0.13642 20200.00 20400.00 35.00 0.13643 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20202.94 20364.16 35.00 0.13645 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/396.ctf NX, NY, NZ: 512 512 1 MODE : real