CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/394.mrc Output diagnostic file name Micrographs_0601/394.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/394.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2120.000 7728.000 Mean, RMS : 4486.566 215.5001 TITLE 1: emi2mrc: 394.emi Sun Jan 05 23:41:12 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 52.987, Y 574.403, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04837 10200.00 10000.00 0.00 0.05111 10400.00 10000.00 0.00 0.05359 10600.00 10000.00 0.00 0.05627 10800.00 10000.00 0.00 0.05886 11000.00 10000.00 0.00 0.06185 11200.00 10000.00 0.00 0.06530 11400.00 10000.00 0.00 0.06879 11600.00 10000.00 0.00 0.07248 11800.00 10000.00 0.00 0.07623 12000.00 10000.00 0.00 0.08009 12200.00 10000.00 0.00 0.08385 12400.00 10000.00 0.00 0.08792 12600.00 10000.00 0.00 0.09207 12800.00 10000.00 0.00 0.09628 13000.00 10000.00 0.00 0.10063 13200.00 10000.00 0.00 0.10410 13400.00 10000.00 0.00 0.10558 13200.00 10200.00 0.00 0.10717 13400.00 10200.00 0.00 0.10861 13200.00 10400.00 0.00 0.11042 13400.00 10400.00 0.00 0.11185 13200.00 10600.00 0.00 0.11407 13400.00 10600.00 0.00 0.11549 13200.00 10800.00 0.00 0.11773 13400.00 10800.00 0.00 0.11908 13200.00 11000.00 0.00 0.12160 13400.00 11000.00 0.00 0.12280 13200.00 11200.00 0.00 0.12528 13400.00 11200.00 0.00 0.12629 13200.00 11400.00 0.00 0.12917 13400.00 11400.00 0.00 0.13005 13200.00 11600.00 0.00 0.13302 13400.00 11600.00 0.00 0.13381 13200.00 11800.00 0.00 0.13717 13400.00 11800.00 0.00 0.13781 13000.00 12000.00 0.00 0.13816 13200.00 12000.00 0.00 0.14153 13400.00 12000.00 0.00 0.14195 13000.00 12200.00 0.00 0.14273 13200.00 12200.00 0.00 0.14603 13400.00 12200.00 0.00 0.14616 13000.00 12400.00 0.00 0.14779 13200.00 12400.00 0.00 0.15092 13000.00 12600.00 0.00 0.15260 13200.00 12600.00 0.00 0.15549 13000.00 12800.00 0.00 0.15782 13200.00 12800.00 0.00 0.16014 13000.00 13000.00 0.00 0.16190 13200.00 13000.00 0.00 0.16345 13000.00 13200.00 0.00 0.16439 13200.00 13200.00 0.00 0.16499 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13095.67 13235.41 22.68 0.16550 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/394.ctf NX, NY, NZ: 512 512 1 MODE : real