CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/393.mrc Output diagnostic file name Micrographs_0601/393.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/393.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2326.000 7638.000 Mean, RMS : 4484.881 216.8221 TITLE 1: emi2mrc: 393.emi Sun Jan 05 23:40:31 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 50.507, Y 574.715, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03833 10200.00 10000.00 0.00 0.04013 10400.00 10000.00 0.00 0.04171 10600.00 10000.00 0.00 0.04374 10800.00 10000.00 0.00 0.04589 11000.00 10000.00 0.00 0.04832 11200.00 10000.00 0.00 0.05092 11400.00 10000.00 0.00 0.05360 11600.00 10000.00 0.00 0.05673 11800.00 10000.00 0.00 0.05993 12000.00 10000.00 0.00 0.06318 12200.00 10000.00 0.00 0.06649 12400.00 10000.00 0.00 0.06995 12600.00 10000.00 0.00 0.07330 12800.00 10000.00 0.00 0.07677 13000.00 10000.00 0.00 0.08031 13200.00 10000.00 0.00 0.08390 13400.00 10000.00 0.00 0.08776 13600.00 10000.00 0.00 0.09145 13800.00 10000.00 0.00 0.09545 14000.00 10000.00 0.00 0.09882 14200.00 10000.00 0.00 0.10021 14000.00 10200.00 0.00 0.10134 14200.00 10200.00 0.00 0.10268 14000.00 10400.00 0.00 0.10399 14200.00 10400.00 0.00 0.10525 14000.00 10600.00 0.00 0.10657 14200.00 10600.00 0.00 0.10774 14000.00 10800.00 0.00 0.10913 14200.00 10800.00 0.00 0.11026 14000.00 11000.00 0.00 0.11210 14200.00 11000.00 0.00 0.11319 14000.00 11200.00 0.00 0.11496 14200.00 11200.00 0.00 0.11599 14000.00 11400.00 0.00 0.11826 14200.00 11400.00 0.00 0.11920 14000.00 11600.00 0.00 0.12158 14200.00 11600.00 0.00 0.12241 14000.00 11800.00 0.00 0.12526 14200.00 11800.00 0.00 0.12597 14000.00 12000.00 0.00 0.12909 14200.00 12000.00 0.00 0.12969 14000.00 12200.00 0.00 0.13269 14200.00 12200.00 0.00 0.13315 13800.00 12400.00 0.00 0.13327 14000.00 12400.00 0.00 0.13640 14200.00 12400.00 0.00 0.13669 13800.00 12600.00 0.00 0.13682 14000.00 12600.00 0.00 0.13994 14200.00 12600.00 0.00 0.14004 13800.00 12800.00 0.00 0.14087 14000.00 12800.00 0.00 0.14393 13800.00 13000.00 0.00 0.14461 14000.00 13000.00 0.00 0.14751 13800.00 13200.00 0.00 0.14859 14000.00 13200.00 0.00 0.15121 13800.00 13400.00 0.00 0.15232 14000.00 13400.00 0.00 0.15458 13800.00 13600.00 0.00 0.15601 14000.00 13600.00 0.00 0.15771 13800.00 13800.00 0.00 0.16023 14000.00 13800.00 0.00 0.16072 13800.00 14000.00 0.00 0.16168 13800.00 14000.00 5.00 0.16184 13800.00 14000.00 10.00 0.16198 13800.00 14000.00 15.00 0.16210 13800.00 14000.00 20.00 0.16220 13800.00 14000.00 25.00 0.16228 13800.00 14000.00 30.00 0.16232 13800.00 14000.00 35.00 0.16234 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13839.36 14023.93 32.33 0.16251 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/393.ctf NX, NY, NZ: 512 512 1 MODE : real