CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/392.mrc Output diagnostic file name Micrographs_0601/392.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/392.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2302.000 7494.000 Mean, RMS : 4484.322 217.3210 TITLE 1: emi2mrc: 392.emi Sun Jan 05 23:39:18 2014 TITLE 2: kV 300, Mag 78000x, Def 18210, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 45.408, Y 575.359, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01538 10200.00 10000.00 0.00 0.01625 10400.00 10000.00 0.00 0.01728 10600.00 10000.00 0.00 0.01851 10800.00 10000.00 0.00 0.01963 11000.00 10000.00 0.00 0.02068 11200.00 10000.00 0.00 0.02174 11400.00 10000.00 0.00 0.02288 11600.00 10000.00 0.00 0.02409 11800.00 10000.00 0.00 0.02531 12000.00 10000.00 0.00 0.02663 12200.00 10000.00 0.00 0.02806 12400.00 10000.00 0.00 0.02974 12600.00 10000.00 0.00 0.03148 12800.00 10000.00 0.00 0.03337 13000.00 10000.00 0.00 0.03530 13200.00 10000.00 0.00 0.03710 13400.00 10000.00 0.00 0.03908 13600.00 10000.00 0.00 0.04122 13800.00 10000.00 0.00 0.04344 14000.00 10000.00 0.00 0.04570 14200.00 10000.00 0.00 0.04793 14400.00 10000.00 0.00 0.04999 14600.00 10000.00 0.00 0.05223 14800.00 10000.00 0.00 0.05490 15000.00 10000.00 0.00 0.05781 15200.00 10000.00 0.00 0.06088 15400.00 10000.00 0.00 0.06407 15600.00 10000.00 0.00 0.06754 15800.00 10000.00 0.00 0.07092 16000.00 10000.00 0.00 0.07433 16200.00 10000.00 0.00 0.07699 16400.00 10000.00 0.00 0.07795 16600.00 10000.00 0.00 0.07832 16800.00 10000.00 0.00 0.07860 16400.00 10200.00 0.00 0.07931 16600.00 10200.00 0.00 0.07969 16800.00 10200.00 0.00 0.07991 16400.00 10400.00 0.00 0.08078 16600.00 10400.00 0.00 0.08116 16800.00 10400.00 0.00 0.08132 16200.00 10600.00 0.00 0.08141 16400.00 10600.00 0.00 0.08242 16600.00 10600.00 0.00 0.08277 16800.00 10600.00 0.00 0.08287 16200.00 10800.00 0.00 0.08310 16400.00 10800.00 0.00 0.08410 16600.00 10800.00 0.00 0.08439 16800.00 10800.00 0.00 0.08446 16200.00 11000.00 0.00 0.08490 16400.00 11000.00 0.00 0.08585 16600.00 11000.00 0.00 0.08611 16800.00 11000.00 0.00 0.08614 16200.00 11200.00 0.00 0.08663 16400.00 11200.00 0.00 0.08753 16600.00 11200.00 0.00 0.08774 16800.00 11200.00 0.00 0.08775 16200.00 11400.00 0.00 0.08864 16400.00 11400.00 0.00 0.08950 16600.00 11400.00 0.00 0.08971 16200.00 11600.00 0.00 0.09067 16400.00 11600.00 0.00 0.09156 16600.00 11600.00 0.00 0.09178 16200.00 11800.00 0.00 0.09286 16400.00 11800.00 0.00 0.09375 16600.00 11800.00 0.00 0.09389 16200.00 12000.00 0.00 0.09512 16400.00 12000.00 0.00 0.09593 16600.00 12000.00 0.00 0.09595 16200.00 12200.00 0.00 0.09748 16400.00 12200.00 0.00 0.09824 16600.00 12200.00 0.00 0.09824 16200.00 12400.00 0.00 0.10017 16400.00 12400.00 0.00 0.10090 16200.00 12600.00 0.00 0.10275 16400.00 12600.00 0.00 0.10341 16200.00 12800.00 0.00 0.10542 16400.00 12800.00 0.00 0.10602 16200.00 13000.00 0.00 0.10803 16400.00 13000.00 0.00 0.10858 16200.00 13200.00 0.00 0.11090 16400.00 13200.00 0.00 0.11138 16200.00 13400.00 0.00 0.11392 16400.00 13400.00 0.00 0.11433 16200.00 13600.00 0.00 0.11700 16400.00 13600.00 0.00 0.11729 16000.00 13800.00 0.00 0.11739 16200.00 13800.00 0.00 0.12004 16400.00 13800.00 0.00 0.12017 16000.00 14000.00 0.00 0.12033 16200.00 14000.00 0.00 0.12295 16000.00 14200.00 0.00 0.12365 16200.00 14200.00 0.00 0.12622 16000.00 14400.00 0.00 0.12683 16200.00 14400.00 0.00 0.12931 16000.00 14600.00 0.00 0.13020 16200.00 14600.00 0.00 0.13266 16000.00 14800.00 0.00 0.13370 16200.00 14800.00 0.00 0.13618 16000.00 15000.00 0.00 0.13753 16200.00 15000.00 0.00 0.13995 16000.00 15200.00 0.00 0.14155 16200.00 15200.00 0.00 0.14379 16000.00 15400.00 0.00 0.14572 16200.00 15400.00 0.00 0.14771 16000.00 15600.00 0.00 0.15015 16200.00 15600.00 0.00 0.15175 16000.00 15800.00 0.00 0.15431 16200.00 15800.00 0.00 0.15539 16000.00 16000.00 0.00 0.15914 16000.00 16200.00 0.00 0.16033 16000.00 16200.00 5.00 0.16047 16000.00 16200.00 10.00 0.16059 16000.00 16200.00 15.00 0.16068 16000.00 16200.00 20.00 0.16074 16000.00 16200.00 25.00 0.16076 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16033.19 16139.11 24.60 0.16109 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/392.ctf NX, NY, NZ: 512 512 1 MODE : real