CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/390.mrc Output diagnostic file name Micrographs_0601/390.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/390.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2554.000 7912.000 Mean, RMS : 4482.537 217.4609 TITLE 1: emi2mrc: 390.emi Sun Jan 05 23:37:01 2014 TITLE 2: kV 300, Mag 78000x, Def 18380, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 37.398, Y 576.284, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00530 10200.00 10000.00 0.00 0.00579 10400.00 10000.00 0.00 0.00625 10600.00 10000.00 0.00 0.00661 10800.00 10000.00 0.00 0.00713 11000.00 10000.00 0.00 0.00778 11200.00 10000.00 0.00 0.00835 11400.00 10000.00 0.00 0.00901 11600.00 10000.00 0.00 0.00960 11800.00 10000.00 0.00 0.00999 12000.00 10000.00 0.00 0.01044 12200.00 10000.00 0.00 0.01121 12400.00 10000.00 0.00 0.01218 12600.00 10000.00 0.00 0.01307 12800.00 10000.00 0.00 0.01400 13000.00 10000.00 0.00 0.01476 13200.00 10000.00 0.00 0.01552 13400.00 10000.00 0.00 0.01649 13600.00 10000.00 0.00 0.01756 13800.00 10000.00 0.00 0.01893 14000.00 10000.00 0.00 0.02035 14200.00 10000.00 0.00 0.02173 14400.00 10000.00 0.00 0.02317 14600.00 10000.00 0.00 0.02469 14800.00 10000.00 0.00 0.02641 15000.00 10000.00 0.00 0.02819 15200.00 10000.00 0.00 0.02999 15400.00 10000.00 0.00 0.03176 15600.00 10000.00 0.00 0.03354 15800.00 10000.00 0.00 0.03526 16000.00 10000.00 0.00 0.03679 16200.00 10000.00 0.00 0.03836 16400.00 10000.00 0.00 0.04001 16600.00 10000.00 0.00 0.04170 16800.00 10000.00 0.00 0.04349 17000.00 10000.00 0.00 0.04531 17200.00 10000.00 0.00 0.04696 17400.00 10000.00 0.00 0.04866 17600.00 10000.00 0.00 0.05061 17800.00 10000.00 0.00 0.05270 18000.00 10000.00 0.00 0.05475 18200.00 10000.00 0.00 0.05689 18400.00 10000.00 0.00 0.05894 18600.00 10000.00 0.00 0.06023 18800.00 10000.00 0.00 0.06090 19000.00 10000.00 0.00 0.06133 19200.00 10000.00 0.00 0.06172 19400.00 10000.00 0.00 0.06197 19600.00 10000.00 0.00 0.06206 19800.00 10000.00 0.00 0.06214 19000.00 10200.00 0.00 0.06241 19200.00 10200.00 0.00 0.06282 19400.00 10200.00 0.00 0.06307 19600.00 10200.00 0.00 0.06316 19800.00 10200.00 0.00 0.06322 19000.00 10400.00 0.00 0.06355 19200.00 10400.00 0.00 0.06395 19400.00 10400.00 0.00 0.06419 19600.00 10400.00 0.00 0.06427 19800.00 10400.00 0.00 0.06431 19000.00 10600.00 0.00 0.06479 19200.00 10600.00 0.00 0.06516 19400.00 10600.00 0.00 0.06539 19600.00 10600.00 0.00 0.06543 19000.00 10800.00 0.00 0.06584 19200.00 10800.00 0.00 0.06617 19400.00 10800.00 0.00 0.06636 18800.00 11000.00 0.00 0.06639 19000.00 11000.00 0.00 0.06681 19200.00 11000.00 0.00 0.06712 19400.00 11000.00 0.00 0.06726 18800.00 11200.00 0.00 0.06734 19000.00 11200.00 0.00 0.06773 19200.00 11200.00 0.00 0.06801 19400.00 11200.00 0.00 0.06811 18800.00 11400.00 0.00 0.06831 19000.00 11400.00 0.00 0.06866 19200.00 11400.00 0.00 0.06891 19400.00 11400.00 0.00 0.06899 18800.00 11600.00 0.00 0.06935 19000.00 11600.00 0.00 0.06967 19200.00 11600.00 0.00 0.06991 19400.00 11600.00 0.00 0.07001 18800.00 11800.00 0.00 0.07056 19000.00 11800.00 0.00 0.07086 19200.00 11800.00 0.00 0.07110 19400.00 11800.00 0.00 0.07121 19800.00 11800.00 0.00 0.07122 18800.00 12000.00 0.00 0.07181 19000.00 12000.00 0.00 0.07211 19200.00 12000.00 0.00 0.07239 19400.00 12000.00 0.00 0.07254 19600.00 12000.00 0.00 0.07258 19800.00 12000.00 0.00 0.07262 18800.00 12200.00 0.00 0.07323 19000.00 12200.00 0.00 0.07356 19200.00 12200.00 0.00 0.07388 19400.00 12200.00 0.00 0.07407 19600.00 12200.00 0.00 0.07413 19800.00 12200.00 0.00 0.07415 18800.00 12400.00 0.00 0.07480 19000.00 12400.00 0.00 0.07516 19200.00 12400.00 0.00 0.07550 19400.00 12400.00 0.00 0.07569 19600.00 12400.00 0.00 0.07572 18800.00 12600.00 0.00 0.07627 19000.00 12600.00 0.00 0.07664 19200.00 12600.00 0.00 0.07696 19400.00 12600.00 0.00 0.07711 18800.00 12800.00 0.00 0.07783 19000.00 12800.00 0.00 0.07815 19200.00 12800.00 0.00 0.07844 19400.00 12800.00 0.00 0.07857 18600.00 13000.00 0.00 0.07867 18800.00 13000.00 0.00 0.07938 19000.00 13000.00 0.00 0.07971 19200.00 13000.00 0.00 0.08001 19400.00 13000.00 0.00 0.08014 18600.00 13200.00 0.00 0.08039 18800.00 13200.00 0.00 0.08112 19000.00 13200.00 0.00 0.08146 19200.00 13200.00 0.00 0.08174 19400.00 13200.00 0.00 0.08183 18600.00 13400.00 0.00 0.08224 18800.00 13400.00 0.00 0.08292 19000.00 13400.00 0.00 0.08320 19200.00 13400.00 0.00 0.08342 19400.00 13400.00 0.00 0.08344 18600.00 13600.00 0.00 0.08413 18800.00 13600.00 0.00 0.08475 19000.00 13600.00 0.00 0.08500 19200.00 13600.00 0.00 0.08520 19400.00 13600.00 0.00 0.08524 18600.00 13800.00 0.00 0.08597 18800.00 13800.00 0.00 0.08658 19000.00 13800.00 0.00 0.08685 19200.00 13800.00 0.00 0.08710 19400.00 13800.00 0.00 0.08716 18600.00 14000.00 0.00 0.08781 18800.00 14000.00 0.00 0.08844 19000.00 14000.00 0.00 0.08876 19200.00 14000.00 0.00 0.08901 19400.00 14000.00 0.00 0.08901 18600.00 14200.00 0.00 0.08988 18800.00 14200.00 0.00 0.09054 19000.00 14200.00 0.00 0.09087 19200.00 14200.00 0.00 0.09106 18600.00 14400.00 0.00 0.09186 18800.00 14400.00 0.00 0.09251 19000.00 14400.00 0.00 0.09280 19200.00 14400.00 0.00 0.09287 18600.00 14600.00 0.00 0.09388 18800.00 14600.00 0.00 0.09450 19000.00 14600.00 0.00 0.09469 18600.00 14800.00 0.00 0.09601 18800.00 14800.00 0.00 0.09660 19000.00 14800.00 0.00 0.09673 18400.00 15000.00 0.00 0.09696 18600.00 15000.00 0.00 0.09830 18800.00 15000.00 0.00 0.09885 19000.00 15000.00 0.00 0.09889 18400.00 15200.00 0.00 0.09920 18600.00 15200.00 0.00 0.10051 18800.00 15200.00 0.00 0.10097 18400.00 15400.00 0.00 0.10154 18600.00 15400.00 0.00 0.10279 18800.00 15400.00 0.00 0.10319 18400.00 15600.00 0.00 0.10398 18600.00 15600.00 0.00 0.10518 18800.00 15600.00 0.00 0.10555 18400.00 15800.00 0.00 0.10624 18600.00 15800.00 0.00 0.10742 18800.00 15800.00 0.00 0.10779 18400.00 16000.00 0.00 0.10875 18600.00 16000.00 0.00 0.10990 18800.00 16000.00 0.00 0.11022 18400.00 16200.00 0.00 0.11106 18600.00 16200.00 0.00 0.11217 18800.00 16200.00 0.00 0.11242 18400.00 16400.00 0.00 0.11344 18600.00 16400.00 0.00 0.11454 18800.00 16400.00 0.00 0.11474 18400.00 16600.00 0.00 0.11596 18600.00 16600.00 0.00 0.11706 18800.00 16600.00 0.00 0.11720 18400.00 16800.00 0.00 0.11871 18600.00 16800.00 0.00 0.11978 18800.00 16800.00 0.00 0.11981 18400.00 17000.00 0.00 0.12173 18600.00 17000.00 0.00 0.12270 18400.00 17200.00 0.00 0.12470 18600.00 17200.00 0.00 0.12553 18200.00 17400.00 0.00 0.12562 18400.00 17400.00 0.00 0.12787 18600.00 17400.00 0.00 0.12851 18200.00 17600.00 0.00 0.12870 18400.00 17600.00 0.00 0.13087 18600.00 17600.00 0.00 0.13134 18200.00 17800.00 0.00 0.13222 18400.00 17800.00 0.00 0.13429 18600.00 17800.00 0.00 0.13456 18200.00 18000.00 0.00 0.13548 18400.00 18000.00 0.00 0.13755 18600.00 18000.00 0.00 0.13755 18200.00 18200.00 0.00 0.13878 18400.00 18200.00 0.00 0.14072 18200.00 18400.00 0.00 0.14146 18400.00 18400.00 0.00 0.14271 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 18355.03 18455.73 35.83 0.14288 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/390.ctf NX, NY, NZ: 512 512 1 MODE : real