CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/388.mrc Output diagnostic file name Micrographs_0601/388.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/388.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2492.000 8036.000 Mean, RMS : 4510.067 221.7886 TITLE 1: emi2mrc: 388.emi Sun Jan 05 23:35:25 2014 TITLE 2: kV 300, Mag 78000x, Def 18710, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 39.799, Y 578.696, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01859 14800.00 10000.00 0.00 -0.01831 15000.00 10000.00 0.00 -0.01787 15200.00 10000.00 0.00 -0.01759 15400.00 10000.00 0.00 -0.01727 15600.00 10000.00 0.00 -0.01702 15800.00 10000.00 0.00 -0.01682 16000.00 10000.00 0.00 -0.01643 16200.00 10000.00 0.00 -0.01594 16400.00 10000.00 0.00 -0.01552 16600.00 10000.00 0.00 -0.01517 16800.00 10000.00 0.00 -0.01471 17000.00 10000.00 0.00 -0.01423 17200.00 10000.00 0.00 -0.01379 17400.00 10000.00 0.00 -0.01337 17600.00 10000.00 0.00 -0.01286 17800.00 10000.00 0.00 -0.01212 18000.00 10000.00 0.00 -0.01133 18200.00 10000.00 0.00 -0.01069 18400.00 10000.00 0.00 -0.01017 18600.00 10000.00 0.00 -0.00968 18800.00 10000.00 0.00 -0.00914 19000.00 10000.00 0.00 -0.00858 19200.00 10000.00 0.00 -0.00798 19400.00 10000.00 0.00 -0.00743 19600.00 10000.00 0.00 -0.00684 19800.00 10000.00 0.00 -0.00606 20000.00 10000.00 0.00 -0.00517 20200.00 10000.00 0.00 -0.00418 20400.00 10000.00 0.00 -0.00320 20600.00 10000.00 0.00 -0.00234 20800.00 10000.00 0.00 -0.00146 21000.00 10000.00 0.00 -0.00071 21200.00 10000.00 0.00 0.00004 21400.00 10000.00 0.00 0.00092 21600.00 10000.00 0.00 0.00179 21800.00 10000.00 0.00 0.00272 22000.00 10000.00 0.00 0.00366 22200.00 10000.00 0.00 0.00455 22400.00 10000.00 0.00 0.00546 22600.00 10000.00 0.00 0.00625 22800.00 10000.00 0.00 0.00708 23000.00 10000.00 0.00 0.00811 23200.00 10000.00 0.00 0.00917 23400.00 10000.00 0.00 0.01032 23600.00 10000.00 0.00 0.01164 23800.00 10000.00 0.00 0.01299 24000.00 10000.00 0.00 0.01436 24200.00 10000.00 0.00 0.01574 24400.00 10000.00 0.00 0.01713 24600.00 10000.00 0.00 0.01863 24800.00 10000.00 0.00 0.02020 25000.00 10000.00 0.00 0.02183 25200.00 10000.00 0.00 0.02357 25400.00 10000.00 0.00 0.02536 25600.00 10000.00 0.00 0.02686 25800.00 10000.00 0.00 0.02756 26000.00 10000.00 0.00 0.02769 26200.00 10000.00 0.00 0.02776 25800.00 10200.00 0.00 0.02783 26000.00 10200.00 0.00 0.02795 26200.00 10200.00 0.00 0.02800 25800.00 10400.00 0.00 0.02813 26000.00 10400.00 0.00 0.02822 26200.00 10400.00 0.00 0.02826 25800.00 10600.00 0.00 0.02846 26000.00 10600.00 0.00 0.02852 26200.00 10600.00 0.00 0.02858 25800.00 10800.00 0.00 0.02885 26000.00 10800.00 0.00 0.02892 26200.00 10800.00 0.00 0.02901 25800.00 11000.00 0.00 0.02911 26000.00 11000.00 0.00 0.02921 26200.00 11000.00 0.00 0.02932 26200.00 11200.00 0.00 0.02937 26000.00 11400.00 0.00 0.02951 26200.00 11400.00 0.00 0.02957 25800.00 11600.00 0.00 0.02966 26000.00 11600.00 0.00 0.02985 26200.00 11600.00 0.00 0.02990 25800.00 11800.00 0.00 0.03010 26000.00 11800.00 0.00 0.03027 26200.00 11800.00 0.00 0.03031 25800.00 12000.00 0.00 0.03056 26000.00 12000.00 0.00 0.03070 26200.00 12000.00 0.00 0.03071 25800.00 12200.00 0.00 0.03078 26000.00 12200.00 0.00 0.03086 25800.00 12400.00 0.00 0.03095 26000.00 12400.00 0.00 0.03099 26200.00 12400.00 0.00 0.03100 25800.00 12600.00 0.00 0.03113 26000.00 12600.00 0.00 0.03119 26200.00 12600.00 0.00 0.03124 25800.00 12800.00 0.00 0.03135 26000.00 12800.00 0.00 0.03149 26200.00 12800.00 0.00 0.03158 25800.00 13000.00 0.00 0.03171 26000.00 13000.00 0.00 0.03194 26200.00 13000.00 0.00 0.03204 25800.00 13200.00 0.00 0.03238 26000.00 13200.00 0.00 0.03261 26200.00 13200.00 0.00 0.03262 25800.00 13400.00 0.00 0.03321 26000.00 13400.00 0.00 0.03335 25800.00 13600.00 0.00 0.03388 26000.00 13600.00 0.00 0.03399 25800.00 13800.00 0.00 0.03452 26000.00 13800.00 0.00 0.03467 26200.00 13800.00 0.00 0.03469 25800.00 14000.00 0.00 0.03524 26000.00 14000.00 0.00 0.03540 26200.00 14000.00 0.00 0.03543 25800.00 14200.00 0.00 0.03596 26000.00 14200.00 0.00 0.03612 26200.00 14200.00 0.00 0.03615 25800.00 14400.00 0.00 0.03670 26000.00 14400.00 0.00 0.03688 26200.00 14400.00 0.00 0.03692 25800.00 14600.00 0.00 0.03753 26000.00 14600.00 0.00 0.03772 26200.00 14600.00 0.00 0.03777 25600.00 14800.00 0.00 0.03782 25800.00 14800.00 0.00 0.03852 26000.00 14800.00 0.00 0.03869 26200.00 14800.00 0.00 0.03871 25600.00 15000.00 0.00 0.03894 25800.00 15000.00 0.00 0.03959 26000.00 15000.00 0.00 0.03972 25600.00 15200.00 0.00 0.03991 25800.00 15200.00 0.00 0.04052 26000.00 15200.00 0.00 0.04061 25600.00 15400.00 0.00 0.04088 25800.00 15400.00 0.00 0.04145 26000.00 15400.00 0.00 0.04150 25600.00 15600.00 0.00 0.04178 25800.00 15600.00 0.00 0.04230 26000.00 15600.00 0.00 0.04231 25600.00 15800.00 0.00 0.04241 25800.00 15800.00 0.00 0.04291 26000.00 15800.00 0.00 0.04292 25600.00 16000.00 0.00 0.04308 25800.00 16000.00 0.00 0.04359 26000.00 16000.00 0.00 0.04363 25600.00 16200.00 0.00 0.04394 25800.00 16200.00 0.00 0.04447 26000.00 16200.00 0.00 0.04453 25600.00 16400.00 0.00 0.04496 25800.00 16400.00 0.00 0.04545 26000.00 16400.00 0.00 0.04550 25600.00 16600.00 0.00 0.04590 25800.00 16600.00 0.00 0.04636 26000.00 16600.00 0.00 0.04639 25600.00 16800.00 0.00 0.04677 25800.00 16800.00 0.00 0.04723 26000.00 16800.00 0.00 0.04730 25600.00 17000.00 0.00 0.04774 25800.00 17000.00 0.00 0.04825 26000.00 17000.00 0.00 0.04837 25600.00 17200.00 0.00 0.04875 25800.00 17200.00 0.00 0.04930 26000.00 17200.00 0.00 0.04943 25600.00 17400.00 0.00 0.04980 25800.00 17400.00 0.00 0.05038 26000.00 17400.00 0.00 0.05047 25600.00 17600.00 0.00 0.05090 25800.00 17600.00 0.00 0.05145 26000.00 17600.00 0.00 0.05148 25600.00 17800.00 0.00 0.05189 25800.00 17800.00 0.00 0.05241 25600.00 18000.00 0.00 0.05283 25800.00 18000.00 0.00 0.05335 25600.00 18200.00 0.00 0.05382 25800.00 18200.00 0.00 0.05435 25600.00 18400.00 0.00 0.05493 25800.00 18400.00 0.00 0.05547 25600.00 18600.00 0.00 0.05624 25800.00 18600.00 0.00 0.05676 25600.00 18800.00 0.00 0.05756 25800.00 18800.00 0.00 0.05806 25600.00 19000.00 0.00 0.05888 25800.00 19000.00 0.00 0.05940 25600.00 19200.00 0.00 0.06015 25800.00 19200.00 0.00 0.06067 25600.00 19400.00 0.00 0.06128 25800.00 19400.00 0.00 0.06175 25600.00 19600.00 0.00 0.06243 25800.00 19600.00 0.00 0.06288 25600.00 19800.00 0.00 0.06356 25800.00 19800.00 0.00 0.06405 25600.00 20000.00 0.00 0.06485 25800.00 20000.00 0.00 0.06537 25600.00 20200.00 0.00 0.06615 25800.00 20200.00 0.00 0.06664 25600.00 20400.00 0.00 0.06744 25800.00 20400.00 0.00 0.06791 25600.00 20600.00 0.00 0.06883 25800.00 20600.00 0.00 0.06928 25600.00 20800.00 0.00 0.07012 25800.00 20800.00 0.00 0.07058 25600.00 21000.00 0.00 0.07162 25800.00 21000.00 0.00 0.07207 25600.00 21200.00 0.00 0.07306 25800.00 21200.00 0.00 0.07350 25600.00 21400.00 0.00 0.07456 25800.00 21400.00 0.00 0.07500 25600.00 21600.00 0.00 0.07624 25800.00 21600.00 0.00 0.07666 25600.00 21800.00 0.00 0.07791 25800.00 21800.00 0.00 0.07826 25600.00 22000.00 0.00 0.07962 25800.00 22000.00 0.00 0.07991 25600.00 22200.00 0.00 0.08126 25800.00 22200.00 0.00 0.08149 25600.00 22400.00 0.00 0.08295 25800.00 22400.00 0.00 0.08309 25600.00 22600.00 0.00 0.08453 25800.00 22600.00 0.00 0.08459 25600.00 22800.00 0.00 0.08628 25400.00 23000.00 0.00 0.08653 25600.00 23000.00 0.00 0.08820 25400.00 23200.00 0.00 0.08863 25600.00 23200.00 0.00 0.09029 25400.00 23400.00 0.00 0.09099 25600.00 23400.00 0.00 0.09264 25400.00 23600.00 0.00 0.09341 25600.00 23600.00 0.00 0.09505 25400.00 23800.00 0.00 0.09612 25600.00 23800.00 0.00 0.09770 25400.00 24000.00 0.00 0.09895 25600.00 24000.00 0.00 0.10041 25400.00 24200.00 0.00 0.10180 25600.00 24200.00 0.00 0.10308 25400.00 24400.00 0.00 0.10446 25600.00 24400.00 0.00 0.10551 25400.00 24600.00 0.00 0.10720 25600.00 24600.00 0.00 0.10800 25400.00 24800.00 0.00 0.11004 25600.00 24800.00 0.00 0.11055 25400.00 25000.00 0.00 0.11269 25600.00 25000.00 0.00 0.11274 25200.00 25200.00 0.00 0.11309 25400.00 25200.00 0.00 0.11493 25400.00 25400.00 0.00 0.11590 25400.00 25600.00 15.00 0.11601 25200.00 25600.00 20.00 0.11606 25400.00 25600.00 20.00 0.11617 25200.00 25600.00 25.00 0.11627 25400.00 25600.00 25.00 0.11629 25200.00 25600.00 30.00 0.11643 25200.00 25600.00 35.00 0.11653 25200.00 25600.00 40.00 0.11657 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 25289.70 25593.62 40.00 0.11689 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/388.ctf NX, NY, NZ: 512 512 1 MODE : real