CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/387.mrc Output diagnostic file name Micrographs_0601/387.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/387.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2502.000 7988.000 Mean, RMS : 4508.976 221.5175 TITLE 1: emi2mrc: 387.emi Sun Jan 05 23:34:42 2014 TITLE 2: kV 300, Mag 78000x, Def 18710, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 42.666, Y 578.361, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01640 11800.00 10000.00 0.00 -0.01623 12000.00 10000.00 0.00 -0.01617 13400.00 10000.00 0.00 -0.01615 13600.00 10000.00 0.00 -0.01584 13800.00 10000.00 0.00 -0.01554 14000.00 10000.00 0.00 -0.01529 14200.00 10000.00 0.00 -0.01504 14400.00 10000.00 0.00 -0.01470 14600.00 10000.00 0.00 -0.01431 14800.00 10000.00 0.00 -0.01388 15000.00 10000.00 0.00 -0.01353 15200.00 10000.00 0.00 -0.01322 15400.00 10000.00 0.00 -0.01280 15600.00 10000.00 0.00 -0.01236 15800.00 10000.00 0.00 -0.01196 16000.00 10000.00 0.00 -0.01153 16200.00 10000.00 0.00 -0.01096 16400.00 10000.00 0.00 -0.01033 16600.00 10000.00 0.00 -0.00965 16800.00 10000.00 0.00 -0.00897 17000.00 10000.00 0.00 -0.00834 17200.00 10000.00 0.00 -0.00772 17400.00 10000.00 0.00 -0.00714 17600.00 10000.00 0.00 -0.00646 17800.00 10000.00 0.00 -0.00588 18000.00 10000.00 0.00 -0.00531 18200.00 10000.00 0.00 -0.00455 18400.00 10000.00 0.00 -0.00380 18600.00 10000.00 0.00 -0.00305 18800.00 10000.00 0.00 -0.00222 19000.00 10000.00 0.00 -0.00124 19200.00 10000.00 0.00 -0.00018 19400.00 10000.00 0.00 0.00087 19600.00 10000.00 0.00 0.00192 19800.00 10000.00 0.00 0.00289 20000.00 10000.00 0.00 0.00398 20200.00 10000.00 0.00 0.00518 20400.00 10000.00 0.00 0.00630 20600.00 10000.00 0.00 0.00741 20800.00 10000.00 0.00 0.00843 21000.00 10000.00 0.00 0.00954 21200.00 10000.00 0.00 0.01076 21400.00 10000.00 0.00 0.01180 21600.00 10000.00 0.00 0.01280 21800.00 10000.00 0.00 0.01387 22000.00 10000.00 0.00 0.01496 22200.00 10000.00 0.00 0.01593 22400.00 10000.00 0.00 0.01687 22600.00 10000.00 0.00 0.01812 22800.00 10000.00 0.00 0.01944 23000.00 10000.00 0.00 0.02069 23200.00 10000.00 0.00 0.02207 23400.00 10000.00 0.00 0.02358 23600.00 10000.00 0.00 0.02519 23800.00 10000.00 0.00 0.02690 24000.00 10000.00 0.00 0.02878 24200.00 10000.00 0.00 0.03068 24400.00 10000.00 0.00 0.03216 24600.00 10000.00 0.00 0.03316 24800.00 10000.00 0.00 0.03375 25000.00 10000.00 0.00 0.03388 24800.00 10200.00 0.00 0.03408 25000.00 10200.00 0.00 0.03418 24800.00 10400.00 0.00 0.03451 25000.00 10400.00 0.00 0.03454 24800.00 10600.00 0.00 0.03489 24800.00 10800.00 0.00 0.03521 24600.00 11000.00 0.00 0.03526 24800.00 11000.00 0.00 0.03562 25000.00 11000.00 0.00 0.03566 25200.00 11000.00 0.00 0.03566 24600.00 11200.00 0.00 0.03574 24800.00 11200.00 0.00 0.03613 25000.00 11200.00 0.00 0.03620 24600.00 11400.00 0.00 0.03620 24800.00 11400.00 0.00 0.03658 25000.00 11400.00 0.00 0.03662 24800.00 11600.00 0.00 0.03676 25000.00 11600.00 0.00 0.03677 24800.00 11800.00 0.00 0.03699 24600.00 12000.00 0.00 0.03699 24800.00 12000.00 0.00 0.03730 25000.00 12000.00 0.00 0.03730 24800.00 12200.00 0.00 0.03760 25000.00 12200.00 0.00 0.03761 24600.00 12400.00 0.00 0.03769 24800.00 12400.00 0.00 0.03805 25000.00 12400.00 0.00 0.03805 24600.00 12600.00 0.00 0.03818 24800.00 12600.00 0.00 0.03853 24600.00 12800.00 0.00 0.03868 24800.00 12800.00 0.00 0.03902 25400.00 12800.00 0.00 0.03904 24600.00 13000.00 0.00 0.03919 24800.00 13000.00 0.00 0.03953 25000.00 13000.00 0.00 0.03954 25200.00 13000.00 0.00 0.03957 25400.00 13000.00 0.00 0.03964 24600.00 13200.00 0.00 0.03973 24800.00 13200.00 0.00 0.04010 25000.00 13200.00 0.00 0.04015 25200.00 13200.00 0.00 0.04025 25400.00 13200.00 0.00 0.04033 24600.00 13400.00 0.00 0.04042 24800.00 13400.00 0.00 0.04081 25000.00 13400.00 0.00 0.04088 25200.00 13400.00 0.00 0.04099 25400.00 13400.00 0.00 0.04103 24600.00 13600.00 0.00 0.04124 24800.00 13600.00 0.00 0.04160 25000.00 13600.00 0.00 0.04164 25200.00 13600.00 0.00 0.04174 25400.00 13600.00 0.00 0.04175 24600.00 13800.00 0.00 0.04194 24800.00 13800.00 0.00 0.04226 25000.00 13800.00 0.00 0.04231 25200.00 13800.00 0.00 0.04244 25400.00 13800.00 0.00 0.04247 24600.00 14000.00 0.00 0.04254 24800.00 14000.00 0.00 0.04286 25000.00 14000.00 0.00 0.04296 25200.00 14000.00 0.00 0.04316 25400.00 14000.00 0.00 0.04319 24600.00 14200.00 0.00 0.04332 24800.00 14200.00 0.00 0.04366 25000.00 14200.00 0.00 0.04382 25200.00 14200.00 0.00 0.04405 24600.00 14400.00 0.00 0.04417 24800.00 14400.00 0.00 0.04455 25000.00 14400.00 0.00 0.04476 25200.00 14400.00 0.00 0.04498 24600.00 14600.00 0.00 0.04514 24800.00 14600.00 0.00 0.04556 25000.00 14600.00 0.00 0.04579 25200.00 14600.00 0.00 0.04598 24600.00 14800.00 0.00 0.04620 24800.00 14800.00 0.00 0.04664 25000.00 14800.00 0.00 0.04687 25200.00 14800.00 0.00 0.04701 24600.00 15000.00 0.00 0.04717 24800.00 15000.00 0.00 0.04764 25000.00 15000.00 0.00 0.04785 25200.00 15000.00 0.00 0.04792 24600.00 15200.00 0.00 0.04819 24800.00 15200.00 0.00 0.04868 25000.00 15200.00 0.00 0.04886 25200.00 15200.00 0.00 0.04889 24600.00 15400.00 0.00 0.04942 24800.00 15400.00 0.00 0.04990 25000.00 15400.00 0.00 0.05001 24600.00 15600.00 0.00 0.05068 24800.00 15600.00 0.00 0.05107 25000.00 15600.00 0.00 0.05110 24600.00 15800.00 0.00 0.05175 24800.00 15800.00 0.00 0.05205 24600.00 16000.00 0.00 0.05268 24800.00 16000.00 0.00 0.05293 24600.00 16200.00 0.00 0.05366 24800.00 16200.00 0.00 0.05393 24600.00 16400.00 0.00 0.05476 24800.00 16400.00 0.00 0.05498 24600.00 16600.00 0.00 0.05564 24800.00 16600.00 0.00 0.05580 24600.00 16800.00 0.00 0.05652 24800.00 16800.00 0.00 0.05668 24400.00 17000.00 0.00 0.05680 24600.00 17000.00 0.00 0.05757 24800.00 17000.00 0.00 0.05775 24400.00 17200.00 0.00 0.05784 24600.00 17200.00 0.00 0.05864 24800.00 17200.00 0.00 0.05879 24400.00 17400.00 0.00 0.05899 24600.00 17400.00 0.00 0.05975 24800.00 17400.00 0.00 0.05984 24400.00 17600.00 0.00 0.06014 24600.00 17600.00 0.00 0.06083 24800.00 17600.00 0.00 0.06087 24400.00 17800.00 0.00 0.06141 24600.00 17800.00 0.00 0.06206 24800.00 17800.00 0.00 0.06208 24400.00 18000.00 0.00 0.06274 24600.00 18000.00 0.00 0.06336 24800.00 18000.00 0.00 0.06342 24400.00 18200.00 0.00 0.06404 24600.00 18200.00 0.00 0.06466 24800.00 18200.00 0.00 0.06476 24400.00 18400.00 0.00 0.06536 24600.00 18400.00 0.00 0.06600 24800.00 18400.00 0.00 0.06611 24400.00 18600.00 0.00 0.06679 24600.00 18600.00 0.00 0.06740 24800.00 18600.00 0.00 0.06749 24400.00 18800.00 0.00 0.06825 24600.00 18800.00 0.00 0.06882 24800.00 18800.00 0.00 0.06888 24400.00 19000.00 0.00 0.06962 24600.00 19000.00 0.00 0.07019 24800.00 19000.00 0.00 0.07025 24400.00 19200.00 0.00 0.07105 24600.00 19200.00 0.00 0.07165 24800.00 19200.00 0.00 0.07171 24400.00 19400.00 0.00 0.07251 24600.00 19400.00 0.00 0.07312 24800.00 19400.00 0.00 0.07314 24400.00 19600.00 0.00 0.07399 24600.00 19600.00 0.00 0.07458 24400.00 19800.00 0.00 0.07541 24600.00 19800.00 0.00 0.07601 24400.00 20000.00 0.00 0.07690 24600.00 20000.00 0.00 0.07749 24400.00 20200.00 0.00 0.07850 24600.00 20200.00 0.00 0.07908 24400.00 20400.00 0.00 0.08020 24600.00 20400.00 0.00 0.08074 24400.00 20600.00 0.00 0.08201 24600.00 20600.00 0.00 0.08251 24400.00 20800.00 0.00 0.08375 24600.00 20800.00 0.00 0.08419 24400.00 21000.00 0.00 0.08547 24600.00 21000.00 0.00 0.08587 24400.00 21200.00 0.00 0.08727 24600.00 21200.00 0.00 0.08760 24400.00 21400.00 0.00 0.08927 24600.00 21400.00 0.00 0.08951 24400.00 21600.00 0.00 0.09109 24600.00 21600.00 0.00 0.09124 24200.00 21800.00 0.00 0.09141 24400.00 21800.00 0.00 0.09299 24600.00 21800.00 0.00 0.09307 24200.00 22000.00 0.00 0.09363 24400.00 22000.00 0.00 0.09519 24200.00 22200.00 0.00 0.09572 24400.00 22200.00 0.00 0.09722 24200.00 22400.00 0.00 0.09795 24400.00 22400.00 0.00 0.09936 24200.00 22600.00 0.00 0.10016 24400.00 22600.00 0.00 0.10149 24200.00 22800.00 0.00 0.10262 24400.00 22800.00 0.00 0.10390 24200.00 23000.00 0.00 0.10514 24400.00 23000.00 0.00 0.10631 24200.00 23200.00 0.00 0.10753 24400.00 23200.00 0.00 0.10854 24200.00 23400.00 0.00 0.11008 24400.00 23400.00 0.00 0.11094 24200.00 23600.00 0.00 0.11281 24400.00 23600.00 0.00 0.11340 24200.00 23800.00 0.00 0.11566 24400.00 23800.00 0.00 0.11581 24000.00 24000.00 0.00 0.11592 24200.00 24000.00 0.00 0.11791 24000.00 24200.00 0.00 0.11797 24200.00 24200.00 0.00 0.11945 24200.00 24400.00 20.00 0.11956 24200.00 24400.00 25.00 0.11969 24200.00 24400.00 30.00 0.11978 24200.00 24400.00 35.00 0.11983 24200.00 24400.00 40.00 0.11984 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24121.06 24358.34 40.00 0.12024 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/387.ctf NX, NY, NZ: 512 512 1 MODE : real