CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/378.mrc Output diagnostic file name Micrographs_0601/378.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/378.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2258.000 8028.000 Mean, RMS : 4463.188 224.5855 TITLE 1: emi2mrc: 378.emi Sun Jan 05 23:27:28 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.07%, Obj 88.10%, Dif 73.67% TITLE 3: X 66.673, Y 574.947, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.06641 10200.00 10000.00 0.00 0.07003 10400.00 10000.00 0.00 0.07318 10600.00 10000.00 0.00 0.07713 10800.00 10000.00 0.00 0.08112 11000.00 10000.00 0.00 0.08522 11200.00 10000.00 0.00 0.08940 11400.00 10000.00 0.00 0.09368 11600.00 10000.00 0.00 0.09826 11800.00 10000.00 0.00 0.10293 12000.00 10000.00 0.00 0.10790 12200.00 10000.00 0.00 0.11239 12400.00 10000.00 0.00 0.11622 12600.00 10000.00 0.00 0.11838 12800.00 10000.00 0.00 0.11868 12400.00 10200.00 0.00 0.11967 12600.00 10200.00 0.00 0.12178 12800.00 10200.00 0.00 0.12186 12400.00 10400.00 0.00 0.12337 12600.00 10400.00 0.00 0.12533 12400.00 10600.00 0.00 0.12729 12600.00 10600.00 0.00 0.12905 12400.00 10800.00 0.00 0.13102 12600.00 10800.00 0.00 0.13259 12400.00 11000.00 0.00 0.13502 12600.00 11000.00 0.00 0.13639 12400.00 11200.00 0.00 0.13868 12600.00 11200.00 0.00 0.13977 12400.00 11400.00 0.00 0.14239 12600.00 11400.00 0.00 0.14314 12400.00 11600.00 0.00 0.14553 12600.00 11600.00 0.00 0.14591 12200.00 11800.00 0.00 0.14591 12400.00 11800.00 0.00 0.14877 12200.00 12000.00 0.00 0.14905 12400.00 12000.00 0.00 0.15151 12400.00 12200.00 0.00 0.15330 12400.00 12400.00 0.00 0.15487 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12445.00 12378.62 -65.69 0.15498 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/378.ctf NX, NY, NZ: 512 512 1 MODE : real