CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/376.mrc Output diagnostic file name Micrographs_0601/376.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/376.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2280.000 8966.000 Mean, RMS : 4482.231 217.4801 TITLE 1: emi2mrc: 376.emi Sun Jan 05 23:26:15 2014 TITLE 2: kV 300, Mag 78000x, Def 18050, Spot 4, Int 55.07%, Obj 88.10%, Dif 73.67% TITLE 3: X 66.701, Y 577.770, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03247 10200.00 10000.00 0.00 0.03390 10400.00 10000.00 0.00 0.03509 10600.00 10000.00 0.00 0.03679 10800.00 10000.00 0.00 0.03850 11000.00 10000.00 0.00 0.04036 11200.00 10000.00 0.00 0.04234 11400.00 10000.00 0.00 0.04436 11600.00 10000.00 0.00 0.04676 11800.00 10000.00 0.00 0.04921 12000.00 10000.00 0.00 0.05173 12200.00 10000.00 0.00 0.05422 12400.00 10000.00 0.00 0.05677 12600.00 10000.00 0.00 0.05950 12800.00 10000.00 0.00 0.06253 13000.00 10000.00 0.00 0.06551 13200.00 10000.00 0.00 0.06837 13400.00 10000.00 0.00 0.07137 13600.00 10000.00 0.00 0.07420 13800.00 10000.00 0.00 0.07718 14000.00 10000.00 0.00 0.08026 14200.00 10000.00 0.00 0.08350 14400.00 10000.00 0.00 0.08678 14600.00 10000.00 0.00 0.08974 14800.00 10000.00 0.00 0.09201 15000.00 10000.00 0.00 0.09312 15200.00 10000.00 0.00 0.09346 14800.00 10200.00 0.00 0.09390 15000.00 10200.00 0.00 0.09503 15200.00 10200.00 0.00 0.09538 14800.00 10400.00 0.00 0.09608 15000.00 10400.00 0.00 0.09718 15200.00 10400.00 0.00 0.09752 14800.00 10600.00 0.00 0.09831 15000.00 10600.00 0.00 0.09940 15200.00 10600.00 0.00 0.09971 14800.00 10800.00 0.00 0.10058 15000.00 10800.00 0.00 0.10169 15200.00 10800.00 0.00 0.10198 14800.00 11000.00 0.00 0.10322 15000.00 11000.00 0.00 0.10433 15200.00 11000.00 0.00 0.10455 14800.00 11200.00 0.00 0.10571 15000.00 11200.00 0.00 0.10678 15200.00 11200.00 0.00 0.10692 14800.00 11400.00 0.00 0.10850 15000.00 11400.00 0.00 0.10955 15200.00 11400.00 0.00 0.10962 14800.00 11600.00 0.00 0.11141 15000.00 11600.00 0.00 0.11240 15200.00 11600.00 0.00 0.11242 14800.00 11800.00 0.00 0.11449 15000.00 11800.00 0.00 0.11542 14800.00 12000.00 0.00 0.11773 15000.00 12000.00 0.00 0.11857 14600.00 12200.00 0.00 0.11858 14800.00 12200.00 0.00 0.12099 15000.00 12200.00 0.00 0.12171 14600.00 12400.00 0.00 0.12211 14800.00 12400.00 0.00 0.12444 15000.00 12400.00 0.00 0.12500 14600.00 12600.00 0.00 0.12549 14800.00 12600.00 0.00 0.12767 15000.00 12600.00 0.00 0.12806 14600.00 12800.00 0.00 0.12884 14800.00 12800.00 0.00 0.13089 15000.00 12800.00 0.00 0.13115 14600.00 13000.00 0.00 0.13185 14800.00 13000.00 0.00 0.13387 15000.00 13000.00 0.00 0.13407 14600.00 13200.00 0.00 0.13527 14800.00 13200.00 0.00 0.13726 15000.00 13200.00 0.00 0.13733 14600.00 13400.00 0.00 0.13865 14800.00 13400.00 0.00 0.14053 14600.00 13600.00 0.00 0.14200 14800.00 13600.00 0.00 0.14376 14600.00 13800.00 0.00 0.14564 14800.00 13800.00 0.00 0.14726 14600.00 14000.00 0.00 0.14919 14800.00 14000.00 0.00 0.15061 14600.00 14200.00 0.00 0.15300 14800.00 14200.00 0.00 0.15413 14600.00 14400.00 0.00 0.15605 14800.00 14400.00 0.00 0.15688 14600.00 14600.00 0.00 0.15908 14800.00 14600.00 0.00 0.15915 14600.00 14800.00 0.00 0.15993 14600.00 14800.00 5.00 0.16002 14600.00 14800.00 10.00 0.16009 14600.00 14800.00 15.00 0.16015 14600.00 14800.00 20.00 0.16018 14600.00 14800.00 25.00 0.16020 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14640.98 14746.49 26.72 0.16038 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/376.ctf NX, NY, NZ: 512 512 1 MODE : real