CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/375.mrc Output diagnostic file name Micrographs_0601/375.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/375.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2146.000 8690.000 Mean, RMS : 4472.263 214.1019 TITLE 1: emi2mrc: 375.emi Sun Jan 05 23:25:25 2014 TITLE 2: kV 300, Mag 78000x, Def 17060, Spot 4, Int 55.07%, Obj 88.10%, Dif 73.67% TITLE 3: X 64.065, Y 578.095, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.07064 10200.00 10000.00 0.00 0.07427 10400.00 10000.00 0.00 0.07753 10600.00 10000.00 0.00 0.08134 10800.00 10000.00 0.00 0.08520 11000.00 10000.00 0.00 0.08953 11200.00 10000.00 0.00 0.09391 11400.00 10000.00 0.00 0.09794 11600.00 10000.00 0.00 0.10208 11800.00 10000.00 0.00 0.10648 12000.00 10000.00 0.00 0.11121 12200.00 10000.00 0.00 0.11539 12400.00 10000.00 0.00 0.11892 12600.00 10000.00 0.00 0.12062 12400.00 10200.00 0.00 0.12223 12600.00 10200.00 0.00 0.12386 12400.00 10400.00 0.00 0.12576 12600.00 10400.00 0.00 0.12727 12400.00 10600.00 0.00 0.12926 12600.00 10600.00 0.00 0.13058 12400.00 10800.00 0.00 0.13246 12600.00 10800.00 0.00 0.13360 12400.00 11000.00 0.00 0.13594 12600.00 11000.00 0.00 0.13688 12400.00 11200.00 0.00 0.13914 12600.00 11200.00 0.00 0.13989 12400.00 11400.00 0.00 0.14275 12600.00 11400.00 0.00 0.14326 12400.00 11600.00 0.00 0.14596 12600.00 11600.00 0.00 0.14613 12200.00 11800.00 0.00 0.14629 12400.00 11800.00 0.00 0.14909 12200.00 12000.00 0.00 0.14943 12400.00 12000.00 0.00 0.15196 12400.00 12200.00 0.00 0.15428 12200.00 12400.00 0.00 0.15459 12400.00 12400.00 0.00 0.15633 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 12374.34 12469.06 2.82 0.15654 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/375.ctf NX, NY, NZ: 512 512 1 MODE : real