CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/372.mrc Output diagnostic file name Micrographs_0601/372.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/372.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2226.000 10206.00 Mean, RMS : 4504.362 219.6896 TITLE 1: emi2mrc: 372.emi Sun Jan 05 23:22:41 2014 TITLE 2: kV 300, Mag 78000x, Def 17560, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 56.371, Y 578.910, Z -77.484, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00355 10200.00 10000.00 0.00 0.00373 10400.00 10000.00 0.00 0.00399 10600.00 10000.00 0.00 0.00432 10800.00 10000.00 0.00 0.00448 11000.00 10000.00 0.00 0.00464 11200.00 10000.00 0.00 0.00479 11400.00 10000.00 0.00 0.00498 11600.00 10000.00 0.00 0.00535 11800.00 10000.00 0.00 0.00568 12000.00 10000.00 0.00 0.00586 12200.00 10000.00 0.00 0.00609 12400.00 10000.00 0.00 0.00654 12600.00 10000.00 0.00 0.00695 12800.00 10000.00 0.00 0.00729 13000.00 10000.00 0.00 0.00766 13200.00 10000.00 0.00 0.00803 13400.00 10000.00 0.00 0.00849 13600.00 10000.00 0.00 0.00912 13800.00 10000.00 0.00 0.00984 14000.00 10000.00 0.00 0.01062 14200.00 10000.00 0.00 0.01142 14400.00 10000.00 0.00 0.01216 14600.00 10000.00 0.00 0.01297 14800.00 10000.00 0.00 0.01393 15000.00 10000.00 0.00 0.01494 15200.00 10000.00 0.00 0.01593 15400.00 10000.00 0.00 0.01695 15600.00 10000.00 0.00 0.01813 15800.00 10000.00 0.00 0.01930 16000.00 10000.00 0.00 0.02066 16200.00 10000.00 0.00 0.02222 16400.00 10000.00 0.00 0.02361 16600.00 10000.00 0.00 0.02502 16800.00 10000.00 0.00 0.02643 17000.00 10000.00 0.00 0.02772 17200.00 10000.00 0.00 0.02893 17400.00 10000.00 0.00 0.03022 17600.00 10000.00 0.00 0.03157 17800.00 10000.00 0.00 0.03292 18000.00 10000.00 0.00 0.03430 18200.00 10000.00 0.00 0.03564 18400.00 10000.00 0.00 0.03715 18600.00 10000.00 0.00 0.03886 18800.00 10000.00 0.00 0.04066 19000.00 10000.00 0.00 0.04243 19200.00 10000.00 0.00 0.04416 19400.00 10000.00 0.00 0.04606 19600.00 10000.00 0.00 0.04813 19800.00 10000.00 0.00 0.05035 20000.00 10000.00 0.00 0.05275 20200.00 10000.00 0.00 0.05516 20400.00 10000.00 0.00 0.05732 20600.00 10000.00 0.00 0.05888 20800.00 10000.00 0.00 0.05957 21000.00 10000.00 0.00 0.05984 21200.00 10000.00 0.00 0.06012 21400.00 10000.00 0.00 0.06012 21000.00 10200.00 0.00 0.06041 21200.00 10200.00 0.00 0.06066 20800.00 10400.00 0.00 0.06082 21000.00 10400.00 0.00 0.06116 21200.00 10400.00 0.00 0.06138 20800.00 10600.00 0.00 0.06172 21000.00 10600.00 0.00 0.06204 21200.00 10600.00 0.00 0.06220 20800.00 10800.00 0.00 0.06257 21000.00 10800.00 0.00 0.06286 21200.00 10800.00 0.00 0.06297 20800.00 11000.00 0.00 0.06355 21000.00 11000.00 0.00 0.06378 21200.00 11000.00 0.00 0.06387 20800.00 11200.00 0.00 0.06434 21000.00 11200.00 0.00 0.06449 21200.00 11200.00 0.00 0.06454 20800.00 11400.00 0.00 0.06505 21000.00 11400.00 0.00 0.06515 21200.00 11400.00 0.00 0.06520 20600.00 11600.00 0.00 0.06523 20800.00 11600.00 0.00 0.06586 21000.00 11600.00 0.00 0.06598 21200.00 11600.00 0.00 0.06608 20600.00 11800.00 0.00 0.06620 20800.00 11800.00 0.00 0.06684 21000.00 11800.00 0.00 0.06699 21200.00 11800.00 0.00 0.06713 20600.00 12000.00 0.00 0.06717 20800.00 12000.00 0.00 0.06783 21000.00 12000.00 0.00 0.06799 21200.00 12000.00 0.00 0.06810 20600.00 12200.00 0.00 0.06810 20800.00 12200.00 0.00 0.06874 21000.00 12200.00 0.00 0.06888 21200.00 12200.00 0.00 0.06893 20600.00 12400.00 0.00 0.06901 20800.00 12400.00 0.00 0.06963 21000.00 12400.00 0.00 0.06975 21200.00 12400.00 0.00 0.06978 20600.00 12600.00 0.00 0.06990 20800.00 12600.00 0.00 0.07052 21000.00 12600.00 0.00 0.07063 21200.00 12600.00 0.00 0.07068 20600.00 12800.00 0.00 0.07095 20800.00 12800.00 0.00 0.07157 21000.00 12800.00 0.00 0.07170 21200.00 12800.00 0.00 0.07177 20600.00 13000.00 0.00 0.07206 20800.00 13000.00 0.00 0.07270 21000.00 13000.00 0.00 0.07284 21200.00 13000.00 0.00 0.07290 20600.00 13200.00 0.00 0.07325 20800.00 13200.00 0.00 0.07389 21000.00 13200.00 0.00 0.07402 21200.00 13200.00 0.00 0.07405 20600.00 13400.00 0.00 0.07441 20800.00 13400.00 0.00 0.07504 21000.00 13400.00 0.00 0.07516 21200.00 13400.00 0.00 0.07518 20600.00 13600.00 0.00 0.07572 20800.00 13600.00 0.00 0.07634 21000.00 13600.00 0.00 0.07645 21200.00 13600.00 0.00 0.07645 20600.00 13800.00 0.00 0.07704 20800.00 13800.00 0.00 0.07762 21000.00 13800.00 0.00 0.07771 20600.00 14000.00 0.00 0.07822 20800.00 14000.00 0.00 0.07879 21000.00 14000.00 0.00 0.07887 20600.00 14200.00 0.00 0.07951 20800.00 14200.00 0.00 0.08008 21000.00 14200.00 0.00 0.08015 20600.00 14400.00 0.00 0.08064 20800.00 14400.00 0.00 0.08120 21000.00 14400.00 0.00 0.08121 20600.00 14600.00 0.00 0.08186 20800.00 14600.00 0.00 0.08240 20600.00 14800.00 0.00 0.08322 20800.00 14800.00 0.00 0.08375 20600.00 15000.00 0.00 0.08479 20800.00 15000.00 0.00 0.08535 20600.00 15200.00 0.00 0.08649 20800.00 15200.00 0.00 0.08704 20600.00 15400.00 0.00 0.08816 20800.00 15400.00 0.00 0.08869 20600.00 15600.00 0.00 0.08996 20800.00 15600.00 0.00 0.09046 20600.00 15800.00 0.00 0.09170 20800.00 15800.00 0.00 0.09213 20600.00 16000.00 0.00 0.09344 20800.00 16000.00 0.00 0.09380 20600.00 16200.00 0.00 0.09502 20800.00 16200.00 0.00 0.09537 20600.00 16400.00 0.00 0.09670 20800.00 16400.00 0.00 0.09706 20400.00 16600.00 0.00 0.09719 20600.00 16600.00 0.00 0.09855 20800.00 16600.00 0.00 0.09893 20400.00 16800.00 0.00 0.09918 20600.00 16800.00 0.00 0.10053 20800.00 16800.00 0.00 0.10087 20400.00 17000.00 0.00 0.10119 20600.00 17000.00 0.00 0.10251 20800.00 17000.00 0.00 0.10282 20400.00 17200.00 0.00 0.10316 20600.00 17200.00 0.00 0.10449 20800.00 17200.00 0.00 0.10475 20400.00 17400.00 0.00 0.10530 20600.00 17400.00 0.00 0.10662 20800.00 17400.00 0.00 0.10685 20400.00 17600.00 0.00 0.10734 20600.00 17600.00 0.00 0.10867 20800.00 17600.00 0.00 0.10886 20400.00 17800.00 0.00 0.10959 20600.00 17800.00 0.00 0.11090 20800.00 17800.00 0.00 0.11105 20400.00 18000.00 0.00 0.11187 20600.00 18000.00 0.00 0.11317 20800.00 18000.00 0.00 0.11324 20400.00 18200.00 0.00 0.11430 20600.00 18200.00 0.00 0.11554 20400.00 18400.00 0.00 0.11666 20600.00 18400.00 0.00 0.11783 20400.00 18600.00 0.00 0.11891 20600.00 18600.00 0.00 0.12000 20400.00 18800.00 0.00 0.12141 20600.00 18800.00 0.00 0.12237 20400.00 19000.00 0.00 0.12377 20600.00 19000.00 0.00 0.12460 20400.00 19200.00 0.00 0.12631 20600.00 19200.00 0.00 0.12700 20400.00 19400.00 0.00 0.12879 20600.00 19400.00 0.00 0.12930 20200.00 19600.00 0.00 0.12961 20400.00 19600.00 0.00 0.13146 20600.00 19600.00 0.00 0.13177 20200.00 19800.00 0.00 0.13239 20400.00 19800.00 0.00 0.13405 20600.00 19800.00 0.00 0.13418 20200.00 20000.00 0.00 0.13505 20400.00 20000.00 0.00 0.13647 20200.00 20200.00 0.00 0.13788 20400.00 20200.00 0.00 0.13875 20200.00 20400.00 0.00 0.13914 20400.00 20400.00 0.00 0.13952 20200.00 20600.00 15.00 0.13966 20200.00 20600.00 20.00 0.13986 20200.00 20600.00 25.00 0.14002 20200.00 20600.00 30.00 0.14013 20200.00 20600.00 35.00 0.14019 20200.00 20600.00 40.00 0.14020 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 20238.73 20545.26 36.79 0.14028 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/372.ctf NX, NY, NZ: 512 512 1 MODE : real