CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/370.mrc Output diagnostic file name Micrographs_0601/370.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/370.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2356.000 7772.000 Mean, RMS : 4495.246 215.5454 TITLE 1: emi2mrc: 370.emi Sun Jan 05 23:19:56 2014 TITLE 2: kV 300, Mag 78000x, Def 17230, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 48.543, Y 579.972, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03926 10200.00 10000.00 0.00 0.04193 10400.00 10000.00 0.00 0.04426 10600.00 10000.00 0.00 0.04687 10800.00 10000.00 0.00 0.04957 11000.00 10000.00 0.00 0.05253 11200.00 10000.00 0.00 0.05569 11400.00 10000.00 0.00 0.05895 11600.00 10000.00 0.00 0.06226 11800.00 10000.00 0.00 0.06551 12000.00 10000.00 0.00 0.06892 12200.00 10000.00 0.00 0.07233 12400.00 10000.00 0.00 0.07606 12600.00 10000.00 0.00 0.07988 12800.00 10000.00 0.00 0.08386 13000.00 10000.00 0.00 0.08787 13200.00 10000.00 0.00 0.09173 13400.00 10000.00 0.00 0.09568 13600.00 10000.00 0.00 0.09855 13800.00 10000.00 0.00 0.09964 14000.00 10000.00 0.00 0.09964 13600.00 10200.00 0.00 0.10138 13800.00 10200.00 0.00 0.10253 13600.00 10400.00 0.00 0.10441 13800.00 10400.00 0.00 0.10556 13600.00 10600.00 0.00 0.10764 13800.00 10600.00 0.00 0.10871 13600.00 10800.00 0.00 0.11107 13800.00 10800.00 0.00 0.11201 13600.00 11000.00 0.00 0.11479 13800.00 11000.00 0.00 0.11560 13600.00 11200.00 0.00 0.11825 13800.00 11200.00 0.00 0.11893 13600.00 11400.00 0.00 0.12194 13800.00 11400.00 0.00 0.12250 13400.00 11600.00 0.00 0.12262 13600.00 11600.00 0.00 0.12561 13800.00 11600.00 0.00 0.12604 13400.00 11800.00 0.00 0.12671 13600.00 11800.00 0.00 0.12966 13800.00 11800.00 0.00 0.12991 13400.00 12000.00 0.00 0.13118 13600.00 12000.00 0.00 0.13403 13800.00 12000.00 0.00 0.13405 13400.00 12200.00 0.00 0.13559 13600.00 12200.00 0.00 0.13829 13400.00 12400.00 0.00 0.14021 13600.00 12400.00 0.00 0.14273 13400.00 12600.00 0.00 0.14487 13600.00 12600.00 0.00 0.14724 13400.00 12800.00 0.00 0.15044 13600.00 12800.00 0.00 0.15249 13400.00 13000.00 0.00 0.15561 13600.00 13000.00 0.00 0.15716 13400.00 13200.00 0.00 0.16116 13600.00 13200.00 0.00 0.16175 13200.00 13400.00 0.00 0.16211 13400.00 13400.00 0.00 0.16614 13400.00 13600.00 5.00 0.16626 13400.00 13600.00 10.00 0.16642 13400.00 13600.00 15.00 0.16656 13400.00 13600.00 20.00 0.16667 13400.00 13600.00 25.00 0.16675 13400.00 13600.00 30.00 0.16680 13400.00 13600.00 35.00 0.16680 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13390.59 13529.82 30.88 0.16713 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/370.ctf NX, NY, NZ: 512 512 1 MODE : real