CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/369.mrc Output diagnostic file name Micrographs_0601/369.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/369.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2500.000 7602.000 Mean, RMS : 4488.814 213.8534 TITLE 1: emi2mrc: 369.emi Sun Jan 05 23:19:21 2014 TITLE 2: kV 300, Mag 78000x, Def 17230, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 45.681, Y 580.138, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03139 10200.00 10000.00 0.00 0.03305 10400.00 10000.00 0.00 0.03461 10600.00 10000.00 0.00 0.03649 10800.00 10000.00 0.00 0.03825 11000.00 10000.00 0.00 0.03999 11200.00 10000.00 0.00 0.04191 11400.00 10000.00 0.00 0.04393 11600.00 10000.00 0.00 0.04613 11800.00 10000.00 0.00 0.04859 12000.00 10000.00 0.00 0.05126 12200.00 10000.00 0.00 0.05407 12400.00 10000.00 0.00 0.05708 12600.00 10000.00 0.00 0.05985 12800.00 10000.00 0.00 0.06253 13000.00 10000.00 0.00 0.06519 13200.00 10000.00 0.00 0.06785 13400.00 10000.00 0.00 0.07055 13600.00 10000.00 0.00 0.07347 13800.00 10000.00 0.00 0.07684 14000.00 10000.00 0.00 0.08002 14200.00 10000.00 0.00 0.08337 14400.00 10000.00 0.00 0.08672 14600.00 10000.00 0.00 0.08914 14800.00 10000.00 0.00 0.09014 15000.00 10000.00 0.00 0.09026 14600.00 10200.00 0.00 0.09115 14800.00 10200.00 0.00 0.09210 15000.00 10200.00 0.00 0.09216 14600.00 10400.00 0.00 0.09305 14800.00 10400.00 0.00 0.09397 15000.00 10400.00 0.00 0.09400 14600.00 10600.00 0.00 0.09508 14800.00 10600.00 0.00 0.09597 14600.00 10800.00 0.00 0.09716 14800.00 10800.00 0.00 0.09799 14600.00 11000.00 0.00 0.09954 14800.00 11000.00 0.00 0.10032 14600.00 11200.00 0.00 0.10199 14800.00 11200.00 0.00 0.10275 14600.00 11400.00 0.00 0.10486 14800.00 11400.00 0.00 0.10558 14600.00 11600.00 0.00 0.10772 14800.00 11600.00 0.00 0.10837 14600.00 11800.00 0.00 0.11051 14800.00 11800.00 0.00 0.11109 14400.00 12000.00 0.00 0.11138 14600.00 12000.00 0.00 0.11359 14800.00 12000.00 0.00 0.11411 14400.00 12200.00 0.00 0.11468 14600.00 12200.00 0.00 0.11686 14800.00 12200.00 0.00 0.11730 14400.00 12400.00 0.00 0.11805 14600.00 12400.00 0.00 0.12020 14800.00 12400.00 0.00 0.12057 14400.00 12600.00 0.00 0.12126 14600.00 12600.00 0.00 0.12344 14800.00 12600.00 0.00 0.12374 14400.00 12800.00 0.00 0.12475 14600.00 12800.00 0.00 0.12694 14800.00 12800.00 0.00 0.12715 14400.00 13000.00 0.00 0.12818 14600.00 13000.00 0.00 0.13035 14800.00 13000.00 0.00 0.13039 14400.00 13200.00 0.00 0.13186 14600.00 13200.00 0.00 0.13396 14400.00 13400.00 0.00 0.13563 14600.00 13400.00 0.00 0.13762 14400.00 13600.00 0.00 0.13958 14600.00 13600.00 0.00 0.14139 14400.00 13800.00 0.00 0.14354 14600.00 13800.00 0.00 0.14511 14400.00 14000.00 0.00 0.14706 14600.00 14000.00 0.00 0.14834 14400.00 14200.00 0.00 0.15107 14600.00 14200.00 0.00 0.15174 14400.00 14400.00 0.00 0.15420 14400.00 14600.00 0.00 0.15521 14400.00 14600.00 5.00 0.15523 14400.00 14600.00 10.00 0.15524 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14429.21 14554.03 10.62 0.15536 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/369.ctf NX, NY, NZ: 512 512 1 MODE : real