CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/365.mrc Output diagnostic file name Micrographs_0601/365.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/365.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2318.000 7830.000 Mean, RMS : 4495.449 221.8148 TITLE 1: emi2mrc: 365.emi Sun Jan 05 23:16:01 2014 TITLE 2: kV 300, Mag 78000x, Def 17230, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 40.463, Y 583.692, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.00261 10200.00 10000.00 0.00 0.00285 10400.00 10000.00 0.00 0.00309 10600.00 10000.00 0.00 0.00351 10800.00 10000.00 0.00 0.00382 11000.00 10000.00 0.00 0.00395 11200.00 10000.00 0.00 0.00407 11400.00 10000.00 0.00 0.00426 11600.00 10000.00 0.00 0.00442 11800.00 10000.00 0.00 0.00475 12000.00 10000.00 0.00 0.00528 12200.00 10000.00 0.00 0.00578 12400.00 10000.00 0.00 0.00626 12600.00 10000.00 0.00 0.00688 12800.00 10000.00 0.00 0.00769 13000.00 10000.00 0.00 0.00847 13200.00 10000.00 0.00 0.00919 13400.00 10000.00 0.00 0.00991 13600.00 10000.00 0.00 0.01073 13800.00 10000.00 0.00 0.01160 14000.00 10000.00 0.00 0.01226 14200.00 10000.00 0.00 0.01300 14400.00 10000.00 0.00 0.01389 14600.00 10000.00 0.00 0.01485 14800.00 10000.00 0.00 0.01588 15000.00 10000.00 0.00 0.01697 15200.00 10000.00 0.00 0.01818 15400.00 10000.00 0.00 0.01925 15600.00 10000.00 0.00 0.02035 15800.00 10000.00 0.00 0.02147 16000.00 10000.00 0.00 0.02268 16200.00 10000.00 0.00 0.02411 16400.00 10000.00 0.00 0.02543 16600.00 10000.00 0.00 0.02672 16800.00 10000.00 0.00 0.02805 17000.00 10000.00 0.00 0.02944 17200.00 10000.00 0.00 0.03089 17400.00 10000.00 0.00 0.03231 17600.00 10000.00 0.00 0.03376 17800.00 10000.00 0.00 0.03520 18000.00 10000.00 0.00 0.03666 18200.00 10000.00 0.00 0.03817 18400.00 10000.00 0.00 0.03971 18600.00 10000.00 0.00 0.04136 18800.00 10000.00 0.00 0.04310 19000.00 10000.00 0.00 0.04484 19200.00 10000.00 0.00 0.04669 19400.00 10000.00 0.00 0.04861 19600.00 10000.00 0.00 0.05056 19800.00 10000.00 0.00 0.05270 20000.00 10000.00 0.00 0.05479 20200.00 10000.00 0.00 0.05638 20400.00 10000.00 0.00 0.05729 20600.00 10000.00 0.00 0.05792 20800.00 10000.00 0.00 0.05834 21000.00 10000.00 0.00 0.05851 21200.00 10000.00 0.00 0.05858 20600.00 10200.00 0.00 0.05884 20800.00 10200.00 0.00 0.05932 21000.00 10200.00 0.00 0.05949 21200.00 10200.00 0.00 0.05953 20600.00 10400.00 0.00 0.05990 20800.00 10400.00 0.00 0.06035 21000.00 10400.00 0.00 0.06047 20600.00 10600.00 0.00 0.06084 20800.00 10600.00 0.00 0.06123 21000.00 10600.00 0.00 0.06131 20600.00 10800.00 0.00 0.06160 20800.00 10800.00 0.00 0.06198 21000.00 10800.00 0.00 0.06207 20600.00 11000.00 0.00 0.06240 20800.00 11000.00 0.00 0.06279 21000.00 11000.00 0.00 0.06289 20600.00 11200.00 0.00 0.06319 20800.00 11200.00 0.00 0.06359 21000.00 11200.00 0.00 0.06370 20600.00 11400.00 0.00 0.06412 20800.00 11400.00 0.00 0.06454 21000.00 11400.00 0.00 0.06465 20600.00 11600.00 0.00 0.06505 20800.00 11600.00 0.00 0.06550 21000.00 11600.00 0.00 0.06556 20600.00 11800.00 0.00 0.06604 20800.00 11800.00 0.00 0.06646 21000.00 11800.00 0.00 0.06646 20600.00 12000.00 0.00 0.06703 20800.00 12000.00 0.00 0.06743 20600.00 12200.00 0.00 0.06804 20800.00 12200.00 0.00 0.06840 20400.00 12400.00 0.00 0.06846 20600.00 12400.00 0.00 0.06916 20800.00 12400.00 0.00 0.06949 20400.00 12600.00 0.00 0.06964 20600.00 12600.00 0.00 0.07033 20800.00 12600.00 0.00 0.07060 20400.00 12800.00 0.00 0.07090 20600.00 12800.00 0.00 0.07152 20800.00 12800.00 0.00 0.07175 20400.00 13000.00 0.00 0.07199 20600.00 13000.00 0.00 0.07257 20800.00 13000.00 0.00 0.07279 20400.00 13200.00 0.00 0.07315 20600.00 13200.00 0.00 0.07374 20800.00 13200.00 0.00 0.07398 20400.00 13400.00 0.00 0.07444 20600.00 13400.00 0.00 0.07504 20800.00 13400.00 0.00 0.07527 20400.00 13600.00 0.00 0.07582 20600.00 13600.00 0.00 0.07640 20800.00 13600.00 0.00 0.07656 20400.00 13800.00 0.00 0.07719 20600.00 13800.00 0.00 0.07772 20800.00 13800.00 0.00 0.07783 20400.00 14000.00 0.00 0.07847 20600.00 14000.00 0.00 0.07897 20800.00 14000.00 0.00 0.07908 20400.00 14200.00 0.00 0.08003 20600.00 14200.00 0.00 0.08056 20800.00 14200.00 0.00 0.08068 20200.00 14400.00 0.00 0.08073 20400.00 14400.00 0.00 0.08176 20600.00 14400.00 0.00 0.08228 20800.00 14400.00 0.00 0.08234 20200.00 14600.00 0.00 0.08250 20400.00 14600.00 0.00 0.08349 20600.00 14600.00 0.00 0.08394 20200.00 14800.00 0.00 0.08416 20400.00 14800.00 0.00 0.08509 20600.00 14800.00 0.00 0.08549 20200.00 15000.00 0.00 0.08582 20400.00 15000.00 0.00 0.08671 20600.00 15000.00 0.00 0.08707 20200.00 15200.00 0.00 0.08749 20400.00 15200.00 0.00 0.08839 20600.00 15200.00 0.00 0.08875 20200.00 15400.00 0.00 0.08932 20400.00 15400.00 0.00 0.09024 20600.00 15400.00 0.00 0.09055 20200.00 15600.00 0.00 0.09129 20400.00 15600.00 0.00 0.09216 20600.00 15600.00 0.00 0.09241 20200.00 15800.00 0.00 0.09308 20400.00 15800.00 0.00 0.09392 20600.00 15800.00 0.00 0.09412 20200.00 16000.00 0.00 0.09503 20400.00 16000.00 0.00 0.09586 20600.00 16000.00 0.00 0.09606 20200.00 16200.00 0.00 0.09711 20400.00 16200.00 0.00 0.09793 20600.00 16200.00 0.00 0.09810 20200.00 16400.00 0.00 0.09931 20400.00 16400.00 0.00 0.10008 20600.00 16400.00 0.00 0.10022 20200.00 16600.00 0.00 0.10137 20400.00 16600.00 0.00 0.10210 20600.00 16600.00 0.00 0.10219 20200.00 16800.00 0.00 0.10347 20400.00 16800.00 0.00 0.10414 20600.00 16800.00 0.00 0.10417 20200.00 17000.00 0.00 0.10561 20400.00 17000.00 0.00 0.10623 20200.00 17200.00 0.00 0.10770 20400.00 17200.00 0.00 0.10824 20000.00 17400.00 0.00 0.10824 20200.00 17400.00 0.00 0.10994 20400.00 17400.00 0.00 0.11043 20000.00 17600.00 0.00 0.11049 20200.00 17600.00 0.00 0.11220 20400.00 17600.00 0.00 0.11264 20000.00 17800.00 0.00 0.11296 20200.00 17800.00 0.00 0.11467 20400.00 17800.00 0.00 0.11504 20000.00 18000.00 0.00 0.11532 20200.00 18000.00 0.00 0.11701 20400.00 18000.00 0.00 0.11726 20000.00 18200.00 0.00 0.11784 20200.00 18200.00 0.00 0.11946 20400.00 18200.00 0.00 0.11960 20000.00 18400.00 0.00 0.12040 20200.00 18400.00 0.00 0.12195 20400.00 18400.00 0.00 0.12196 20000.00 18600.00 0.00 0.12294 20200.00 18600.00 0.00 0.12441 20000.00 18800.00 0.00 0.12574 20200.00 18800.00 0.00 0.12713 20000.00 19000.00 0.00 0.12855 20200.00 19000.00 0.00 0.12983 20000.00 19200.00 0.00 0.13183 20200.00 19200.00 0.00 0.13291 20000.00 19400.00 0.00 0.13488 20200.00 19400.00 0.00 0.13570 19800.00 19600.00 0.00 0.13607 20000.00 19600.00 0.00 0.13801 20200.00 19600.00 0.00 0.13852 19800.00 19800.00 0.00 0.13946 20000.00 19800.00 0.00 0.14097 20200.00 19800.00 0.00 0.14104 19800.00 20000.00 0.00 0.14156 20000.00 20000.00 0.00 0.14276 20000.00 20200.00 15.00 0.14280 20000.00 20200.00 20.00 0.14293 19800.00 20200.00 25.00 0.14295 20000.00 20200.00 25.00 0.14303 19800.00 20200.00 30.00 0.14307 20000.00 20200.00 30.00 0.14312 19800.00 20200.00 35.00 0.14314 20000.00 20200.00 35.00 0.14318 20000.00 20200.00 40.00 0.14321 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 19931.67 20152.33 40.00 0.14349 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/365.ctf NX, NY, NZ: 512 512 1 MODE : real