CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/364.mrc Output diagnostic file name Micrographs_0601/364.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/364.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 1776.000 7960.000 Mean, RMS : 4520.700 222.3154 TITLE 1: emi2mrc: 364.emi Sun Jan 05 23:15:08 2014 TITLE 2: kV 300, Mag 78000x, Def 17390, Spot 4, Int 55.07%, Obj 88.07%, Dif 73.67% TITLE 3: X 42.978, Y 583.623, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01169 14600.00 10000.00 0.00 -0.01169 14800.00 10000.00 0.00 -0.01151 15000.00 10000.00 0.00 -0.01128 15200.00 10000.00 0.00 -0.01095 15400.00 10000.00 0.00 -0.01047 15600.00 10000.00 0.00 -0.00994 15800.00 10000.00 0.00 -0.00963 16000.00 10000.00 0.00 -0.00922 16200.00 10000.00 0.00 -0.00873 16400.00 10000.00 0.00 -0.00830 16600.00 10000.00 0.00 -0.00772 16800.00 10000.00 0.00 -0.00712 17000.00 10000.00 0.00 -0.00658 17200.00 10000.00 0.00 -0.00598 17400.00 10000.00 0.00 -0.00532 17600.00 10000.00 0.00 -0.00479 17800.00 10000.00 0.00 -0.00424 18000.00 10000.00 0.00 -0.00351 18200.00 10000.00 0.00 -0.00289 18400.00 10000.00 0.00 -0.00227 18600.00 10000.00 0.00 -0.00153 18800.00 10000.00 0.00 -0.00078 19000.00 10000.00 0.00 0.00003 19200.00 10000.00 0.00 0.00095 19400.00 10000.00 0.00 0.00196 19600.00 10000.00 0.00 0.00295 19800.00 10000.00 0.00 0.00387 20000.00 10000.00 0.00 0.00465 20200.00 10000.00 0.00 0.00552 20400.00 10000.00 0.00 0.00663 20600.00 10000.00 0.00 0.00767 20800.00 10000.00 0.00 0.00864 21000.00 10000.00 0.00 0.00958 21200.00 10000.00 0.00 0.01045 21400.00 10000.00 0.00 0.01135 21600.00 10000.00 0.00 0.01224 21800.00 10000.00 0.00 0.01315 22000.00 10000.00 0.00 0.01427 22200.00 10000.00 0.00 0.01541 22400.00 10000.00 0.00 0.01655 22600.00 10000.00 0.00 0.01782 22800.00 10000.00 0.00 0.01918 23000.00 10000.00 0.00 0.02055 23200.00 10000.00 0.00 0.02190 23400.00 10000.00 0.00 0.02336 23600.00 10000.00 0.00 0.02479 23800.00 10000.00 0.00 0.02618 24000.00 10000.00 0.00 0.02772 24200.00 10000.00 0.00 0.02939 24400.00 10000.00 0.00 0.03117 24600.00 10000.00 0.00 0.03301 24800.00 10000.00 0.00 0.03478 25000.00 10000.00 0.00 0.03607 25200.00 10000.00 0.00 0.03681 25400.00 10000.00 0.00 0.03721 25600.00 10000.00 0.00 0.03738 25800.00 10000.00 0.00 0.03742 25600.00 10200.00 0.00 0.03743 25800.00 10200.00 0.00 0.03744 25400.00 10400.00 0.00 0.03744 25600.00 10400.00 0.00 0.03756 25800.00 10400.00 0.00 0.03757 25600.00 10600.00 0.00 0.03769 25800.00 10600.00 0.00 0.03769 25600.00 10800.00 0.00 0.03773 25600.00 11000.00 0.00 0.03777 25800.00 11000.00 0.00 0.03779 25400.00 11200.00 0.00 0.03786 25600.00 11200.00 0.00 0.03800 25800.00 11200.00 0.00 0.03804 25400.00 11400.00 0.00 0.03822 25600.00 11400.00 0.00 0.03833 25800.00 11400.00 0.00 0.03838 25400.00 11600.00 0.00 0.03846 25600.00 11600.00 0.00 0.03858 25800.00 11600.00 0.00 0.03866 25400.00 11800.00 0.00 0.03877 25600.00 11800.00 0.00 0.03895 25800.00 11800.00 0.00 0.03905 25400.00 12000.00 0.00 0.03909 25600.00 12000.00 0.00 0.03933 25800.00 12000.00 0.00 0.03940 25400.00 12200.00 0.00 0.03945 25600.00 12200.00 0.00 0.03970 25800.00 12200.00 0.00 0.03973 25400.00 12400.00 0.00 0.03988 25600.00 12400.00 0.00 0.04012 25800.00 12400.00 0.00 0.04014 25400.00 12600.00 0.00 0.04025 25600.00 12600.00 0.00 0.04051 25800.00 12600.00 0.00 0.04056 25400.00 12800.00 0.00 0.04085 25600.00 12800.00 0.00 0.04113 25800.00 12800.00 0.00 0.04119 25400.00 13000.00 0.00 0.04147 25600.00 13000.00 0.00 0.04174 25800.00 13000.00 0.00 0.04178 25400.00 13200.00 0.00 0.04199 25600.00 13200.00 0.00 0.04225 25800.00 13200.00 0.00 0.04229 25400.00 13400.00 0.00 0.04256 25600.00 13400.00 0.00 0.04285 25800.00 13400.00 0.00 0.04291 25400.00 13600.00 0.00 0.04325 25600.00 13600.00 0.00 0.04356 25800.00 13600.00 0.00 0.04363 25400.00 13800.00 0.00 0.04395 25600.00 13800.00 0.00 0.04422 25800.00 13800.00 0.00 0.04425 25400.00 14000.00 0.00 0.04450 25600.00 14000.00 0.00 0.04475 25800.00 14000.00 0.00 0.04479 25400.00 14200.00 0.00 0.04514 25600.00 14200.00 0.00 0.04543 25800.00 14200.00 0.00 0.04551 25400.00 14400.00 0.00 0.04607 25600.00 14400.00 0.00 0.04637 25800.00 14400.00 0.00 0.04645 25200.00 14600.00 0.00 0.04659 25400.00 14600.00 0.00 0.04720 25600.00 14600.00 0.00 0.04744 25800.00 14600.00 0.00 0.04746 25200.00 14800.00 0.00 0.04761 25400.00 14800.00 0.00 0.04813 25600.00 14800.00 0.00 0.04830 25200.00 15000.00 0.00 0.04844 25400.00 15000.00 0.00 0.04890 25600.00 15000.00 0.00 0.04905 25200.00 15200.00 0.00 0.04927 25400.00 15200.00 0.00 0.04971 25600.00 15200.00 0.00 0.04988 25200.00 15400.00 0.00 0.05006 25400.00 15400.00 0.00 0.05051 25600.00 15400.00 0.00 0.05068 25200.00 15600.00 0.00 0.05087 25400.00 15600.00 0.00 0.05135 25600.00 15600.00 0.00 0.05152 25200.00 15800.00 0.00 0.05172 25400.00 15800.00 0.00 0.05224 25600.00 15800.00 0.00 0.05241 25200.00 16000.00 0.00 0.05268 25400.00 16000.00 0.00 0.05321 25600.00 16000.00 0.00 0.05335 25200.00 16200.00 0.00 0.05371 25400.00 16200.00 0.00 0.05423 25600.00 16200.00 0.00 0.05431 25200.00 16400.00 0.00 0.05475 25400.00 16400.00 0.00 0.05522 25600.00 16400.00 0.00 0.05523 25200.00 16600.00 0.00 0.05581 25400.00 16600.00 0.00 0.05623 25200.00 16800.00 0.00 0.05693 25400.00 16800.00 0.00 0.05733 25600.00 16800.00 0.00 0.05736 25200.00 17000.00 0.00 0.05798 25400.00 17000.00 0.00 0.05839 25600.00 17000.00 0.00 0.05843 25200.00 17200.00 0.00 0.05909 25400.00 17200.00 0.00 0.05951 25200.00 17400.00 0.00 0.06037 25400.00 17400.00 0.00 0.06073 25200.00 17600.00 0.00 0.06141 25400.00 17600.00 0.00 0.06170 25200.00 17800.00 0.00 0.06243 25400.00 17800.00 0.00 0.06268 25000.00 18000.00 0.00 0.06280 25200.00 18000.00 0.00 0.06365 25400.00 18000.00 0.00 0.06386 25000.00 18200.00 0.00 0.06407 25200.00 18200.00 0.00 0.06488 25400.00 18200.00 0.00 0.06504 25000.00 18400.00 0.00 0.06529 25200.00 18400.00 0.00 0.06605 25400.00 18400.00 0.00 0.06618 25000.00 18600.00 0.00 0.06650 25200.00 18600.00 0.00 0.06723 25400.00 18600.00 0.00 0.06732 25000.00 18800.00 0.00 0.06767 25200.00 18800.00 0.00 0.06837 25400.00 18800.00 0.00 0.06844 25000.00 19000.00 0.00 0.06881 25200.00 19000.00 0.00 0.06947 25400.00 19000.00 0.00 0.06952 25000.00 19200.00 0.00 0.07003 25200.00 19200.00 0.00 0.07065 25400.00 19200.00 0.00 0.07072 25000.00 19400.00 0.00 0.07134 25200.00 19400.00 0.00 0.07197 25400.00 19400.00 0.00 0.07207 25000.00 19600.00 0.00 0.07276 25200.00 19600.00 0.00 0.07343 25400.00 19600.00 0.00 0.07357 25000.00 19800.00 0.00 0.07418 25200.00 19800.00 0.00 0.07490 25400.00 19800.00 0.00 0.07503 25000.00 20000.00 0.00 0.07577 25200.00 20000.00 0.00 0.07652 25400.00 20000.00 0.00 0.07659 25000.00 20200.00 0.00 0.07747 25200.00 20200.00 0.00 0.07818 25000.00 20400.00 0.00 0.07900 25200.00 20400.00 0.00 0.07965 25000.00 20600.00 0.00 0.08051 25200.00 20600.00 0.00 0.08109 25000.00 20800.00 0.00 0.08196 25200.00 20800.00 0.00 0.08248 25000.00 21000.00 0.00 0.08342 25200.00 21000.00 0.00 0.08390 25000.00 21200.00 0.00 0.08492 25200.00 21200.00 0.00 0.08535 25000.00 21400.00 0.00 0.08648 25200.00 21400.00 0.00 0.08686 25000.00 21600.00 0.00 0.08807 25200.00 21600.00 0.00 0.08839 24800.00 21800.00 0.00 0.08854 25000.00 21800.00 0.00 0.08983 25200.00 21800.00 0.00 0.09010 24800.00 22000.00 0.00 0.09034 25000.00 22000.00 0.00 0.09158 25200.00 22000.00 0.00 0.09179 24800.00 22200.00 0.00 0.09204 25000.00 22200.00 0.00 0.09325 25200.00 22200.00 0.00 0.09342 24800.00 22400.00 0.00 0.09396 25000.00 22400.00 0.00 0.09512 25200.00 22400.00 0.00 0.09522 24800.00 22600.00 0.00 0.09580 25000.00 22600.00 0.00 0.09692 25200.00 22600.00 0.00 0.09701 24800.00 22800.00 0.00 0.09791 25000.00 22800.00 0.00 0.09903 25200.00 22800.00 0.00 0.09913 24800.00 23000.00 0.00 0.10009 25000.00 23000.00 0.00 0.10119 25200.00 23000.00 0.00 0.10125 24800.00 23200.00 0.00 0.10223 25000.00 23200.00 0.00 0.10333 24800.00 23400.00 0.00 0.10466 25000.00 23400.00 0.00 0.10570 24800.00 23600.00 0.00 0.10714 25000.00 23600.00 0.00 0.10807 24800.00 23800.00 0.00 0.10971 25000.00 23800.00 0.00 0.11046 24800.00 24000.00 0.00 0.11204 25000.00 24000.00 0.00 0.11258 24800.00 24200.00 0.00 0.11454 25000.00 24200.00 0.00 0.11479 24600.00 24400.00 0.00 0.11481 24800.00 24400.00 0.00 0.11673 24600.00 24600.00 0.00 0.11687 24800.00 24600.00 0.00 0.11852 24800.00 24800.00 0.00 0.11948 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24880.45 24727.12 -47.05 0.11976 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/364.ctf NX, NY, NZ: 512 512 1 MODE : real