CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/362.mrc Output diagnostic file name Micrographs_0601/362.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/362.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2480.000 8348.000 Mean, RMS : 4493.897 219.3496 TITLE 1: emi2mrc: 362.emi Sun Jan 05 23:13:38 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 48.255, Y 583.021, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.01850 10200.00 10000.00 0.00 0.01912 10400.00 10000.00 0.00 0.01977 10600.00 10000.00 0.00 0.02071 10800.00 10000.00 0.00 0.02161 11000.00 10000.00 0.00 0.02246 11200.00 10000.00 0.00 0.02332 11400.00 10000.00 0.00 0.02435 11600.00 10000.00 0.00 0.02547 11800.00 10000.00 0.00 0.02662 12000.00 10000.00 0.00 0.02789 12200.00 10000.00 0.00 0.02911 12400.00 10000.00 0.00 0.03049 12600.00 10000.00 0.00 0.03195 12800.00 10000.00 0.00 0.03354 13000.00 10000.00 0.00 0.03521 13200.00 10000.00 0.00 0.03684 13400.00 10000.00 0.00 0.03856 13600.00 10000.00 0.00 0.04037 13800.00 10000.00 0.00 0.04238 14000.00 10000.00 0.00 0.04463 14200.00 10000.00 0.00 0.04702 14400.00 10000.00 0.00 0.04935 14600.00 10000.00 0.00 0.05179 14800.00 10000.00 0.00 0.05421 15000.00 10000.00 0.00 0.05656 15200.00 10000.00 0.00 0.05901 15400.00 10000.00 0.00 0.06147 15600.00 10000.00 0.00 0.06427 15800.00 10000.00 0.00 0.06708 16000.00 10000.00 0.00 0.06996 16200.00 10000.00 0.00 0.07307 16400.00 10000.00 0.00 0.07627 16600.00 10000.00 0.00 0.07899 16800.00 10000.00 0.00 0.08027 17000.00 10000.00 0.00 0.08068 16800.00 10200.00 0.00 0.08153 17000.00 10200.00 0.00 0.08191 16800.00 10400.00 0.00 0.08281 17000.00 10400.00 0.00 0.08315 16800.00 10600.00 0.00 0.08416 17000.00 10600.00 0.00 0.08449 16800.00 10800.00 0.00 0.08551 17000.00 10800.00 0.00 0.08580 16600.00 11000.00 0.00 0.08589 16800.00 11000.00 0.00 0.08718 17000.00 11000.00 0.00 0.08742 16600.00 11200.00 0.00 0.08755 16800.00 11200.00 0.00 0.08882 17000.00 11200.00 0.00 0.08904 16600.00 11400.00 0.00 0.08941 16800.00 11400.00 0.00 0.09067 17000.00 11400.00 0.00 0.09092 16600.00 11600.00 0.00 0.09131 16800.00 11600.00 0.00 0.09256 17000.00 11600.00 0.00 0.09279 16600.00 11800.00 0.00 0.09317 16800.00 11800.00 0.00 0.09437 17000.00 11800.00 0.00 0.09456 16600.00 12000.00 0.00 0.09509 16800.00 12000.00 0.00 0.09629 17000.00 12000.00 0.00 0.09648 16600.00 12200.00 0.00 0.09719 16800.00 12200.00 0.00 0.09839 17000.00 12200.00 0.00 0.09859 16600.00 12400.00 0.00 0.09946 16800.00 12400.00 0.00 0.10066 17000.00 12400.00 0.00 0.10082 16600.00 12600.00 0.00 0.10169 16800.00 12600.00 0.00 0.10287 17000.00 12600.00 0.00 0.10300 16600.00 12800.00 0.00 0.10415 16800.00 12800.00 0.00 0.10534 17000.00 12800.00 0.00 0.10543 16600.00 13000.00 0.00 0.10653 16800.00 13000.00 0.00 0.10771 16600.00 13200.00 0.00 0.10900 16800.00 13200.00 0.00 0.11013 16600.00 13400.00 0.00 0.11148 16800.00 13400.00 0.00 0.11256 16600.00 13600.00 0.00 0.11399 16800.00 13600.00 0.00 0.11504 16600.00 13800.00 0.00 0.11663 16800.00 13800.00 0.00 0.11768 16600.00 14000.00 0.00 0.11935 16800.00 14000.00 0.00 0.12040 16600.00 14200.00 0.00 0.12236 16800.00 14200.00 0.00 0.12337 16600.00 14400.00 0.00 0.12540 16800.00 14400.00 0.00 0.12634 16600.00 14600.00 0.00 0.12873 16800.00 14600.00 0.00 0.12950 16600.00 14800.00 0.00 0.13179 16800.00 14800.00 0.00 0.13239 16600.00 15000.00 0.00 0.13498 16800.00 15000.00 0.00 0.13540 16600.00 15200.00 0.00 0.13833 16800.00 15200.00 0.00 0.13850 16600.00 15400.00 0.00 0.14157 16400.00 15600.00 0.00 0.14192 16600.00 15600.00 0.00 0.14491 16400.00 15800.00 0.00 0.14522 16600.00 15800.00 0.00 0.14809 16400.00 16000.00 0.00 0.14880 16600.00 16000.00 0.00 0.15141 16400.00 16200.00 0.00 0.15171 16600.00 16200.00 0.00 0.15402 16400.00 16400.00 0.00 0.15497 16600.00 16400.00 0.00 0.15655 16400.00 16600.00 0.00 0.15689 16600.00 16600.00 0.00 0.15702 16400.00 16600.00 5.00 0.15703 16400.00 16600.00 10.00 0.15717 16400.00 16600.00 15.00 0.15730 16400.00 16600.00 20.00 0.15741 16400.00 16600.00 25.00 0.15750 16400.00 16600.00 30.00 0.15757 16400.00 16600.00 35.00 0.15761 16400.00 16600.00 40.00 0.15763 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 16484.66 16600.00 35.95 0.15807 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/362.ctf NX, NY, NZ: 512 512 1 MODE : real