CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/361.mrc Output diagnostic file name Micrographs_0601/361.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/361.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2460.000 8278.000 Mean, RMS : 4492.792 219.5222 TITLE 1: emi2mrc: 361.emi Sun Jan 05 23:13:00 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 50.854, Y 582.742, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02213 10200.00 10000.00 0.00 0.02317 10400.00 10000.00 0.00 0.02422 10600.00 10000.00 0.00 0.02549 10800.00 10000.00 0.00 0.02654 11000.00 10000.00 0.00 0.02772 11200.00 10000.00 0.00 0.02910 11400.00 10000.00 0.00 0.03053 11600.00 10000.00 0.00 0.03207 11800.00 10000.00 0.00 0.03365 12000.00 10000.00 0.00 0.03544 12200.00 10000.00 0.00 0.03733 12400.00 10000.00 0.00 0.03939 12600.00 10000.00 0.00 0.04153 12800.00 10000.00 0.00 0.04369 13000.00 10000.00 0.00 0.04591 13200.00 10000.00 0.00 0.04829 13400.00 10000.00 0.00 0.05079 13600.00 10000.00 0.00 0.05333 13800.00 10000.00 0.00 0.05595 14000.00 10000.00 0.00 0.05837 14200.00 10000.00 0.00 0.06094 14400.00 10000.00 0.00 0.06376 14600.00 10000.00 0.00 0.06668 14800.00 10000.00 0.00 0.06969 15000.00 10000.00 0.00 0.07278 15200.00 10000.00 0.00 0.07624 15400.00 10000.00 0.00 0.08000 15600.00 10000.00 0.00 0.08356 15800.00 10000.00 0.00 0.08579 16000.00 10000.00 0.00 0.08664 16200.00 10000.00 0.00 0.08672 15800.00 10200.00 0.00 0.08735 16000.00 10200.00 0.00 0.08818 16200.00 10200.00 0.00 0.08822 15800.00 10400.00 0.00 0.08906 16000.00 10400.00 0.00 0.08986 15800.00 10600.00 0.00 0.09084 16000.00 10600.00 0.00 0.09159 15800.00 10800.00 0.00 0.09258 16000.00 10800.00 0.00 0.09331 15800.00 11000.00 0.00 0.09477 16000.00 11000.00 0.00 0.09546 15800.00 11200.00 0.00 0.09685 16000.00 11200.00 0.00 0.09748 15800.00 11400.00 0.00 0.09900 16000.00 11400.00 0.00 0.09953 15800.00 11600.00 0.00 0.10108 16000.00 11600.00 0.00 0.10151 15800.00 11800.00 0.00 0.10328 16000.00 11800.00 0.00 0.10364 15800.00 12000.00 0.00 0.10573 16000.00 12000.00 0.00 0.10603 15600.00 12200.00 0.00 0.10614 15800.00 12200.00 0.00 0.10828 16000.00 12200.00 0.00 0.10853 15600.00 12400.00 0.00 0.10886 15800.00 12400.00 0.00 0.11102 16000.00 12400.00 0.00 0.11125 15600.00 12600.00 0.00 0.11149 15800.00 12600.00 0.00 0.11368 16000.00 12600.00 0.00 0.11384 15600.00 12800.00 0.00 0.11436 15800.00 12800.00 0.00 0.11654 16000.00 12800.00 0.00 0.11661 15600.00 13000.00 0.00 0.11717 15800.00 13000.00 0.00 0.11933 15600.00 13200.00 0.00 0.12018 15800.00 13200.00 0.00 0.12230 15600.00 13400.00 0.00 0.12334 15800.00 13400.00 0.00 0.12541 15600.00 13600.00 0.00 0.12663 15800.00 13600.00 0.00 0.12866 15600.00 13800.00 0.00 0.13001 15800.00 13800.00 0.00 0.13197 15600.00 14000.00 0.00 0.13322 15800.00 14000.00 0.00 0.13509 15600.00 14200.00 0.00 0.13664 15800.00 14200.00 0.00 0.13840 15600.00 14400.00 0.00 0.14002 15800.00 14400.00 0.00 0.14164 15600.00 14600.00 0.00 0.14396 15800.00 14600.00 0.00 0.14536 15600.00 14800.00 0.00 0.14792 15800.00 14800.00 0.00 0.14901 15600.00 15000.00 0.00 0.15207 15800.00 15000.00 0.00 0.15273 15600.00 15200.00 0.00 0.15599 15800.00 15200.00 0.00 0.15605 15600.00 15400.00 0.00 0.15957 15600.00 15600.00 0.00 0.16253 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15580.49 15676.50 34.00 0.16291 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/361.ctf NX, NY, NZ: 512 512 1 MODE : real