CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/360.mrc Output diagnostic file name Micrographs_0601/360.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/360.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2308.000 7834.000 Mean, RMS : 4493.094 214.1105 TITLE 1: emi2mrc: 360.emi Sun Jan 05 23:11:40 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 53.486, Y 582.478, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02635 10200.00 10000.00 0.00 0.02776 10400.00 10000.00 0.00 0.02913 10600.00 10000.00 0.00 0.03074 10800.00 10000.00 0.00 0.03220 11000.00 10000.00 0.00 0.03396 11200.00 10000.00 0.00 0.03604 11400.00 10000.00 0.00 0.03818 11600.00 10000.00 0.00 0.04033 11800.00 10000.00 0.00 0.04240 12000.00 10000.00 0.00 0.04465 12200.00 10000.00 0.00 0.04720 12400.00 10000.00 0.00 0.05006 12600.00 10000.00 0.00 0.05294 12800.00 10000.00 0.00 0.05585 13000.00 10000.00 0.00 0.05864 13200.00 10000.00 0.00 0.06154 13400.00 10000.00 0.00 0.06453 13600.00 10000.00 0.00 0.06742 13800.00 10000.00 0.00 0.07062 14000.00 10000.00 0.00 0.07386 14200.00 10000.00 0.00 0.07733 14400.00 10000.00 0.00 0.08093 14600.00 10000.00 0.00 0.08479 14800.00 10000.00 0.00 0.08872 15000.00 10000.00 0.00 0.09139 15200.00 10000.00 0.00 0.09243 15000.00 10200.00 0.00 0.09366 15200.00 10200.00 0.00 0.09463 15000.00 10400.00 0.00 0.09588 15200.00 10400.00 0.00 0.09675 15000.00 10600.00 0.00 0.09810 15200.00 10600.00 0.00 0.09885 15000.00 10800.00 0.00 0.10041 15200.00 10800.00 0.00 0.10105 15000.00 11000.00 0.00 0.10278 15200.00 11000.00 0.00 0.10329 15000.00 11200.00 0.00 0.10490 15200.00 11200.00 0.00 0.10536 15000.00 11400.00 0.00 0.10741 15200.00 11400.00 0.00 0.10783 15000.00 11600.00 0.00 0.10979 15200.00 11600.00 0.00 0.11015 15000.00 11800.00 0.00 0.11229 15200.00 11800.00 0.00 0.11258 14800.00 12000.00 0.00 0.11260 15000.00 12000.00 0.00 0.11489 15200.00 12000.00 0.00 0.11512 14800.00 12200.00 0.00 0.11523 15000.00 12200.00 0.00 0.11751 15200.00 12200.00 0.00 0.11763 14800.00 12400.00 0.00 0.11820 15000.00 12400.00 0.00 0.12040 14800.00 12600.00 0.00 0.12094 15000.00 12600.00 0.00 0.12302 14800.00 12800.00 0.00 0.12375 15000.00 12800.00 0.00 0.12573 14800.00 13000.00 0.00 0.12632 15000.00 13000.00 0.00 0.12827 14800.00 13200.00 0.00 0.12923 15000.00 13200.00 0.00 0.13110 14800.00 13400.00 0.00 0.13227 15000.00 13400.00 0.00 0.13393 14800.00 13600.00 0.00 0.13508 15000.00 13600.00 0.00 0.13648 14800.00 13800.00 0.00 0.13772 15000.00 13800.00 0.00 0.13887 14800.00 14000.00 0.00 0.14007 15000.00 14000.00 0.00 0.14100 14800.00 14200.00 0.00 0.14265 15000.00 14200.00 0.00 0.14326 14800.00 14400.00 0.00 0.14464 15000.00 14400.00 0.00 0.14487 14800.00 14600.00 0.00 0.14697 14800.00 14800.00 0.00 0.14855 14800.00 15000.00 0.00 0.14870 14800.00 15000.00 5.00 0.14876 14800.00 15000.00 10.00 0.14880 14800.00 15000.00 15.00 0.14883 14800.00 15000.00 20.00 0.14884 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14774.27 15000.00 22.07 0.14887 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/360.ctf NX, NY, NZ: 512 512 1 MODE : real