CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/359.mrc Output diagnostic file name Micrographs_0601/359.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/359.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2396.000 8046.000 Mean, RMS : 4488.027 220.1073 TITLE 1: emi2mrc: 359.emi Sun Jan 05 23:11:02 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 56.015, Y 581.954, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03382 10200.00 10000.00 0.00 0.03551 10400.00 10000.00 0.00 0.03712 10600.00 10000.00 0.00 0.03909 10800.00 10000.00 0.00 0.04118 11000.00 10000.00 0.00 0.04341 11200.00 10000.00 0.00 0.04563 11400.00 10000.00 0.00 0.04805 11600.00 10000.00 0.00 0.05071 11800.00 10000.00 0.00 0.05321 12000.00 10000.00 0.00 0.05609 12200.00 10000.00 0.00 0.05919 12400.00 10000.00 0.00 0.06229 12600.00 10000.00 0.00 0.06549 12800.00 10000.00 0.00 0.06878 13000.00 10000.00 0.00 0.07226 13200.00 10000.00 0.00 0.07596 13400.00 10000.00 0.00 0.07983 13600.00 10000.00 0.00 0.08384 13800.00 10000.00 0.00 0.08827 14000.00 10000.00 0.00 0.09242 14200.00 10000.00 0.00 0.09545 14400.00 10000.00 0.00 0.09682 14600.00 10000.00 0.00 0.09697 14200.00 10200.00 0.00 0.09807 14400.00 10200.00 0.00 0.09938 14600.00 10200.00 0.00 0.09942 14200.00 10400.00 0.00 0.10072 14400.00 10400.00 0.00 0.10195 14200.00 10600.00 0.00 0.10340 14400.00 10600.00 0.00 0.10455 14200.00 10800.00 0.00 0.10594 14400.00 10800.00 0.00 0.10703 14200.00 11000.00 0.00 0.10884 14400.00 11000.00 0.00 0.10991 14200.00 11200.00 0.00 0.11188 14400.00 11200.00 0.00 0.11288 14200.00 11400.00 0.00 0.11529 14400.00 11400.00 0.00 0.11619 14200.00 11600.00 0.00 0.11861 14400.00 11600.00 0.00 0.11946 14200.00 11800.00 0.00 0.12208 14400.00 11800.00 0.00 0.12287 14200.00 12000.00 0.00 0.12576 14400.00 12000.00 0.00 0.12646 14000.00 12200.00 0.00 0.12672 14200.00 12200.00 0.00 0.12961 14400.00 12200.00 0.00 0.13019 14000.00 12400.00 0.00 0.13092 14200.00 12400.00 0.00 0.13373 14400.00 12400.00 0.00 0.13413 14000.00 12600.00 0.00 0.13484 14200.00 12600.00 0.00 0.13754 14400.00 12600.00 0.00 0.13770 14000.00 12800.00 0.00 0.13901 14200.00 12800.00 0.00 0.14155 14000.00 13000.00 0.00 0.14288 14200.00 13000.00 0.00 0.14531 14000.00 13200.00 0.00 0.14724 14200.00 13200.00 0.00 0.14948 14000.00 13400.00 0.00 0.15162 14200.00 13400.00 0.00 0.15356 14000.00 13600.00 0.00 0.15593 14200.00 13600.00 0.00 0.15743 14000.00 13800.00 0.00 0.16039 14200.00 13800.00 0.00 0.16121 14000.00 14000.00 0.00 0.16317 14200.00 14000.00 0.00 0.16336 14000.00 14200.00 0.00 0.16431 14000.00 14200.00 5.00 0.16440 14000.00 14200.00 10.00 0.16448 14000.00 14200.00 15.00 0.16454 14000.00 14200.00 20.00 0.16459 14000.00 14200.00 25.00 0.16461 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14044.48 14169.75 24.45 0.16469 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/359.ctf NX, NY, NZ: 512 512 1 MODE : real