CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/358.mrc Output diagnostic file name Micrographs_0601/358.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/358.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2192.000 9012.000 Mean, RMS : 4494.201 216.8721 TITLE 1: emi2mrc: 358.emi Sun Jan 05 23:10:25 2014 TITLE 2: kV 300, Mag 78000x, Def 17880, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 58.566, Y 581.683, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04914 10200.00 10000.00 0.00 0.05140 10400.00 10000.00 0.00 0.05362 10600.00 10000.00 0.00 0.05619 10800.00 10000.00 0.00 0.05871 11000.00 10000.00 0.00 0.06172 11200.00 10000.00 0.00 0.06489 11400.00 10000.00 0.00 0.06817 11600.00 10000.00 0.00 0.07175 11800.00 10000.00 0.00 0.07524 12000.00 10000.00 0.00 0.07903 12200.00 10000.00 0.00 0.08283 12400.00 10000.00 0.00 0.08680 12600.00 10000.00 0.00 0.09085 12800.00 10000.00 0.00 0.09512 13000.00 10000.00 0.00 0.09956 13200.00 10000.00 0.00 0.10359 13400.00 10000.00 0.00 0.10676 13600.00 10000.00 0.00 0.10834 13800.00 10000.00 0.00 0.10856 13400.00 10200.00 0.00 0.10977 13600.00 10200.00 0.00 0.11130 13800.00 10200.00 0.00 0.11139 13400.00 10400.00 0.00 0.11298 13600.00 10400.00 0.00 0.11437 13400.00 10600.00 0.00 0.11621 13600.00 10600.00 0.00 0.11751 13400.00 10800.00 0.00 0.11940 13600.00 10800.00 0.00 0.12067 13400.00 11000.00 0.00 0.12309 13600.00 11000.00 0.00 0.12433 13400.00 11200.00 0.00 0.12665 13600.00 11200.00 0.00 0.12784 13400.00 11400.00 0.00 0.13058 13600.00 11400.00 0.00 0.13165 13400.00 11600.00 0.00 0.13441 13600.00 11600.00 0.00 0.13531 13400.00 11800.00 0.00 0.13842 13600.00 11800.00 0.00 0.13915 13200.00 12000.00 0.00 0.13956 13400.00 12000.00 0.00 0.14266 13600.00 12000.00 0.00 0.14319 13200.00 12200.00 0.00 0.14381 13400.00 12200.00 0.00 0.14686 13600.00 12200.00 0.00 0.14712 13200.00 12400.00 0.00 0.14841 13400.00 12400.00 0.00 0.15135 13200.00 12600.00 0.00 0.15280 13400.00 12600.00 0.00 0.15557 13200.00 12800.00 0.00 0.15759 13400.00 12800.00 0.00 0.16005 13200.00 13000.00 0.00 0.16176 13400.00 13000.00 0.00 0.16387 13200.00 13200.00 0.00 0.16590 13400.00 13200.00 0.00 0.16726 13200.00 13400.00 0.00 0.16811 13200.00 13400.00 5.00 0.16817 13200.00 13400.00 10.00 0.16821 13200.00 13400.00 15.00 0.16824 13200.00 13400.00 20.00 0.16824 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13305.42 13372.84 18.99 0.16877 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/358.ctf NX, NY, NZ: 512 512 1 MODE : real