CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/357.mrc Output diagnostic file name Micrographs_0601/357.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/357.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2380.000 8640.000 Mean, RMS : 4500.172 217.6138 TITLE 1: emi2mrc: 357.emi Sun Jan 05 23:08:35 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 63.810, Y 580.790, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02440 10200.00 10000.00 0.00 0.02554 10400.00 10000.00 0.00 0.02665 10600.00 10000.00 0.00 0.02800 10800.00 10000.00 0.00 0.02937 11000.00 10000.00 0.00 0.03092 11200.00 10000.00 0.00 0.03267 11400.00 10000.00 0.00 0.03440 11600.00 10000.00 0.00 0.03640 11800.00 10000.00 0.00 0.03861 12000.00 10000.00 0.00 0.04086 12200.00 10000.00 0.00 0.04312 12400.00 10000.00 0.00 0.04553 12600.00 10000.00 0.00 0.04795 12800.00 10000.00 0.00 0.05042 13000.00 10000.00 0.00 0.05316 13200.00 10000.00 0.00 0.05604 13400.00 10000.00 0.00 0.05883 13600.00 10000.00 0.00 0.06155 13800.00 10000.00 0.00 0.06427 14000.00 10000.00 0.00 0.06689 14200.00 10000.00 0.00 0.06991 14400.00 10000.00 0.00 0.07311 14600.00 10000.00 0.00 0.07640 14800.00 10000.00 0.00 0.07981 15000.00 10000.00 0.00 0.08279 15200.00 10000.00 0.00 0.08525 15400.00 10000.00 0.00 0.08671 15600.00 10000.00 0.00 0.08723 15400.00 10200.00 0.00 0.08833 15600.00 10200.00 0.00 0.08884 15400.00 10400.00 0.00 0.09018 15600.00 10400.00 0.00 0.09068 15200.00 10600.00 0.00 0.09069 15400.00 10600.00 0.00 0.09205 15600.00 10600.00 0.00 0.09257 15400.00 10800.00 0.00 0.09394 15600.00 10800.00 0.00 0.09453 15200.00 11000.00 0.00 0.09475 15400.00 11000.00 0.00 0.09620 15600.00 11000.00 0.00 0.09680 15200.00 11200.00 0.00 0.09701 15400.00 11200.00 0.00 0.09849 15600.00 11200.00 0.00 0.09903 15200.00 11400.00 0.00 0.09950 15400.00 11400.00 0.00 0.10099 15600.00 11400.00 0.00 0.10148 15200.00 11600.00 0.00 0.10180 15400.00 11600.00 0.00 0.10333 15600.00 11600.00 0.00 0.10377 15200.00 11800.00 0.00 0.10429 15400.00 11800.00 0.00 0.10585 15600.00 11800.00 0.00 0.10622 15200.00 12000.00 0.00 0.10694 15400.00 12000.00 0.00 0.10843 15600.00 12000.00 0.00 0.10864 15200.00 12200.00 0.00 0.10958 15400.00 12200.00 0.00 0.11094 15600.00 12200.00 0.00 0.11102 15200.00 12400.00 0.00 0.11239 15400.00 12400.00 0.00 0.11365 15600.00 12400.00 0.00 0.11367 15200.00 12600.00 0.00 0.11502 15400.00 12600.00 0.00 0.11627 15600.00 12600.00 0.00 0.11628 15200.00 12800.00 0.00 0.11809 15400.00 12800.00 0.00 0.11933 15200.00 13000.00 0.00 0.12107 15400.00 13000.00 0.00 0.12223 15200.00 13200.00 0.00 0.12424 15400.00 13200.00 0.00 0.12527 15200.00 13400.00 0.00 0.12749 15400.00 13400.00 0.00 0.12839 15000.00 13600.00 0.00 0.12840 15200.00 13600.00 0.00 0.13079 15400.00 13600.00 0.00 0.13156 15000.00 13800.00 0.00 0.13198 15200.00 13800.00 0.00 0.13430 15400.00 13800.00 0.00 0.13488 15000.00 14000.00 0.00 0.13535 15200.00 14000.00 0.00 0.13758 15400.00 14000.00 0.00 0.13793 15000.00 14200.00 0.00 0.13891 15200.00 14200.00 0.00 0.14100 15400.00 14200.00 0.00 0.14108 15000.00 14400.00 0.00 0.14203 15200.00 14400.00 0.00 0.14397 15000.00 14600.00 0.00 0.14526 15200.00 14600.00 0.00 0.14691 15000.00 14800.00 0.00 0.14777 15200.00 14800.00 0.00 0.14918 15000.00 15000.00 0.00 0.14985 15200.00 15000.00 0.00 0.15095 15000.00 15200.00 0.00 0.15126 15200.00 15200.00 0.00 0.15162 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15136.64 15183.81 4.36 0.15181 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/357.ctf NX, NY, NZ: 512 512 1 MODE : real