CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/352.mrc Output diagnostic file name Micrographs_0601/352.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/352.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2382.000 7590.000 Mean, RMS : 4490.417 214.5943 TITLE 1: emi2mrc: 352.emi Sun Jan 05 23:03:53 2014 TITLE 2: kV 300, Mag 78000x, Def 17720, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 62.079, Y 583.402, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04461 10200.00 10000.00 0.00 0.04695 10400.00 10000.00 0.00 0.04911 10600.00 10000.00 0.00 0.05143 10800.00 10000.00 0.00 0.05391 11000.00 10000.00 0.00 0.05681 11200.00 10000.00 0.00 0.05973 11400.00 10000.00 0.00 0.06270 11600.00 10000.00 0.00 0.06598 11800.00 10000.00 0.00 0.06930 12000.00 10000.00 0.00 0.07289 12200.00 10000.00 0.00 0.07648 12400.00 10000.00 0.00 0.08014 12600.00 10000.00 0.00 0.08380 12800.00 10000.00 0.00 0.08783 13000.00 10000.00 0.00 0.09215 13200.00 10000.00 0.00 0.09635 13400.00 10000.00 0.00 0.10058 13600.00 10000.00 0.00 0.10415 13800.00 10000.00 0.00 0.10624 14000.00 10000.00 0.00 0.10657 13600.00 10200.00 0.00 0.10660 13800.00 10200.00 0.00 0.10867 14000.00 10200.00 0.00 0.10898 13600.00 10400.00 0.00 0.10921 13800.00 10400.00 0.00 0.11130 14000.00 10400.00 0.00 0.11159 13600.00 10600.00 0.00 0.11206 13800.00 10600.00 0.00 0.11414 14000.00 10600.00 0.00 0.11438 13600.00 10800.00 0.00 0.11494 13800.00 10800.00 0.00 0.11698 14000.00 10800.00 0.00 0.11710 13600.00 11000.00 0.00 0.11815 13800.00 11000.00 0.00 0.12011 13600.00 11200.00 0.00 0.12113 13800.00 11200.00 0.00 0.12300 13600.00 11400.00 0.00 0.12433 13800.00 11400.00 0.00 0.12610 13600.00 11600.00 0.00 0.12738 13800.00 11600.00 0.00 0.12907 13600.00 11800.00 0.00 0.13042 13800.00 11800.00 0.00 0.13200 13600.00 12000.00 0.00 0.13366 13800.00 12000.00 0.00 0.13510 13600.00 12200.00 0.00 0.13692 13800.00 12200.00 0.00 0.13816 13600.00 12400.00 0.00 0.14018 13800.00 12400.00 0.00 0.14121 13600.00 12600.00 0.00 0.14311 13800.00 12600.00 0.00 0.14394 13600.00 12800.00 0.00 0.14622 13800.00 12800.00 0.00 0.14677 13600.00 13000.00 0.00 0.14866 13800.00 13000.00 0.00 0.14894 13400.00 13200.00 0.00 0.14904 13600.00 13200.00 0.00 0.15127 13400.00 13400.00 0.00 0.15188 13600.00 13400.00 0.00 0.15346 13600.00 13600.00 0.00 0.15469 13600.00 13800.00 0.00 0.15514 13600.00 13800.00 5.00 0.15519 13600.00 13800.00 10.00 0.15521 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13574.94 13763.13 17.86 0.15529 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/352.ctf NX, NY, NZ: 512 512 1 MODE : real