CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/342.mrc Output diagnostic file name Micrographs_0601/342.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/342.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2174.000 9038.000 Mean, RMS : 4488.550 217.4103 TITLE 1: emi2mrc: 342.emi Sun Jan 05 22:55:39 2014 TITLE 2: kV 300, Mag 78000x, Def 19030, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 38.509, Y 589.427, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01484 13800.00 10000.00 0.00 -0.01465 14000.00 10000.00 0.00 -0.01438 14200.00 10000.00 0.00 -0.01403 14400.00 10000.00 0.00 -0.01365 14600.00 10000.00 0.00 -0.01327 14800.00 10000.00 0.00 -0.01285 15000.00 10000.00 0.00 -0.01237 15200.00 10000.00 0.00 -0.01191 15400.00 10000.00 0.00 -0.01136 15600.00 10000.00 0.00 -0.01079 15800.00 10000.00 0.00 -0.01034 16000.00 10000.00 0.00 -0.01000 16200.00 10000.00 0.00 -0.00967 16400.00 10000.00 0.00 -0.00922 16600.00 10000.00 0.00 -0.00879 16800.00 10000.00 0.00 -0.00844 17000.00 10000.00 0.00 -0.00794 17200.00 10000.00 0.00 -0.00726 17400.00 10000.00 0.00 -0.00647 17600.00 10000.00 0.00 -0.00573 17800.00 10000.00 0.00 -0.00508 18000.00 10000.00 0.00 -0.00424 18200.00 10000.00 0.00 -0.00339 18400.00 10000.00 0.00 -0.00254 18600.00 10000.00 0.00 -0.00153 18800.00 10000.00 0.00 -0.00057 19000.00 10000.00 0.00 0.00033 19200.00 10000.00 0.00 0.00129 19400.00 10000.00 0.00 0.00233 19600.00 10000.00 0.00 0.00353 19800.00 10000.00 0.00 0.00480 20000.00 10000.00 0.00 0.00599 20200.00 10000.00 0.00 0.00704 20400.00 10000.00 0.00 0.00799 20600.00 10000.00 0.00 0.00903 20800.00 10000.00 0.00 0.01004 21000.00 10000.00 0.00 0.01109 21200.00 10000.00 0.00 0.01227 21400.00 10000.00 0.00 0.01335 21600.00 10000.00 0.00 0.01453 21800.00 10000.00 0.00 0.01582 22000.00 10000.00 0.00 0.01698 22200.00 10000.00 0.00 0.01821 22400.00 10000.00 0.00 0.01950 22600.00 10000.00 0.00 0.02071 22800.00 10000.00 0.00 0.02207 23000.00 10000.00 0.00 0.02366 23200.00 10000.00 0.00 0.02523 23400.00 10000.00 0.00 0.02676 23600.00 10000.00 0.00 0.02856 23800.00 10000.00 0.00 0.03058 24000.00 10000.00 0.00 0.03254 24200.00 10000.00 0.00 0.03463 24400.00 10000.00 0.00 0.03663 24600.00 10000.00 0.00 0.03782 24800.00 10000.00 0.00 0.03822 25000.00 10000.00 0.00 0.03833 25200.00 10000.00 0.00 0.03834 24800.00 10200.00 0.00 0.03847 25000.00 10200.00 0.00 0.03857 24800.00 10400.00 0.00 0.03884 25000.00 10400.00 0.00 0.03896 24800.00 10600.00 0.00 0.03942 25000.00 10600.00 0.00 0.03953 24600.00 10800.00 0.00 0.03958 24800.00 10800.00 0.00 0.04003 25000.00 10800.00 0.00 0.04009 24600.00 11000.00 0.00 0.04019 24800.00 11000.00 0.00 0.04057 25000.00 11000.00 0.00 0.04059 24600.00 11200.00 0.00 0.04069 24800.00 11200.00 0.00 0.04103 25000.00 11200.00 0.00 0.04105 24600.00 11400.00 0.00 0.04126 24800.00 11400.00 0.00 0.04159 25000.00 11400.00 0.00 0.04163 24600.00 11600.00 0.00 0.04186 24800.00 11600.00 0.00 0.04219 25000.00 11600.00 0.00 0.04226 24600.00 11800.00 0.00 0.04253 24800.00 11800.00 0.00 0.04287 25000.00 11800.00 0.00 0.04293 24600.00 12000.00 0.00 0.04317 24800.00 12000.00 0.00 0.04350 25000.00 12000.00 0.00 0.04355 24600.00 12200.00 0.00 0.04384 24800.00 12200.00 0.00 0.04419 25000.00 12200.00 0.00 0.04422 24600.00 12400.00 0.00 0.04465 24800.00 12400.00 0.00 0.04499 24600.00 12600.00 0.00 0.04530 24800.00 12600.00 0.00 0.04561 24600.00 12800.00 0.00 0.04590 24800.00 12800.00 0.00 0.04617 24600.00 13000.00 0.00 0.04637 24800.00 13000.00 0.00 0.04664 24600.00 13200.00 0.00 0.04694 24800.00 13200.00 0.00 0.04724 25000.00 13200.00 0.00 0.04726 24600.00 13400.00 0.00 0.04771 24800.00 13400.00 0.00 0.04802 25000.00 13400.00 0.00 0.04803 24600.00 13600.00 0.00 0.04840 24800.00 13600.00 0.00 0.04872 24600.00 13800.00 0.00 0.04912 24800.00 13800.00 0.00 0.04943 24600.00 14000.00 0.00 0.04994 24800.00 14000.00 0.00 0.05020 24600.00 14200.00 0.00 0.05077 24800.00 14200.00 0.00 0.05099 24600.00 14400.00 0.00 0.05152 24800.00 14400.00 0.00 0.05176 24600.00 14600.00 0.00 0.05235 24800.00 14600.00 0.00 0.05263 24600.00 14800.00 0.00 0.05321 24800.00 14800.00 0.00 0.05349 24600.00 15000.00 0.00 0.05398 24800.00 15000.00 0.00 0.05424 24600.00 15200.00 0.00 0.05472 24800.00 15200.00 0.00 0.05497 24600.00 15400.00 0.00 0.05548 24800.00 15400.00 0.00 0.05572 24600.00 15600.00 0.00 0.05630 24800.00 15600.00 0.00 0.05654 24600.00 15800.00 0.00 0.05724 24800.00 15800.00 0.00 0.05747 24600.00 16000.00 0.00 0.05834 24800.00 16000.00 0.00 0.05851 24600.00 16200.00 0.00 0.05929 24800.00 16200.00 0.00 0.05942 24600.00 16400.00 0.00 0.06027 24800.00 16400.00 0.00 0.06039 24600.00 16600.00 0.00 0.06126 24800.00 16600.00 0.00 0.06141 24600.00 16800.00 0.00 0.06220 24800.00 16800.00 0.00 0.06237 24600.00 17000.00 0.00 0.06326 24800.00 17000.00 0.00 0.06342 24600.00 17200.00 0.00 0.06429 24800.00 17200.00 0.00 0.06444 24600.00 17400.00 0.00 0.06528 24800.00 17400.00 0.00 0.06542 24400.00 17600.00 0.00 0.06546 24600.00 17600.00 0.00 0.06638 24800.00 17600.00 0.00 0.06654 24400.00 17800.00 0.00 0.06672 24600.00 17800.00 0.00 0.06765 24800.00 17800.00 0.00 0.06781 24400.00 18000.00 0.00 0.06804 24600.00 18000.00 0.00 0.06898 24800.00 18000.00 0.00 0.06914 24400.00 18200.00 0.00 0.06931 24600.00 18200.00 0.00 0.07028 24800.00 18200.00 0.00 0.07041 24400.00 18400.00 0.00 0.07060 24600.00 18400.00 0.00 0.07158 24800.00 18400.00 0.00 0.07167 24400.00 18600.00 0.00 0.07197 24600.00 18600.00 0.00 0.07291 24800.00 18600.00 0.00 0.07293 24400.00 18800.00 0.00 0.07312 24600.00 18800.00 0.00 0.07399 24400.00 19000.00 0.00 0.07414 24600.00 19000.00 0.00 0.07499 24400.00 19200.00 0.00 0.07534 24600.00 19200.00 0.00 0.07618 24400.00 19400.00 0.00 0.07647 24600.00 19400.00 0.00 0.07727 24400.00 19600.00 0.00 0.07768 24600.00 19600.00 0.00 0.07846 24400.00 19800.00 0.00 0.07885 24600.00 19800.00 0.00 0.07965 24400.00 20000.00 0.00 0.08005 24600.00 20000.00 0.00 0.08089 24400.00 20200.00 0.00 0.08137 24600.00 20200.00 0.00 0.08222 24400.00 20400.00 0.00 0.08262 24600.00 20400.00 0.00 0.08342 24400.00 20600.00 0.00 0.08395 24600.00 20600.00 0.00 0.08468 24400.00 20800.00 0.00 0.08526 24600.00 20800.00 0.00 0.08589 24400.00 21000.00 0.00 0.08665 24600.00 21000.00 0.00 0.08716 24400.00 21200.00 0.00 0.08807 24600.00 21200.00 0.00 0.08847 24400.00 21400.00 0.00 0.08952 24600.00 21400.00 0.00 0.08986 24400.00 21600.00 0.00 0.09086 24600.00 21600.00 0.00 0.09115 24400.00 21800.00 0.00 0.09207 24600.00 21800.00 0.00 0.09233 24400.00 22000.00 0.00 0.09335 24600.00 22000.00 0.00 0.09357 24400.00 22200.00 0.00 0.09459 24600.00 22200.00 0.00 0.09479 24400.00 22400.00 0.00 0.09601 24600.00 22400.00 0.00 0.09615 24400.00 22600.00 0.00 0.09732 24600.00 22600.00 0.00 0.09735 24400.00 22800.00 0.00 0.09854 24400.00 23000.00 0.00 0.09969 24400.00 23200.00 0.00 0.10083 24400.00 23400.00 0.00 0.10208 24400.00 23600.00 0.00 0.10337 24400.00 23800.00 0.00 0.10473 24400.00 24000.00 0.00 0.10560 24400.00 24200.00 0.00 0.10643 24400.00 24400.00 0.00 0.10656 24200.00 24400.00 25.00 0.10660 24200.00 24400.00 30.00 0.10668 24200.00 24400.00 35.00 0.10675 24200.00 24400.00 40.00 0.10681 24200.00 24400.00 45.00 0.10685 24200.00 24400.00 50.00 0.10687 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24200.00 24400.00 52.28 0.10687 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/342.ctf NX, NY, NZ: 512 512 1 MODE : real