CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/341.mrc Output diagnostic file name Micrographs_0601/341.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/341.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2520.000 9138.000 Mean, RMS : 4490.874 215.0379 TITLE 1: emi2mrc: 341.emi Sun Jan 05 22:54:56 2014 TITLE 2: kV 300, Mag 78000x, Def 19030, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 41.276, Y 589.217, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01040 10200.00 10000.00 0.00 -0.01031 10400.00 10000.00 0.00 -0.00988 10600.00 10000.00 0.00 -0.00963 10800.00 10000.00 0.00 -0.00957 11000.00 10000.00 0.00 -0.00939 11200.00 10000.00 0.00 -0.00911 11400.00 10000.00 0.00 -0.00883 11600.00 10000.00 0.00 -0.00866 11800.00 10000.00 0.00 -0.00843 12000.00 10000.00 0.00 -0.00824 12200.00 10000.00 0.00 -0.00814 12400.00 10000.00 0.00 -0.00793 12600.00 10000.00 0.00 -0.00772 12800.00 10000.00 0.00 -0.00743 13000.00 10000.00 0.00 -0.00715 13200.00 10000.00 0.00 -0.00692 13400.00 10000.00 0.00 -0.00639 13600.00 10000.00 0.00 -0.00596 13800.00 10000.00 0.00 -0.00571 14000.00 10000.00 0.00 -0.00530 14200.00 10000.00 0.00 -0.00486 14400.00 10000.00 0.00 -0.00441 14600.00 10000.00 0.00 -0.00380 14800.00 10000.00 0.00 -0.00317 15000.00 10000.00 0.00 -0.00259 15200.00 10000.00 0.00 -0.00203 15400.00 10000.00 0.00 -0.00143 15600.00 10000.00 0.00 -0.00067 15800.00 10000.00 0.00 0.00012 16000.00 10000.00 0.00 0.00081 16200.00 10000.00 0.00 0.00155 16400.00 10000.00 0.00 0.00242 16600.00 10000.00 0.00 0.00327 16800.00 10000.00 0.00 0.00409 17000.00 10000.00 0.00 0.00511 17200.00 10000.00 0.00 0.00605 17400.00 10000.00 0.00 0.00689 17600.00 10000.00 0.00 0.00784 17800.00 10000.00 0.00 0.00888 18000.00 10000.00 0.00 0.00991 18200.00 10000.00 0.00 0.01100 18400.00 10000.00 0.00 0.01228 18600.00 10000.00 0.00 0.01350 18800.00 10000.00 0.00 0.01471 19000.00 10000.00 0.00 0.01600 19200.00 10000.00 0.00 0.01728 19400.00 10000.00 0.00 0.01844 19600.00 10000.00 0.00 0.01945 19800.00 10000.00 0.00 0.02060 20000.00 10000.00 0.00 0.02169 20200.00 10000.00 0.00 0.02281 20400.00 10000.00 0.00 0.02417 20600.00 10000.00 0.00 0.02564 20800.00 10000.00 0.00 0.02701 21000.00 10000.00 0.00 0.02830 21200.00 10000.00 0.00 0.02983 21400.00 10000.00 0.00 0.03151 21600.00 10000.00 0.00 0.03326 21800.00 10000.00 0.00 0.03516 22000.00 10000.00 0.00 0.03708 22200.00 10000.00 0.00 0.03910 22400.00 10000.00 0.00 0.04135 22600.00 10000.00 0.00 0.04360 22800.00 10000.00 0.00 0.04519 23000.00 10000.00 0.00 0.04588 23200.00 10000.00 0.00 0.04611 23000.00 10200.00 0.00 0.04618 23200.00 10200.00 0.00 0.04639 23000.00 10400.00 0.00 0.04651 23200.00 10400.00 0.00 0.04671 23000.00 10600.00 0.00 0.04701 23200.00 10600.00 0.00 0.04720 23400.00 10600.00 0.00 0.04721 23000.00 10800.00 0.00 0.04749 23200.00 10800.00 0.00 0.04770 23400.00 10800.00 0.00 0.04770 23000.00 11000.00 0.00 0.04804 23200.00 11000.00 0.00 0.04825 23000.00 11200.00 0.00 0.04858 23200.00 11200.00 0.00 0.04878 23000.00 11400.00 0.00 0.04915 23200.00 11400.00 0.00 0.04935 23000.00 11600.00 0.00 0.04982 23200.00 11600.00 0.00 0.05000 23000.00 11800.00 0.00 0.05043 23200.00 11800.00 0.00 0.05055 23000.00 12000.00 0.00 0.05086 23200.00 12000.00 0.00 0.05096 23000.00 12200.00 0.00 0.05137 23200.00 12200.00 0.00 0.05151 23000.00 12400.00 0.00 0.05206 23200.00 12400.00 0.00 0.05224 23000.00 12600.00 0.00 0.05284 23200.00 12600.00 0.00 0.05302 23000.00 12800.00 0.00 0.05364 23200.00 12800.00 0.00 0.05378 22800.00 13000.00 0.00 0.05379 23000.00 13000.00 0.00 0.05439 23200.00 13000.00 0.00 0.05451 22800.00 13200.00 0.00 0.05469 23000.00 13200.00 0.00 0.05520 23200.00 13200.00 0.00 0.05530 22800.00 13400.00 0.00 0.05531 23000.00 13400.00 0.00 0.05579 23200.00 13400.00 0.00 0.05593 23000.00 13600.00 0.00 0.05632 23200.00 13600.00 0.00 0.05652 22800.00 13800.00 0.00 0.05655 23000.00 13800.00 0.00 0.05718 23200.00 13800.00 0.00 0.05739 22800.00 14000.00 0.00 0.05758 23000.00 14000.00 0.00 0.05819 23200.00 14000.00 0.00 0.05836 22800.00 14200.00 0.00 0.05859 23000.00 14200.00 0.00 0.05917 23200.00 14200.00 0.00 0.05935 22800.00 14400.00 0.00 0.05965 23000.00 14400.00 0.00 0.06026 23200.00 14400.00 0.00 0.06045 22800.00 14600.00 0.00 0.06091 23000.00 14600.00 0.00 0.06150 23200.00 14600.00 0.00 0.06167 22800.00 14800.00 0.00 0.06210 23000.00 14800.00 0.00 0.06267 23200.00 14800.00 0.00 0.06281 22800.00 15000.00 0.00 0.06334 23000.00 15000.00 0.00 0.06396 23200.00 15000.00 0.00 0.06411 22800.00 15200.00 0.00 0.06467 23000.00 15200.00 0.00 0.06534 23200.00 15200.00 0.00 0.06544 22800.00 15400.00 0.00 0.06604 23000.00 15400.00 0.00 0.06669 23200.00 15400.00 0.00 0.06669 22800.00 15600.00 0.00 0.06746 23000.00 15600.00 0.00 0.06802 22800.00 15800.00 0.00 0.06869 23000.00 15800.00 0.00 0.06917 22800.00 16000.00 0.00 0.06998 23000.00 16000.00 0.00 0.07042 22800.00 16200.00 0.00 0.07131 23000.00 16200.00 0.00 0.07174 22800.00 16400.00 0.00 0.07258 23000.00 16400.00 0.00 0.07298 22800.00 16600.00 0.00 0.07376 23000.00 16600.00 0.00 0.07410 22800.00 16800.00 0.00 0.07486 23000.00 16800.00 0.00 0.07515 22800.00 17000.00 0.00 0.07589 23000.00 17000.00 0.00 0.07617 22800.00 17200.00 0.00 0.07696 23000.00 17200.00 0.00 0.07723 22800.00 17400.00 0.00 0.07825 23000.00 17400.00 0.00 0.07849 22800.00 17600.00 0.00 0.07960 23000.00 17600.00 0.00 0.07978 22800.00 17800.00 0.00 0.08090 23000.00 17800.00 0.00 0.08106 22800.00 18000.00 0.00 0.08218 23000.00 18000.00 0.00 0.08234 22800.00 18200.00 0.00 0.08351 23000.00 18200.00 0.00 0.08367 22800.00 18400.00 0.00 0.08473 23000.00 18400.00 0.00 0.08488 22600.00 18600.00 0.00 0.08489 22800.00 18600.00 0.00 0.08604 23000.00 18600.00 0.00 0.08619 22600.00 18800.00 0.00 0.08620 22800.00 18800.00 0.00 0.08733 23000.00 18800.00 0.00 0.08745 22800.00 19000.00 0.00 0.08836 23000.00 19000.00 0.00 0.08846 22800.00 19200.00 0.00 0.08956 23000.00 19200.00 0.00 0.08961 22600.00 19400.00 0.00 0.08966 22800.00 19400.00 0.00 0.09082 23000.00 19400.00 0.00 0.09083 22600.00 19600.00 0.00 0.09108 22800.00 19600.00 0.00 0.09225 22600.00 19800.00 0.00 0.09238 22800.00 19800.00 0.00 0.09353 22600.00 20000.00 0.00 0.09354 22800.00 20000.00 0.00 0.09467 22600.00 20200.00 0.00 0.09491 22800.00 20200.00 0.00 0.09596 22600.00 20400.00 0.00 0.09633 22800.00 20400.00 0.00 0.09725 22600.00 20600.00 0.00 0.09780 22800.00 20600.00 0.00 0.09857 22600.00 20800.00 0.00 0.09910 22800.00 20800.00 0.00 0.09983 22600.00 21000.00 0.00 0.10057 22800.00 21000.00 0.00 0.10128 22600.00 21200.00 0.00 0.10192 22800.00 21200.00 0.00 0.10255 22600.00 21400.00 0.00 0.10314 22800.00 21400.00 0.00 0.10365 22600.00 21600.00 0.00 0.10429 22800.00 21600.00 0.00 0.10468 22600.00 21800.00 0.00 0.10535 22800.00 21800.00 0.00 0.10560 22600.00 22000.00 0.00 0.10649 22800.00 22000.00 0.00 0.10654 22600.00 22200.00 0.00 0.10733 22600.00 22400.00 0.00 0.10832 22600.00 22600.00 0.00 0.10898 22600.00 22800.00 0.00 0.10909 22600.00 22800.00 5.00 0.10915 22600.00 22800.00 10.00 0.10920 22600.00 22800.00 15.00 0.10924 22600.00 22800.00 20.00 0.10926 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 22516.68 22791.57 31.29 0.10940 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/341.ctf NX, NY, NZ: 512 512 1 MODE : real