CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/340.mrc Output diagnostic file name Micrographs_0601/340.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/340.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2444.000 7612.000 Mean, RMS : 4488.722 221.0236 TITLE 1: emi2mrc: 340.emi Sun Jan 05 22:54:16 2014 TITLE 2: kV 300, Mag 78000x, Def 19030, Spot 4, Int 55.07%, Obj 88.09%, Dif 73.67% TITLE 3: X 43.809, Y 588.736, Z -76.743, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.00071 10200.00 10000.00 0.00 -0.00058 10400.00 10000.00 0.00 -0.00037 10600.00 10000.00 0.00 -0.00014 10800.00 10000.00 0.00 -0.00005 11000.00 10000.00 0.00 -0.00001 11200.00 10000.00 0.00 0.00019 11400.00 10000.00 0.00 0.00051 11600.00 10000.00 0.00 0.00087 11800.00 10000.00 0.00 0.00124 12000.00 10000.00 0.00 0.00172 12200.00 10000.00 0.00 0.00215 12400.00 10000.00 0.00 0.00249 12600.00 10000.00 0.00 0.00301 12800.00 10000.00 0.00 0.00348 13000.00 10000.00 0.00 0.00386 13200.00 10000.00 0.00 0.00440 13400.00 10000.00 0.00 0.00496 13600.00 10000.00 0.00 0.00561 13800.00 10000.00 0.00 0.00625 14000.00 10000.00 0.00 0.00681 14200.00 10000.00 0.00 0.00749 14400.00 10000.00 0.00 0.00828 14600.00 10000.00 0.00 0.00911 14800.00 10000.00 0.00 0.00993 15000.00 10000.00 0.00 0.01073 15200.00 10000.00 0.00 0.01163 15400.00 10000.00 0.00 0.01260 15600.00 10000.00 0.00 0.01343 15800.00 10000.00 0.00 0.01428 16000.00 10000.00 0.00 0.01531 16200.00 10000.00 0.00 0.01625 16400.00 10000.00 0.00 0.01716 16600.00 10000.00 0.00 0.01807 16800.00 10000.00 0.00 0.01888 17000.00 10000.00 0.00 0.01982 17200.00 10000.00 0.00 0.02092 17400.00 10000.00 0.00 0.02212 17600.00 10000.00 0.00 0.02337 17800.00 10000.00 0.00 0.02458 18000.00 10000.00 0.00 0.02579 18200.00 10000.00 0.00 0.02708 18400.00 10000.00 0.00 0.02833 18600.00 10000.00 0.00 0.02953 18800.00 10000.00 0.00 0.03085 19000.00 10000.00 0.00 0.03207 19200.00 10000.00 0.00 0.03329 19400.00 10000.00 0.00 0.03464 19600.00 10000.00 0.00 0.03623 19800.00 10000.00 0.00 0.03792 20000.00 10000.00 0.00 0.03959 20200.00 10000.00 0.00 0.04149 20400.00 10000.00 0.00 0.04344 20600.00 10000.00 0.00 0.04553 20800.00 10000.00 0.00 0.04766 21000.00 10000.00 0.00 0.04979 21200.00 10000.00 0.00 0.05207 21400.00 10000.00 0.00 0.05410 21600.00 10000.00 0.00 0.05537 21800.00 10000.00 0.00 0.05586 22000.00 10000.00 0.00 0.05605 22200.00 10000.00 0.00 0.05612 21800.00 10200.00 0.00 0.05644 22000.00 10200.00 0.00 0.05664 22200.00 10200.00 0.00 0.05672 21800.00 10400.00 0.00 0.05716 22000.00 10400.00 0.00 0.05735 22200.00 10400.00 0.00 0.05742 21800.00 10600.00 0.00 0.05770 22000.00 10600.00 0.00 0.05785 22200.00 10600.00 0.00 0.05791 21800.00 10800.00 0.00 0.05808 22000.00 10800.00 0.00 0.05823 22200.00 10800.00 0.00 0.05833 21800.00 11000.00 0.00 0.05858 22000.00 11000.00 0.00 0.05876 22200.00 11000.00 0.00 0.05890 21800.00 11200.00 0.00 0.05906 22000.00 11200.00 0.00 0.05930 22200.00 11200.00 0.00 0.05950 21800.00 11400.00 0.00 0.05968 22000.00 11400.00 0.00 0.05999 22200.00 11400.00 0.00 0.06021 21800.00 11600.00 0.00 0.06039 22000.00 11600.00 0.00 0.06074 22200.00 11600.00 0.00 0.06092 21800.00 11800.00 0.00 0.06123 22000.00 11800.00 0.00 0.06158 22200.00 11800.00 0.00 0.06173 21800.00 12000.00 0.00 0.06212 22000.00 12000.00 0.00 0.06248 22200.00 12000.00 0.00 0.06261 21800.00 12200.00 0.00 0.06302 22000.00 12200.00 0.00 0.06338 22200.00 12200.00 0.00 0.06349 21800.00 12400.00 0.00 0.06392 22000.00 12400.00 0.00 0.06427 22200.00 12400.00 0.00 0.06432 21800.00 12600.00 0.00 0.06470 22000.00 12600.00 0.00 0.06503 22200.00 12600.00 0.00 0.06506 21800.00 12800.00 0.00 0.06559 22000.00 12800.00 0.00 0.06590 22200.00 12800.00 0.00 0.06592 21600.00 13000.00 0.00 0.06599 21800.00 13000.00 0.00 0.06654 22000.00 13000.00 0.00 0.06685 21600.00 13200.00 0.00 0.06706 21800.00 13200.00 0.00 0.06761 22000.00 13200.00 0.00 0.06790 21600.00 13400.00 0.00 0.06815 21800.00 13400.00 0.00 0.06870 22000.00 13400.00 0.00 0.06894 21600.00 13600.00 0.00 0.06930 21800.00 13600.00 0.00 0.06981 22000.00 13600.00 0.00 0.07000 21600.00 13800.00 0.00 0.07030 21800.00 13800.00 0.00 0.07076 22000.00 13800.00 0.00 0.07093 21600.00 14000.00 0.00 0.07120 21800.00 14000.00 0.00 0.07164 22000.00 14000.00 0.00 0.07183 21600.00 14200.00 0.00 0.07230 21800.00 14200.00 0.00 0.07277 22000.00 14200.00 0.00 0.07297 21600.00 14400.00 0.00 0.07348 21800.00 14400.00 0.00 0.07399 22000.00 14400.00 0.00 0.07421 21600.00 14600.00 0.00 0.07492 21800.00 14600.00 0.00 0.07544 22000.00 14600.00 0.00 0.07565 21600.00 14800.00 0.00 0.07635 21800.00 14800.00 0.00 0.07689 22000.00 14800.00 0.00 0.07711 21600.00 15000.00 0.00 0.07779 21800.00 15000.00 0.00 0.07841 22000.00 15000.00 0.00 0.07864 21600.00 15200.00 0.00 0.07948 21800.00 15200.00 0.00 0.08013 22000.00 15200.00 0.00 0.08029 21600.00 15400.00 0.00 0.08125 21800.00 15400.00 0.00 0.08184 22000.00 15400.00 0.00 0.08192 21600.00 15600.00 0.00 0.08291 21800.00 15600.00 0.00 0.08345 22000.00 15600.00 0.00 0.08349 21600.00 15800.00 0.00 0.08445 21800.00 15800.00 0.00 0.08495 22000.00 15800.00 0.00 0.08497 21600.00 16000.00 0.00 0.08603 21800.00 16000.00 0.00 0.08647 21600.00 16200.00 0.00 0.08752 21800.00 16200.00 0.00 0.08791 21600.00 16400.00 0.00 0.08919 21800.00 16400.00 0.00 0.08952 21600.00 16600.00 0.00 0.09076 21800.00 16600.00 0.00 0.09100 21600.00 16800.00 0.00 0.09226 21800.00 16800.00 0.00 0.09245 21600.00 17000.00 0.00 0.09387 21800.00 17000.00 0.00 0.09405 21400.00 17200.00 0.00 0.09407 21600.00 17200.00 0.00 0.09552 21800.00 17200.00 0.00 0.09571 21400.00 17400.00 0.00 0.09596 21600.00 17400.00 0.00 0.09738 21800.00 17400.00 0.00 0.09757 21400.00 17600.00 0.00 0.09774 21600.00 17600.00 0.00 0.09914 21800.00 17600.00 0.00 0.09929 21400.00 17800.00 0.00 0.09968 21600.00 17800.00 0.00 0.10104 21800.00 17800.00 0.00 0.10116 21400.00 18000.00 0.00 0.10165 21600.00 18000.00 0.00 0.10301 21800.00 18000.00 0.00 0.10312 21400.00 18200.00 0.00 0.10367 21600.00 18200.00 0.00 0.10504 21800.00 18200.00 0.00 0.10513 21400.00 18400.00 0.00 0.10591 21600.00 18400.00 0.00 0.10731 21800.00 18400.00 0.00 0.10735 21400.00 18600.00 0.00 0.10820 21600.00 18600.00 0.00 0.10956 21400.00 18800.00 0.00 0.11044 21600.00 18800.00 0.00 0.11175 21400.00 19000.00 0.00 0.11251 21600.00 19000.00 0.00 0.11377 21400.00 19200.00 0.00 0.11465 21600.00 19200.00 0.00 0.11584 21400.00 19400.00 0.00 0.11672 21600.00 19400.00 0.00 0.11782 21400.00 19600.00 0.00 0.11903 21600.00 19600.00 0.00 0.12006 21400.00 19800.00 0.00 0.12158 21600.00 19800.00 0.00 0.12254 21400.00 20000.00 0.00 0.12431 21600.00 20000.00 0.00 0.12515 21400.00 20200.00 0.00 0.12718 21600.00 20200.00 0.00 0.12782 21400.00 20400.00 0.00 0.12995 21600.00 20400.00 0.00 0.13028 21200.00 20600.00 0.00 0.13061 21400.00 20600.00 0.00 0.13293 21200.00 20800.00 0.00 0.13362 21400.00 20800.00 0.00 0.13566 21200.00 21000.00 0.00 0.13672 21400.00 21000.00 0.00 0.13826 21200.00 21200.00 0.00 0.13910 21400.00 21200.00 0.00 0.13995 21200.00 21400.00 10.00 0.14002 21200.00 21400.00 15.00 0.14020 21200.00 21400.00 20.00 0.14036 21200.00 21400.00 25.00 0.14051 21200.00 21400.00 30.00 0.14064 21200.00 21400.00 35.00 0.14074 21200.00 21400.00 40.00 0.14081 21200.00 21400.00 45.00 0.14084 21200.00 21400.00 50.00 0.14085 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 21200.00 21400.00 50.00 0.14085 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/340.ctf NX, NY, NZ: 512 512 1 MODE : real