CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/330.mrc Output diagnostic file name Micrographs_0601/330.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/330.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2470.000 7756.000 Mean, RMS : 4497.643 218.2176 TITLE 1: emi2mrc: 330.emi Sun Jan 05 22:43:11 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 69.921, Y 585.124, Z -75.939, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.04496 10200.00 10000.00 0.00 0.04711 10400.00 10000.00 0.00 0.04910 10600.00 10000.00 0.00 0.05131 10800.00 10000.00 0.00 0.05347 11000.00 10000.00 0.00 0.05610 11200.00 10000.00 0.00 0.05880 11400.00 10000.00 0.00 0.06147 11600.00 10000.00 0.00 0.06451 11800.00 10000.00 0.00 0.06755 12000.00 10000.00 0.00 0.07080 12200.00 10000.00 0.00 0.07391 12400.00 10000.00 0.00 0.07709 12600.00 10000.00 0.00 0.08042 12800.00 10000.00 0.00 0.08369 13000.00 10000.00 0.00 0.08687 13200.00 10000.00 0.00 0.09031 13400.00 10000.00 0.00 0.09400 13600.00 10000.00 0.00 0.09760 13800.00 10000.00 0.00 0.10134 14000.00 10000.00 0.00 0.10417 14200.00 10000.00 0.00 0.10560 14400.00 10000.00 0.00 0.10600 14600.00 10000.00 0.00 0.10604 14000.00 10200.00 0.00 0.10669 14200.00 10200.00 0.00 0.10808 14400.00 10200.00 0.00 0.10836 14000.00 10400.00 0.00 0.10918 14200.00 10400.00 0.00 0.11048 14400.00 10400.00 0.00 0.11064 14000.00 10600.00 0.00 0.11187 14200.00 10600.00 0.00 0.11311 14400.00 10600.00 0.00 0.11323 14000.00 10800.00 0.00 0.11482 14200.00 10800.00 0.00 0.11600 14400.00 10800.00 0.00 0.11602 14000.00 11000.00 0.00 0.11801 14200.00 11000.00 0.00 0.11908 14000.00 11200.00 0.00 0.12100 14200.00 11200.00 0.00 0.12200 14000.00 11400.00 0.00 0.12430 14200.00 11400.00 0.00 0.12521 14000.00 11600.00 0.00 0.12745 14200.00 11600.00 0.00 0.12823 14000.00 11800.00 0.00 0.13077 14200.00 11800.00 0.00 0.13138 13800.00 12000.00 0.00 0.13154 14000.00 12000.00 0.00 0.13410 14200.00 12000.00 0.00 0.13454 13800.00 12200.00 0.00 0.13486 14000.00 12200.00 0.00 0.13733 14200.00 12200.00 0.00 0.13761 13800.00 12400.00 0.00 0.13838 14000.00 12400.00 0.00 0.14077 14200.00 12400.00 0.00 0.14088 13800.00 12600.00 0.00 0.14167 14000.00 12600.00 0.00 0.14393 13800.00 12800.00 0.00 0.14514 14000.00 12800.00 0.00 0.14721 13800.00 13000.00 0.00 0.14828 14000.00 13000.00 0.00 0.15015 13800.00 13200.00 0.00 0.15186 14000.00 13200.00 0.00 0.15343 13800.00 13400.00 0.00 0.15515 14000.00 13400.00 0.00 0.15630 13800.00 13600.00 0.00 0.15825 14000.00 13600.00 0.00 0.15872 13800.00 13800.00 0.00 0.16088 13800.00 14000.00 35.00 0.16094 13800.00 14000.00 40.00 0.16099 13800.00 14000.00 45.00 0.16101 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13800.00 13897.15 53.01 0.16134 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/330.ctf NX, NY, NZ: 512 512 1 MODE : real