CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/329.mrc Output diagnostic file name Micrographs_0601/329.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/329.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2198.000 8010.000 Mean, RMS : 4500.729 219.9176 TITLE 1: emi2mrc: 329.emi Sun Jan 05 22:42:20 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 70.362, Y 587.809, Z -75.939, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02599 10200.00 10000.00 0.00 0.02710 10400.00 10000.00 0.00 0.02810 10600.00 10000.00 0.00 0.02933 10800.00 10000.00 0.00 0.03061 11000.00 10000.00 0.00 0.03201 11200.00 10000.00 0.00 0.03337 11400.00 10000.00 0.00 0.03475 11600.00 10000.00 0.00 0.03632 11800.00 10000.00 0.00 0.03800 12000.00 10000.00 0.00 0.03982 12200.00 10000.00 0.00 0.04156 12400.00 10000.00 0.00 0.04350 12600.00 10000.00 0.00 0.04554 12800.00 10000.00 0.00 0.04776 13000.00 10000.00 0.00 0.05018 13200.00 10000.00 0.00 0.05247 13400.00 10000.00 0.00 0.05486 13600.00 10000.00 0.00 0.05735 13800.00 10000.00 0.00 0.05996 14000.00 10000.00 0.00 0.06253 14200.00 10000.00 0.00 0.06515 14400.00 10000.00 0.00 0.06785 14600.00 10000.00 0.00 0.07061 14800.00 10000.00 0.00 0.07353 15000.00 10000.00 0.00 0.07653 15200.00 10000.00 0.00 0.07979 15400.00 10000.00 0.00 0.08319 15600.00 10000.00 0.00 0.08656 15800.00 10000.00 0.00 0.08906 16000.00 10000.00 0.00 0.09007 16200.00 10000.00 0.00 0.09024 15800.00 10200.00 0.00 0.09069 16000.00 10200.00 0.00 0.09167 16200.00 10200.00 0.00 0.09180 15800.00 10400.00 0.00 0.09240 16000.00 10400.00 0.00 0.09332 16200.00 10400.00 0.00 0.09342 15800.00 10600.00 0.00 0.09422 16000.00 10600.00 0.00 0.09510 16200.00 10600.00 0.00 0.09517 15800.00 10800.00 0.00 0.09606 16000.00 10800.00 0.00 0.09692 16200.00 10800.00 0.00 0.09696 15800.00 11000.00 0.00 0.09805 16000.00 11000.00 0.00 0.09890 16200.00 11000.00 0.00 0.09891 15800.00 11200.00 0.00 0.10006 16000.00 11200.00 0.00 0.10091 15800.00 11400.00 0.00 0.10228 16000.00 11400.00 0.00 0.10314 15800.00 11600.00 0.00 0.10453 16000.00 11600.00 0.00 0.10539 15800.00 11800.00 0.00 0.10698 16000.00 11800.00 0.00 0.10778 15800.00 12000.00 0.00 0.10942 16000.00 12000.00 0.00 0.11011 15800.00 12200.00 0.00 0.11192 16000.00 12200.00 0.00 0.11255 15800.00 12400.00 0.00 0.11455 16000.00 12400.00 0.00 0.11515 15800.00 12600.00 0.00 0.11711 16000.00 12600.00 0.00 0.11764 15800.00 12800.00 0.00 0.11998 16000.00 12800.00 0.00 0.12039 15800.00 13000.00 0.00 0.12265 16000.00 13000.00 0.00 0.12298 15600.00 13200.00 0.00 0.12331 15800.00 13200.00 0.00 0.12563 16000.00 13200.00 0.00 0.12588 15600.00 13400.00 0.00 0.12646 15800.00 13400.00 0.00 0.12872 16000.00 13400.00 0.00 0.12884 15600.00 13600.00 0.00 0.12957 15800.00 13600.00 0.00 0.13177 15600.00 13800.00 0.00 0.13268 15800.00 13800.00 0.00 0.13485 15600.00 14000.00 0.00 0.13576 15800.00 14000.00 0.00 0.13791 15600.00 14200.00 0.00 0.13923 15800.00 14200.00 0.00 0.14131 15600.00 14400.00 0.00 0.14244 15800.00 14400.00 0.00 0.14444 15600.00 14600.00 0.00 0.14598 15800.00 14600.00 0.00 0.14781 15600.00 14800.00 0.00 0.14931 15800.00 14800.00 0.00 0.15089 15600.00 15000.00 0.00 0.15286 15800.00 15000.00 0.00 0.15410 15600.00 15200.00 0.00 0.15649 15800.00 15200.00 0.00 0.15726 15600.00 15400.00 0.00 0.15980 15800.00 15400.00 0.00 0.15987 15600.00 15600.00 0.00 0.16284 15600.00 15800.00 35.00 0.16285 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15600.78 15697.58 36.69 0.16349 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/329.ctf NX, NY, NZ: 512 512 1 MODE : real