CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/327.mrc Output diagnostic file name Micrographs_0601/327.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/327.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2352.000 8884.000 Mean, RMS : 4507.242 216.5746 TITLE 1: emi2mrc: 327.emi Sun Jan 05 22:36:53 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 54.733, Y 589.892, Z -75.939, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02469 10200.00 10000.00 0.00 0.02603 10400.00 10000.00 0.00 0.02742 10600.00 10000.00 0.00 0.02912 10800.00 10000.00 0.00 0.03066 11000.00 10000.00 0.00 0.03239 11200.00 10000.00 0.00 0.03427 11400.00 10000.00 0.00 0.03615 11600.00 10000.00 0.00 0.03802 11800.00 10000.00 0.00 0.03995 12000.00 10000.00 0.00 0.04213 12200.00 10000.00 0.00 0.04419 12400.00 10000.00 0.00 0.04642 12600.00 10000.00 0.00 0.04878 12800.00 10000.00 0.00 0.05120 13000.00 10000.00 0.00 0.05392 13200.00 10000.00 0.00 0.05683 13400.00 10000.00 0.00 0.05991 13600.00 10000.00 0.00 0.06285 13800.00 10000.00 0.00 0.06571 14000.00 10000.00 0.00 0.06864 14200.00 10000.00 0.00 0.07188 14400.00 10000.00 0.00 0.07538 14600.00 10000.00 0.00 0.07901 14800.00 10000.00 0.00 0.08253 15000.00 10000.00 0.00 0.08532 15200.00 10000.00 0.00 0.08681 15400.00 10000.00 0.00 0.08704 15000.00 10200.00 0.00 0.08728 15200.00 10200.00 0.00 0.08875 15400.00 10200.00 0.00 0.08892 15000.00 10400.00 0.00 0.08937 15200.00 10400.00 0.00 0.09085 15400.00 10400.00 0.00 0.09095 15000.00 10600.00 0.00 0.09169 15200.00 10600.00 0.00 0.09311 15400.00 10600.00 0.00 0.09312 15000.00 10800.00 0.00 0.09414 15200.00 10800.00 0.00 0.09549 15000.00 11000.00 0.00 0.09697 15200.00 11000.00 0.00 0.09829 15000.00 11200.00 0.00 0.09965 15200.00 11200.00 0.00 0.10096 15000.00 11400.00 0.00 0.10243 15200.00 11400.00 0.00 0.10369 15000.00 11600.00 0.00 0.10513 15200.00 11600.00 0.00 0.10629 15000.00 11800.00 0.00 0.10800 15200.00 11800.00 0.00 0.10900 15000.00 12000.00 0.00 0.11087 15200.00 12000.00 0.00 0.11178 15000.00 12200.00 0.00 0.11376 15200.00 12200.00 0.00 0.11461 15000.00 12400.00 0.00 0.11690 15200.00 12400.00 0.00 0.11770 15000.00 12600.00 0.00 0.11992 15200.00 12600.00 0.00 0.12069 15000.00 12800.00 0.00 0.12335 15200.00 12800.00 0.00 0.12407 15000.00 13000.00 0.00 0.12669 15200.00 13000.00 0.00 0.12731 14800.00 13200.00 0.00 0.12748 15000.00 13200.00 0.00 0.13011 15200.00 13200.00 0.00 0.13057 14800.00 13400.00 0.00 0.13110 15000.00 13400.00 0.00 0.13366 15200.00 13400.00 0.00 0.13393 14800.00 13600.00 0.00 0.13489 15000.00 13600.00 0.00 0.13731 15200.00 13600.00 0.00 0.13734 14800.00 13800.00 0.00 0.13871 15000.00 13800.00 0.00 0.14095 14800.00 14000.00 0.00 0.14246 15000.00 14000.00 0.00 0.14454 14800.00 14200.00 0.00 0.14682 15000.00 14200.00 0.00 0.14864 14800.00 14400.00 0.00 0.15087 15000.00 14400.00 0.00 0.15230 14800.00 14600.00 0.00 0.15522 15000.00 14600.00 0.00 0.15585 14800.00 14800.00 0.00 0.15829 14800.00 15000.00 0.00 0.15838 14800.00 15000.00 5.00 0.15852 14800.00 15000.00 10.00 0.15864 14800.00 15000.00 15.00 0.15874 14800.00 15000.00 20.00 0.15881 14800.00 15000.00 25.00 0.15887 14800.00 15000.00 30.00 0.15890 14800.00 15000.00 35.00 0.15890 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14771.09 14958.91 35.53 0.15905 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/327.ctf NX, NY, NZ: 512 512 1 MODE : real