CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/324.mrc Output diagnostic file name Micrographs_0601/324.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/324.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2530.000 8632.000 Mean, RMS : 4502.960 214.9352 TITLE 1: emi2mrc: 324.emi Sun Jan 05 22:38:18 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 60.115, Y 588.852, Z -75.939, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02510 10200.00 10000.00 0.00 0.02632 10400.00 10000.00 0.00 0.02747 10600.00 10000.00 0.00 0.02889 10800.00 10000.00 0.00 0.03043 11000.00 10000.00 0.00 0.03202 11200.00 10000.00 0.00 0.03363 11400.00 10000.00 0.00 0.03544 11600.00 10000.00 0.00 0.03736 11800.00 10000.00 0.00 0.03920 12000.00 10000.00 0.00 0.04125 12200.00 10000.00 0.00 0.04339 12400.00 10000.00 0.00 0.04575 12600.00 10000.00 0.00 0.04804 12800.00 10000.00 0.00 0.05022 13000.00 10000.00 0.00 0.05249 13200.00 10000.00 0.00 0.05480 13400.00 10000.00 0.00 0.05718 13600.00 10000.00 0.00 0.05972 13800.00 10000.00 0.00 0.06254 14000.00 10000.00 0.00 0.06528 14200.00 10000.00 0.00 0.06817 14400.00 10000.00 0.00 0.07122 14600.00 10000.00 0.00 0.07438 14800.00 10000.00 0.00 0.07774 15000.00 10000.00 0.00 0.08119 15200.00 10000.00 0.00 0.08479 15400.00 10000.00 0.00 0.08792 15600.00 10000.00 0.00 0.09035 15800.00 10000.00 0.00 0.09174 16000.00 10000.00 0.00 0.09218 16200.00 10000.00 0.00 0.09219 15800.00 10200.00 0.00 0.09348 16000.00 10200.00 0.00 0.09390 15600.00 10400.00 0.00 0.09395 15800.00 10400.00 0.00 0.09530 16000.00 10400.00 0.00 0.09566 15600.00 10600.00 0.00 0.09582 15800.00 10600.00 0.00 0.09714 16000.00 10600.00 0.00 0.09743 15600.00 10800.00 0.00 0.09773 15800.00 10800.00 0.00 0.09901 16000.00 10800.00 0.00 0.09922 15600.00 11000.00 0.00 0.09979 15800.00 11000.00 0.00 0.10102 16000.00 11000.00 0.00 0.10115 15600.00 11200.00 0.00 0.10179 15800.00 11200.00 0.00 0.10296 16000.00 11200.00 0.00 0.10298 15600.00 11400.00 0.00 0.10382 15800.00 11400.00 0.00 0.10493 15600.00 11600.00 0.00 0.10576 15800.00 11600.00 0.00 0.10680 15600.00 11800.00 0.00 0.10796 15800.00 11800.00 0.00 0.10890 15600.00 12000.00 0.00 0.11018 15800.00 12000.00 0.00 0.11102 15600.00 12200.00 0.00 0.11236 15800.00 12200.00 0.00 0.11309 15600.00 12400.00 0.00 0.11457 15800.00 12400.00 0.00 0.11522 15600.00 12600.00 0.00 0.11673 15800.00 12600.00 0.00 0.11732 15600.00 12800.00 0.00 0.11919 15800.00 12800.00 0.00 0.11969 15600.00 13000.00 0.00 0.12151 15800.00 13000.00 0.00 0.12190 15400.00 13200.00 0.00 0.12202 15600.00 13200.00 0.00 0.12394 15800.00 13200.00 0.00 0.12427 15400.00 13400.00 0.00 0.12436 15600.00 13400.00 0.00 0.12623 15800.00 13400.00 0.00 0.12650 15400.00 13600.00 0.00 0.12674 15600.00 13600.00 0.00 0.12856 15800.00 13600.00 0.00 0.12874 15400.00 13800.00 0.00 0.12908 15600.00 13800.00 0.00 0.13082 15800.00 13800.00 0.00 0.13085 15400.00 14000.00 0.00 0.13122 15600.00 14000.00 0.00 0.13282 15400.00 14200.00 0.00 0.13361 15600.00 14200.00 0.00 0.13503 15400.00 14400.00 0.00 0.13546 15600.00 14400.00 0.00 0.13670 15400.00 14600.00 0.00 0.13744 15600.00 14600.00 0.00 0.13847 15400.00 14800.00 0.00 0.13912 15600.00 14800.00 0.00 0.13997 15400.00 15000.00 0.00 0.14084 15600.00 15000.00 0.00 0.14149 15400.00 15200.00 0.00 0.14246 15600.00 15200.00 0.00 0.14288 15400.00 15400.00 0.00 0.14324 15600.00 15400.00 0.00 0.14352 15400.00 15600.00 0.00 0.14385 15400.00 15600.00 5.00 0.14392 15400.00 15600.00 10.00 0.14398 15400.00 15600.00 15.00 0.14404 15400.00 15600.00 20.00 0.14409 15400.00 15600.00 25.00 0.14412 15400.00 15600.00 30.00 0.14414 15400.00 15600.00 35.00 0.14414 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15442.64 15614.43 30.68 0.14418 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/324.ctf NX, NY, NZ: 512 512 1 MODE : real