CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/322.mrc Output diagnostic file name Micrographs_0601/322.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/322.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2632.000 8078.000 Mean, RMS : 4495.272 218.4527 TITLE 1: emi2mrc: 322.emi Sun Jan 05 22:35:51 2014 TITLE 2: kV 300, Mag 78000x, Def 20510, Spot 4, Int 55.07%, Obj 88.08%, Dif 73.67% TITLE 3: X 52.015, Y 590.153, Z -75.939, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03051 10200.00 10000.00 0.00 0.03176 10400.00 10000.00 0.00 0.03283 10600.00 10000.00 0.00 0.03412 10800.00 10000.00 0.00 0.03543 11000.00 10000.00 0.00 0.03694 11200.00 10000.00 0.00 0.03853 11400.00 10000.00 0.00 0.04032 11600.00 10000.00 0.00 0.04233 11800.00 10000.00 0.00 0.04435 12000.00 10000.00 0.00 0.04663 12200.00 10000.00 0.00 0.04894 12400.00 10000.00 0.00 0.05129 12600.00 10000.00 0.00 0.05365 12800.00 10000.00 0.00 0.05607 13000.00 10000.00 0.00 0.05843 13200.00 10000.00 0.00 0.06082 13400.00 10000.00 0.00 0.06337 13600.00 10000.00 0.00 0.06605 13800.00 10000.00 0.00 0.06891 14000.00 10000.00 0.00 0.07150 14200.00 10000.00 0.00 0.07424 14400.00 10000.00 0.00 0.07723 14600.00 10000.00 0.00 0.08030 14800.00 10000.00 0.00 0.08339 15000.00 10000.00 0.00 0.08633 15200.00 10000.00 0.00 0.08912 15400.00 10000.00 0.00 0.09074 15600.00 10000.00 0.00 0.09129 15800.00 10000.00 0.00 0.09155 16000.00 10000.00 0.00 0.09155 15400.00 10200.00 0.00 0.09234 15600.00 10200.00 0.00 0.09288 15800.00 10200.00 0.00 0.09316 16000.00 10200.00 0.00 0.09316 15400.00 10400.00 0.00 0.09410 15600.00 10400.00 0.00 0.09472 15800.00 10400.00 0.00 0.09504 15400.00 10600.00 0.00 0.09611 15600.00 10600.00 0.00 0.09678 15800.00 10600.00 0.00 0.09708 15400.00 10800.00 0.00 0.09829 15600.00 10800.00 0.00 0.09893 15800.00 10800.00 0.00 0.09916 15400.00 11000.00 0.00 0.10079 15600.00 11000.00 0.00 0.10137 15800.00 11000.00 0.00 0.10153 15400.00 11200.00 0.00 0.10314 15600.00 11200.00 0.00 0.10366 15800.00 11200.00 0.00 0.10375 15200.00 11400.00 0.00 0.10393 15400.00 11400.00 0.00 0.10556 15600.00 11400.00 0.00 0.10599 15200.00 11600.00 0.00 0.10622 15400.00 11600.00 0.00 0.10777 15600.00 11600.00 0.00 0.10811 15200.00 11800.00 0.00 0.10869 15400.00 11800.00 0.00 0.11019 15600.00 11800.00 0.00 0.11050 15200.00 12000.00 0.00 0.11146 15400.00 12000.00 0.00 0.11294 15600.00 12000.00 0.00 0.11323 15200.00 12200.00 0.00 0.11425 15400.00 12200.00 0.00 0.11571 15600.00 12200.00 0.00 0.11598 15200.00 12400.00 0.00 0.11732 15400.00 12400.00 0.00 0.11874 15600.00 12400.00 0.00 0.11894 15200.00 12600.00 0.00 0.12046 15400.00 12600.00 0.00 0.12184 15600.00 12600.00 0.00 0.12195 15200.00 12800.00 0.00 0.12384 15400.00 12800.00 0.00 0.12516 15200.00 13000.00 0.00 0.12703 15400.00 13000.00 0.00 0.12825 15200.00 13200.00 0.00 0.13038 15400.00 13200.00 0.00 0.13150 15200.00 13400.00 0.00 0.13387 15400.00 13400.00 0.00 0.13486 15200.00 13600.00 0.00 0.13743 15400.00 13600.00 0.00 0.13826 15200.00 13800.00 0.00 0.14106 15400.00 13800.00 0.00 0.14166 15200.00 14000.00 0.00 0.14457 15400.00 14000.00 0.00 0.14494 15000.00 14200.00 0.00 0.14550 15200.00 14200.00 0.00 0.14833 15400.00 14200.00 0.00 0.14845 15000.00 14400.00 0.00 0.14915 15200.00 14400.00 0.00 0.15190 15000.00 14600.00 0.00 0.15333 15200.00 14600.00 0.00 0.15591 15000.00 14800.00 0.00 0.15725 15200.00 14800.00 0.00 0.15958 15000.00 15000.00 0.00 0.16093 15200.00 15000.00 0.00 0.16267 15200.00 15200.00 0.00 0.16312 15000.00 15200.00 20.00 0.16314 15000.00 15200.00 25.00 0.16324 15000.00 15200.00 30.00 0.16332 15000.00 15200.00 35.00 0.16338 15000.00 15200.00 40.00 0.16342 15000.00 15200.00 45.00 0.16344 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15086.71 15200.00 45.00 0.16381 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/322.ctf NX, NY, NZ: 512 512 1 MODE : real