CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/305.mrc Output diagnostic file name Micrographs_0601/305.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/305.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2464.000 8664.000 Mean, RMS : 4483.415 219.0048 TITLE 1: emi2mrc: 305.emi Sun Jan 05 21:58:35 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 52.464, Y 598.355, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01617 14800.00 10000.00 0.00 -0.01602 15000.00 10000.00 0.00 -0.01590 15200.00 10000.00 0.00 -0.01575 15400.00 10000.00 0.00 -0.01553 15600.00 10000.00 0.00 -0.01546 15800.00 10000.00 0.00 -0.01523 16000.00 10000.00 0.00 -0.01474 16200.00 10000.00 0.00 -0.01431 16400.00 10000.00 0.00 -0.01386 16600.00 10000.00 0.00 -0.01348 16800.00 10000.00 0.00 -0.01305 17000.00 10000.00 0.00 -0.01246 17200.00 10000.00 0.00 -0.01203 17400.00 10000.00 0.00 -0.01152 17600.00 10000.00 0.00 -0.01077 17800.00 10000.00 0.00 -0.01007 18000.00 10000.00 0.00 -0.00931 18200.00 10000.00 0.00 -0.00841 18400.00 10000.00 0.00 -0.00759 18600.00 10000.00 0.00 -0.00674 18800.00 10000.00 0.00 -0.00580 19000.00 10000.00 0.00 -0.00496 19200.00 10000.00 0.00 -0.00406 19400.00 10000.00 0.00 -0.00329 19600.00 10000.00 0.00 -0.00276 19800.00 10000.00 0.00 -0.00214 20000.00 10000.00 0.00 -0.00145 20200.00 10000.00 0.00 -0.00070 20400.00 10000.00 0.00 0.00008 20600.00 10000.00 0.00 0.00094 20800.00 10000.00 0.00 0.00192 21000.00 10000.00 0.00 0.00287 21200.00 10000.00 0.00 0.00380 21400.00 10000.00 0.00 0.00459 21600.00 10000.00 0.00 0.00527 21800.00 10000.00 0.00 0.00600 22000.00 10000.00 0.00 0.00685 22200.00 10000.00 0.00 0.00796 22400.00 10000.00 0.00 0.00918 22600.00 10000.00 0.00 0.01034 22800.00 10000.00 0.00 0.01141 23000.00 10000.00 0.00 0.01262 23200.00 10000.00 0.00 0.01406 23400.00 10000.00 0.00 0.01538 23600.00 10000.00 0.00 0.01662 23800.00 10000.00 0.00 0.01794 24000.00 10000.00 0.00 0.01935 24200.00 10000.00 0.00 0.02102 24400.00 10000.00 0.00 0.02291 24600.00 10000.00 0.00 0.02486 24800.00 10000.00 0.00 0.02698 25000.00 10000.00 0.00 0.02882 25200.00 10000.00 0.00 0.02996 25400.00 10000.00 0.00 0.03053 25600.00 10000.00 0.00 0.03070 25800.00 10000.00 0.00 0.03073 25600.00 10200.00 0.00 0.03080 25400.00 10400.00 0.00 0.03086 25600.00 10400.00 0.00 0.03096 25400.00 10600.00 0.00 0.03102 25600.00 10600.00 0.00 0.03114 25800.00 10600.00 0.00 0.03115 25400.00 10800.00 0.00 0.03128 25600.00 10800.00 0.00 0.03138 25800.00 10800.00 0.00 0.03139 25400.00 11000.00 0.00 0.03156 25600.00 11000.00 0.00 0.03167 25800.00 11000.00 0.00 0.03171 25400.00 11200.00 0.00 0.03186 25600.00 11200.00 0.00 0.03204 25800.00 11200.00 0.00 0.03212 25400.00 11400.00 0.00 0.03246 25600.00 11400.00 0.00 0.03268 25800.00 11400.00 0.00 0.03275 25400.00 11600.00 0.00 0.03313 25600.00 11600.00 0.00 0.03334 25800.00 11600.00 0.00 0.03335 25400.00 11800.00 0.00 0.03382 25600.00 11800.00 0.00 0.03400 25400.00 12000.00 0.00 0.03446 25600.00 12000.00 0.00 0.03454 25400.00 12200.00 0.00 0.03497 25200.00 12400.00 0.00 0.03507 25400.00 12400.00 0.00 0.03550 25200.00 12600.00 0.00 0.03562 25400.00 12600.00 0.00 0.03602 25200.00 12800.00 0.00 0.03621 25400.00 12800.00 0.00 0.03655 25200.00 13000.00 0.00 0.03664 25400.00 13000.00 0.00 0.03689 25200.00 13200.00 0.00 0.03703 25400.00 13200.00 0.00 0.03718 25200.00 13400.00 0.00 0.03733 25400.00 13400.00 0.00 0.03746 25200.00 13600.00 0.00 0.03756 25400.00 13600.00 0.00 0.03772 25200.00 13800.00 0.00 0.03808 25400.00 13800.00 0.00 0.03830 25200.00 14000.00 0.00 0.03874 25400.00 14000.00 0.00 0.03898 25200.00 14200.00 0.00 0.03932 25400.00 14200.00 0.00 0.03955 25200.00 14400.00 0.00 0.03985 25400.00 14400.00 0.00 0.04009 25200.00 14600.00 0.00 0.04056 25400.00 14600.00 0.00 0.04083 25200.00 14800.00 0.00 0.04149 25400.00 14800.00 0.00 0.04173 25200.00 15000.00 0.00 0.04228 25400.00 15000.00 0.00 0.04252 25200.00 15200.00 0.00 0.04299 25400.00 15200.00 0.00 0.04329 25200.00 15400.00 0.00 0.04400 25400.00 15400.00 0.00 0.04432 25200.00 15600.00 0.00 0.04495 25400.00 15600.00 0.00 0.04523 25200.00 15800.00 0.00 0.04581 25400.00 15800.00 0.00 0.04607 25200.00 16000.00 0.00 0.04687 25400.00 16000.00 0.00 0.04711 25200.00 16200.00 0.00 0.04782 25400.00 16200.00 0.00 0.04805 25200.00 16400.00 0.00 0.04886 25400.00 16400.00 0.00 0.04912 25200.00 16600.00 0.00 0.05001 25400.00 16600.00 0.00 0.05027 25200.00 16800.00 0.00 0.05122 25400.00 16800.00 0.00 0.05145 25200.00 17000.00 0.00 0.05240 25400.00 17000.00 0.00 0.05259 25200.00 17200.00 0.00 0.05334 25400.00 17200.00 0.00 0.05355 25200.00 17400.00 0.00 0.05444 25400.00 17400.00 0.00 0.05471 25200.00 17600.00 0.00 0.05564 25400.00 17600.00 0.00 0.05592 25200.00 17800.00 0.00 0.05684 25400.00 17800.00 0.00 0.05708 25200.00 18000.00 0.00 0.05792 25400.00 18000.00 0.00 0.05813 25200.00 18200.00 0.00 0.05890 25400.00 18200.00 0.00 0.05908 25200.00 18400.00 0.00 0.05996 25400.00 18400.00 0.00 0.06013 25000.00 18600.00 0.00 0.06025 25200.00 18600.00 0.00 0.06122 25400.00 18600.00 0.00 0.06140 25000.00 18800.00 0.00 0.06166 25200.00 18800.00 0.00 0.06264 25400.00 18800.00 0.00 0.06284 25000.00 19000.00 0.00 0.06309 25200.00 19000.00 0.00 0.06409 25400.00 19000.00 0.00 0.06430 25000.00 19200.00 0.00 0.06459 25200.00 19200.00 0.00 0.06561 25400.00 19200.00 0.00 0.06583 25000.00 19400.00 0.00 0.06612 25200.00 19400.00 0.00 0.06715 25400.00 19400.00 0.00 0.06736 25000.00 19600.00 0.00 0.06772 25200.00 19600.00 0.00 0.06876 25400.00 19600.00 0.00 0.06889 25000.00 19800.00 0.00 0.06927 25200.00 19800.00 0.00 0.07029 25400.00 19800.00 0.00 0.07030 25000.00 20000.00 0.00 0.07078 25200.00 20000.00 0.00 0.07174 25000.00 20200.00 0.00 0.07214 25200.00 20200.00 0.00 0.07308 25000.00 20400.00 0.00 0.07349 25200.00 20400.00 0.00 0.07441 25000.00 20600.00 0.00 0.07504 25200.00 20600.00 0.00 0.07588 25000.00 20800.00 0.00 0.07648 25200.00 20800.00 0.00 0.07727 25000.00 21000.00 0.00 0.07810 25200.00 21000.00 0.00 0.07889 25000.00 21200.00 0.00 0.07966 25200.00 21200.00 0.00 0.08043 25000.00 21400.00 0.00 0.08123 25200.00 21400.00 0.00 0.08195 25000.00 21600.00 0.00 0.08293 25200.00 21600.00 0.00 0.08356 25000.00 21800.00 0.00 0.08449 25200.00 21800.00 0.00 0.08501 25000.00 22000.00 0.00 0.08609 25200.00 22000.00 0.00 0.08651 25000.00 22200.00 0.00 0.08765 25200.00 22200.00 0.00 0.08805 25000.00 22400.00 0.00 0.08943 25200.00 22400.00 0.00 0.08982 25000.00 22600.00 0.00 0.09122 25200.00 22600.00 0.00 0.09161 25000.00 22800.00 0.00 0.09316 25200.00 22800.00 0.00 0.09352 25000.00 23000.00 0.00 0.09524 25200.00 23000.00 0.00 0.09553 25000.00 23200.00 0.00 0.09734 25200.00 23200.00 0.00 0.09751 24800.00 23400.00 0.00 0.09762 25000.00 23400.00 0.00 0.09953 24800.00 23600.00 0.00 0.09984 25000.00 23600.00 0.00 0.10162 24800.00 23800.00 0.00 0.10209 25000.00 23800.00 0.00 0.10371 24800.00 24000.00 0.00 0.10426 25000.00 24000.00 0.00 0.10574 24800.00 24200.00 0.00 0.10664 25000.00 24200.00 0.00 0.10790 24800.00 24400.00 0.00 0.10873 25000.00 24400.00 0.00 0.10965 24800.00 24600.00 0.00 0.11056 25000.00 24600.00 0.00 0.11099 24800.00 24800.00 0.00 0.11192 24800.00 25000.00 10.00 0.11205 24800.00 25000.00 15.00 0.11223 24800.00 25000.00 20.00 0.11240 24800.00 25000.00 25.00 0.11255 24800.00 25000.00 30.00 0.11267 24800.00 25000.00 35.00 0.11277 24800.00 25000.00 40.00 0.11283 24800.00 25000.00 45.00 0.11285 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 24732.37 25051.50 45.00 0.11300 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/305.ctf NX, NY, NZ: 512 512 1 MODE : real