CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/300.mrc Output diagnostic file name Micrographs_0601/300.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/300.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2500.000 8050.000 Mean, RMS : 4480.407 223.2242 TITLE 1: emi2mrc: 300.emi Sun Jan 05 22:00:19 2014 TITLE 2: kV 300, Mag 78000x, Def 24280, Spot 4, Int 55.08%, Obj 88.06%, Dif 73.67% TITLE 3: X 47.218, Y 598.817, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 -0.01133 16800.00 10000.00 0.00 -0.01132 17000.00 10000.00 0.00 -0.01090 17200.00 10000.00 0.00 -0.01040 17400.00 10000.00 0.00 -0.00989 17600.00 10000.00 0.00 -0.00948 17800.00 10000.00 0.00 -0.00907 18000.00 10000.00 0.00 -0.00869 18200.00 10000.00 0.00 -0.00832 18400.00 10000.00 0.00 -0.00787 18600.00 10000.00 0.00 -0.00746 18800.00 10000.00 0.00 -0.00699 19000.00 10000.00 0.00 -0.00634 19200.00 10000.00 0.00 -0.00572 19400.00 10000.00 0.00 -0.00506 19600.00 10000.00 0.00 -0.00442 19800.00 10000.00 0.00 -0.00388 20000.00 10000.00 0.00 -0.00320 20200.00 10000.00 0.00 -0.00231 20400.00 10000.00 0.00 -0.00140 20600.00 10000.00 0.00 -0.00060 20800.00 10000.00 0.00 0.00003 21000.00 10000.00 0.00 0.00057 21200.00 10000.00 0.00 0.00107 21400.00 10000.00 0.00 0.00158 21600.00 10000.00 0.00 0.00220 21800.00 10000.00 0.00 0.00288 22000.00 10000.00 0.00 0.00370 22200.00 10000.00 0.00 0.00451 22400.00 10000.00 0.00 0.00524 22600.00 10000.00 0.00 0.00609 22800.00 10000.00 0.00 0.00708 23000.00 10000.00 0.00 0.00803 23200.00 10000.00 0.00 0.00890 23400.00 10000.00 0.00 0.00978 23600.00 10000.00 0.00 0.01070 23800.00 10000.00 0.00 0.01157 24000.00 10000.00 0.00 0.01251 24200.00 10000.00 0.00 0.01362 24400.00 10000.00 0.00 0.01479 24600.00 10000.00 0.00 0.01600 24800.00 10000.00 0.00 0.01731 25000.00 10000.00 0.00 0.01870 25200.00 10000.00 0.00 0.02015 25400.00 10000.00 0.00 0.02163 25600.00 10000.00 0.00 0.02340 25800.00 10000.00 0.00 0.02531 26000.00 10000.00 0.00 0.02729 26200.00 10000.00 0.00 0.02934 26400.00 10000.00 0.00 0.03088 26600.00 10000.00 0.00 0.03195 26800.00 10000.00 0.00 0.03256 27000.00 10000.00 0.00 0.03273 26800.00 10200.00 0.00 0.03281 27000.00 10200.00 0.00 0.03296 26800.00 10400.00 0.00 0.03312 27000.00 10400.00 0.00 0.03324 26800.00 10600.00 0.00 0.03341 27000.00 10600.00 0.00 0.03348 26800.00 10800.00 0.00 0.03371 27000.00 10800.00 0.00 0.03378 26800.00 11000.00 0.00 0.03404 27000.00 11000.00 0.00 0.03412 26800.00 11200.00 0.00 0.03427 27000.00 11200.00 0.00 0.03434 26800.00 11400.00 0.00 0.03453 27000.00 11400.00 0.00 0.03458 26800.00 11600.00 0.00 0.03479 27000.00 11600.00 0.00 0.03481 26800.00 11800.00 0.00 0.03503 27000.00 11800.00 0.00 0.03506 26800.00 12000.00 0.00 0.03530 27000.00 12000.00 0.00 0.03535 26800.00 12200.00 0.00 0.03567 27000.00 12200.00 0.00 0.03573 26800.00 12400.00 0.00 0.03611 27000.00 12400.00 0.00 0.03616 26800.00 12600.00 0.00 0.03648 27000.00 12600.00 0.00 0.03649 26800.00 12800.00 0.00 0.03682 26800.00 13000.00 0.00 0.03707 26800.00 13200.00 0.00 0.03734 26600.00 13400.00 0.00 0.03747 26800.00 13400.00 0.00 0.03778 26600.00 13600.00 0.00 0.03795 26800.00 13600.00 0.00 0.03825 26600.00 13800.00 0.00 0.03833 26800.00 13800.00 0.00 0.03862 26600.00 14000.00 0.00 0.03875 26800.00 14000.00 0.00 0.03904 26600.00 14200.00 0.00 0.03928 26800.00 14200.00 0.00 0.03958 26600.00 14400.00 0.00 0.03980 26800.00 14400.00 0.00 0.04010 26600.00 14600.00 0.00 0.04033 26800.00 14600.00 0.00 0.04060 26600.00 14800.00 0.00 0.04079 26800.00 14800.00 0.00 0.04101 26600.00 15000.00 0.00 0.04117 26800.00 15000.00 0.00 0.04140 26600.00 15200.00 0.00 0.04167 26800.00 15200.00 0.00 0.04191 26600.00 15400.00 0.00 0.04223 26800.00 15400.00 0.00 0.04243 26600.00 15600.00 0.00 0.04272 26800.00 15600.00 0.00 0.04286 26600.00 15800.00 0.00 0.04319 26800.00 15800.00 0.00 0.04329 26600.00 16000.00 0.00 0.04366 26800.00 16000.00 0.00 0.04376 26600.00 16200.00 0.00 0.04436 26800.00 16200.00 0.00 0.04450 27000.00 16200.00 0.00 0.04452 26600.00 16400.00 0.00 0.04528 26800.00 16400.00 0.00 0.04544 27000.00 16400.00 0.00 0.04547 26600.00 16600.00 0.00 0.04613 26800.00 16600.00 0.00 0.04632 27000.00 16600.00 0.00 0.04636 26600.00 16800.00 0.00 0.04720 26800.00 16800.00 0.00 0.04742 27000.00 16800.00 0.00 0.04744 26600.00 17000.00 0.00 0.04828 26800.00 17000.00 0.00 0.04844 26600.00 17200.00 0.00 0.04915 26800.00 17200.00 0.00 0.04927 26600.00 17400.00 0.00 0.05005 26800.00 17400.00 0.00 0.05018 26600.00 17600.00 0.00 0.05084 26800.00 17600.00 0.00 0.05097 26600.00 17800.00 0.00 0.05175 26800.00 17800.00 0.00 0.05191 26400.00 18000.00 0.00 0.05197 26600.00 18000.00 0.00 0.05280 26800.00 18000.00 0.00 0.05299 26400.00 18200.00 0.00 0.05300 26600.00 18200.00 0.00 0.05382 26800.00 18200.00 0.00 0.05398 26600.00 18400.00 0.00 0.05463 26800.00 18400.00 0.00 0.05473 26600.00 18600.00 0.00 0.05545 26800.00 18600.00 0.00 0.05557 26400.00 18800.00 0.00 0.05578 26600.00 18800.00 0.00 0.05649 26800.00 18800.00 0.00 0.05664 26400.00 19000.00 0.00 0.05678 26600.00 19000.00 0.00 0.05754 26800.00 19000.00 0.00 0.05776 26400.00 19200.00 0.00 0.05801 26600.00 19200.00 0.00 0.05884 26800.00 19200.00 0.00 0.05908 26400.00 19400.00 0.00 0.05939 26600.00 19400.00 0.00 0.06021 26800.00 19400.00 0.00 0.06040 26400.00 19600.00 0.00 0.06063 26600.00 19600.00 0.00 0.06143 26800.00 19600.00 0.00 0.06159 26400.00 19800.00 0.00 0.06172 26600.00 19800.00 0.00 0.06254 26800.00 19800.00 0.00 0.06274 26400.00 20000.00 0.00 0.06289 26600.00 20000.00 0.00 0.06375 26800.00 20000.00 0.00 0.06393 26400.00 20200.00 0.00 0.06427 26600.00 20200.00 0.00 0.06513 26800.00 20200.00 0.00 0.06527 26400.00 20400.00 0.00 0.06570 26600.00 20400.00 0.00 0.06657 26800.00 20400.00 0.00 0.06668 26400.00 20600.00 0.00 0.06714 26600.00 20600.00 0.00 0.06802 26800.00 20600.00 0.00 0.06809 26400.00 20800.00 0.00 0.06853 26600.00 20800.00 0.00 0.06938 26800.00 20800.00 0.00 0.06942 26400.00 21000.00 0.00 0.07007 26600.00 21000.00 0.00 0.07087 26800.00 21000.00 0.00 0.07088 26400.00 21200.00 0.00 0.07160 26600.00 21200.00 0.00 0.07233 26400.00 21400.00 0.00 0.07296 26600.00 21400.00 0.00 0.07363 26400.00 21600.00 0.00 0.07428 26600.00 21600.00 0.00 0.07493 26400.00 21800.00 0.00 0.07572 26600.00 21800.00 0.00 0.07638 26400.00 22000.00 0.00 0.07736 26600.00 22000.00 0.00 0.07801 26400.00 22200.00 0.00 0.07906 26600.00 22200.00 0.00 0.07967 26400.00 22400.00 0.00 0.08079 26600.00 22400.00 0.00 0.08136 26400.00 22600.00 0.00 0.08237 26600.00 22600.00 0.00 0.08291 26400.00 22800.00 0.00 0.08401 26600.00 22800.00 0.00 0.08453 26400.00 23000.00 0.00 0.08572 26600.00 23000.00 0.00 0.08623 26400.00 23200.00 0.00 0.08749 26600.00 23200.00 0.00 0.08798 26400.00 23400.00 0.00 0.08938 26600.00 23400.00 0.00 0.08984 26400.00 23600.00 0.00 0.09125 26600.00 23600.00 0.00 0.09168 26400.00 23800.00 0.00 0.09327 26600.00 23800.00 0.00 0.09362 26400.00 24000.00 0.00 0.09525 26600.00 24000.00 0.00 0.09554 26200.00 24200.00 0.00 0.09577 26400.00 24200.00 0.00 0.09740 26600.00 24200.00 0.00 0.09762 26200.00 24400.00 0.00 0.09798 26400.00 24400.00 0.00 0.09961 26600.00 24400.00 0.00 0.09977 26200.00 24600.00 0.00 0.10033 26400.00 24600.00 0.00 0.10196 26600.00 24600.00 0.00 0.10201 26200.00 24800.00 0.00 0.10281 26400.00 24800.00 0.00 0.10434 26200.00 25000.00 0.00 0.10520 26400.00 25000.00 0.00 0.10659 26200.00 25200.00 0.00 0.10772 26400.00 25200.00 0.00 0.10897 26200.00 25400.00 0.00 0.11019 26400.00 25400.00 0.00 0.11128 26200.00 25600.00 0.00 0.11275 26400.00 25600.00 0.00 0.11359 26200.00 25800.00 0.00 0.11503 26400.00 25800.00 0.00 0.11555 26200.00 26000.00 0.00 0.11731 26400.00 26000.00 0.00 0.11735 26200.00 26200.00 0.00 0.11900 26200.00 26400.00 20.00 0.11910 26200.00 26400.00 25.00 0.11919 26200.00 26400.00 30.00 0.11927 26200.00 26400.00 35.00 0.11932 26200.00 26400.00 40.00 0.11934 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 26133.06 26353.17 44.31 0.11963 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/300.ctf NX, NY, NZ: 512 512 1 MODE : real