CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/292.mrc Output diagnostic file name Micrographs_0601/292.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/292.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2364.000 8694.000 Mean, RMS : 4448.817 213.3948 TITLE 1: emi2mrc: 292.emi Sun Jan 05 22:08:28 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 50.054, Y 595.345, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02982 10200.00 10000.00 0.00 0.03157 10400.00 10000.00 0.00 0.03329 10600.00 10000.00 0.00 0.03508 10800.00 10000.00 0.00 0.03664 11000.00 10000.00 0.00 0.03830 11200.00 10000.00 0.00 0.04018 11400.00 10000.00 0.00 0.04216 11600.00 10000.00 0.00 0.04453 11800.00 10000.00 0.00 0.04710 12000.00 10000.00 0.00 0.04965 12200.00 10000.00 0.00 0.05217 12400.00 10000.00 0.00 0.05493 12600.00 10000.00 0.00 0.05764 12800.00 10000.00 0.00 0.06043 13000.00 10000.00 0.00 0.06337 13200.00 10000.00 0.00 0.06636 13400.00 10000.00 0.00 0.06955 13600.00 10000.00 0.00 0.07269 13800.00 10000.00 0.00 0.07607 14000.00 10000.00 0.00 0.07974 14200.00 10000.00 0.00 0.08327 14400.00 10000.00 0.00 0.08605 14600.00 10000.00 0.00 0.08801 14800.00 10000.00 0.00 0.08893 14600.00 10200.00 0.00 0.09012 14800.00 10200.00 0.00 0.09107 14600.00 10400.00 0.00 0.09259 14800.00 10400.00 0.00 0.09354 14600.00 10600.00 0.00 0.09506 14800.00 10600.00 0.00 0.09595 14600.00 10800.00 0.00 0.09745 14800.00 10800.00 0.00 0.09829 14600.00 11000.00 0.00 0.10029 14800.00 11000.00 0.00 0.10109 14600.00 11200.00 0.00 0.10300 14800.00 11200.00 0.00 0.10372 14400.00 11400.00 0.00 0.10386 14600.00 11400.00 0.00 0.10582 14800.00 11400.00 0.00 0.10640 14400.00 11600.00 0.00 0.10668 14600.00 11600.00 0.00 0.10854 14800.00 11600.00 0.00 0.10902 14400.00 11800.00 0.00 0.10976 14600.00 11800.00 0.00 0.11159 14800.00 11800.00 0.00 0.11204 14400.00 12000.00 0.00 0.11304 14600.00 12000.00 0.00 0.11485 14800.00 12000.00 0.00 0.11527 14400.00 12200.00 0.00 0.11621 14600.00 12200.00 0.00 0.11797 14800.00 12200.00 0.00 0.11830 14400.00 12400.00 0.00 0.11949 14600.00 12400.00 0.00 0.12118 14800.00 12400.00 0.00 0.12141 14400.00 12600.00 0.00 0.12272 14600.00 12600.00 0.00 0.12434 14800.00 12600.00 0.00 0.12447 14400.00 12800.00 0.00 0.12633 14600.00 12800.00 0.00 0.12783 14800.00 12800.00 0.00 0.12785 14400.00 13000.00 0.00 0.12963 14600.00 13000.00 0.00 0.13104 14400.00 13200.00 0.00 0.13332 14600.00 13200.00 0.00 0.13463 14400.00 13400.00 0.00 0.13707 14600.00 13400.00 0.00 0.13822 14400.00 13600.00 0.00 0.14070 14600.00 13600.00 0.00 0.14163 14200.00 13800.00 0.00 0.14172 14400.00 13800.00 0.00 0.14443 14600.00 13800.00 0.00 0.14504 14200.00 14000.00 0.00 0.14513 14400.00 14000.00 0.00 0.14767 14600.00 14000.00 0.00 0.14773 14200.00 14200.00 0.00 0.14879 14400.00 14200.00 0.00 0.15055 14400.00 14400.00 0.00 0.15070 14200.00 14400.00 20.00 0.15083 14200.00 14400.00 25.00 0.15094 14200.00 14400.00 30.00 0.15103 14200.00 14400.00 35.00 0.15110 14200.00 14400.00 40.00 0.15114 14200.00 14400.00 45.00 0.15117 14200.00 14400.00 50.00 0.15117 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 14271.01 14388.65 50.00 0.15139 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/292.ctf NX, NY, NZ: 512 512 1 MODE : real