CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/291.mrc Output diagnostic file name Micrographs_0601/291.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/291.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2230.000 7912.000 Mean, RMS : 4452.471 214.7206 TITLE 1: emi2mrc: 291.emi Sun Jan 05 22:07:03 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.08%, Dif 73.67% TITLE 3: X 47.705, Y 595.403, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 62 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.02198 10200.00 10000.00 0.00 0.02325 10400.00 10000.00 0.00 0.02435 10600.00 10000.00 0.00 0.02568 10800.00 10000.00 0.00 0.02718 11000.00 10000.00 0.00 0.02870 11200.00 10000.00 0.00 0.03035 11400.00 10000.00 0.00 0.03208 11600.00 10000.00 0.00 0.03399 11800.00 10000.00 0.00 0.03598 12000.00 10000.00 0.00 0.03778 12200.00 10000.00 0.00 0.03959 12400.00 10000.00 0.00 0.04164 12600.00 10000.00 0.00 0.04377 12800.00 10000.00 0.00 0.04619 13000.00 10000.00 0.00 0.04882 13200.00 10000.00 0.00 0.05156 13400.00 10000.00 0.00 0.05416 13600.00 10000.00 0.00 0.05673 13800.00 10000.00 0.00 0.05969 14000.00 10000.00 0.00 0.06247 14200.00 10000.00 0.00 0.06529 14400.00 10000.00 0.00 0.06815 14600.00 10000.00 0.00 0.07115 14800.00 10000.00 0.00 0.07453 15000.00 10000.00 0.00 0.07788 15200.00 10000.00 0.00 0.08124 15400.00 10000.00 0.00 0.08376 15600.00 10000.00 0.00 0.08488 15800.00 10000.00 0.00 0.08515 16000.00 10000.00 0.00 0.08526 15400.00 10200.00 0.00 0.08557 15600.00 10200.00 0.00 0.08668 15800.00 10200.00 0.00 0.08695 16000.00 10200.00 0.00 0.08702 15400.00 10400.00 0.00 0.08736 15600.00 10400.00 0.00 0.08846 15800.00 10400.00 0.00 0.08869 15400.00 10600.00 0.00 0.08919 15600.00 10600.00 0.00 0.09028 15800.00 10600.00 0.00 0.09045 15400.00 10800.00 0.00 0.09117 15600.00 10800.00 0.00 0.09223 15800.00 10800.00 0.00 0.09238 15400.00 11000.00 0.00 0.09334 15600.00 11000.00 0.00 0.09441 15800.00 11000.00 0.00 0.09459 15400.00 11200.00 0.00 0.09544 15600.00 11200.00 0.00 0.09655 15800.00 11200.00 0.00 0.09673 15400.00 11400.00 0.00 0.09775 15600.00 11400.00 0.00 0.09883 15800.00 11400.00 0.00 0.09890 15400.00 11600.00 0.00 0.10002 15600.00 11600.00 0.00 0.10098 15400.00 11800.00 0.00 0.10247 15600.00 11800.00 0.00 0.10336 15400.00 12000.00 0.00 0.10514 15600.00 12000.00 0.00 0.10600 15400.00 12200.00 0.00 0.10807 15600.00 12200.00 0.00 0.10890 15400.00 12400.00 0.00 0.11119 15600.00 12400.00 0.00 0.11192 15400.00 12600.00 0.00 0.11397 15600.00 12600.00 0.00 0.11457 15400.00 12800.00 0.00 0.11682 15600.00 12800.00 0.00 0.11731 15400.00 13000.00 0.00 0.11959 15600.00 13000.00 0.00 0.12001 15200.00 13200.00 0.00 0.12043 15400.00 13200.00 0.00 0.12265 15600.00 13200.00 0.00 0.12295 15200.00 13400.00 0.00 0.12365 15400.00 13400.00 0.00 0.12578 15600.00 13400.00 0.00 0.12592 15200.00 13600.00 0.00 0.12695 15400.00 13600.00 0.00 0.12897 15200.00 13800.00 0.00 0.13037 15400.00 13800.00 0.00 0.13225 15200.00 14000.00 0.00 0.13373 15400.00 14000.00 0.00 0.13547 15200.00 14200.00 0.00 0.13731 15400.00 14200.00 0.00 0.13885 15200.00 14400.00 0.00 0.14060 15400.00 14400.00 0.00 0.14184 15200.00 14600.00 0.00 0.14406 15400.00 14600.00 0.00 0.14493 15200.00 14800.00 0.00 0.14702 15400.00 14800.00 0.00 0.14752 15200.00 15000.00 0.00 0.15015 15200.00 15200.00 0.00 0.15234 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 15276.58 15159.57 -44.30 0.15266 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/291.ctf NX, NY, NZ: 512 512 1 MODE : real