CTF DETERMINATION, V3.5 (9-Mar-2013) Distributed under the GNU General Public License (GPL) Input image file name Micrographs_0601/290.mrc Output diagnostic file name Micrographs_0601/290.ctf CS[mm], HT[kV], AmpCnst, XMAG, DStep[um] 2.0 300.0 0.10 104478.0 14.000 Positive defocus values for underfocus Box, ResMin[A], ResMax[A], dFMin[A], dFMax[A], FStep[A], dAst[A] 512 35.0 3.0 10000.0 40000.0 200.0 0.0 Opening MRC/CCP4 file for READ... File : Micrographs_0601/290.mrc NX, NY, NZ: 4096 4096 1 MODE : real Min, max : 2248.000 7568.000 Mean, RMS : 4435.138 213.8625 TITLE 1: emi2mrc: 290.emi Sun Jan 05 22:04:49 2014 TITLE 2: kV 300, Mag 78000x, Def 21000, Spot 4, Int 55.08%, Obj 88.09%, Dif 73.67% TITLE 3: X 42.054, Y 596.165, Z -75.338, A 0.010 deg, Exposure 1.000 sec READING IMAGE... NX, NY= 4096 4096 TILING IMAGE... Total tiles and number used 64 63 FILTERING POWER SPECTRUM... SEARCHING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 10000.00 10000.00 0.00 0.03944 10200.00 10000.00 0.00 0.04156 10400.00 10000.00 0.00 0.04349 10600.00 10000.00 0.00 0.04595 10800.00 10000.00 0.00 0.04837 11000.00 10000.00 0.00 0.05093 11200.00 10000.00 0.00 0.05364 11400.00 10000.00 0.00 0.05639 11600.00 10000.00 0.00 0.05942 11800.00 10000.00 0.00 0.06268 12000.00 10000.00 0.00 0.06620 12200.00 10000.00 0.00 0.06962 12400.00 10000.00 0.00 0.07319 12600.00 10000.00 0.00 0.07653 12800.00 10000.00 0.00 0.07978 13000.00 10000.00 0.00 0.08344 13200.00 10000.00 0.00 0.08729 13400.00 10000.00 0.00 0.09141 13600.00 10000.00 0.00 0.09570 13800.00 10000.00 0.00 0.09919 14000.00 10000.00 0.00 0.10039 13800.00 10200.00 0.00 0.10151 14000.00 10200.00 0.00 0.10266 13800.00 10400.00 0.00 0.10401 14000.00 10400.00 0.00 0.10512 13800.00 10600.00 0.00 0.10672 14000.00 10600.00 0.00 0.10775 13800.00 10800.00 0.00 0.10962 14000.00 10800.00 0.00 0.11057 13800.00 11000.00 0.00 0.11281 14000.00 11000.00 0.00 0.11367 13800.00 11200.00 0.00 0.11571 14000.00 11200.00 0.00 0.11649 13800.00 11400.00 0.00 0.11899 14000.00 11400.00 0.00 0.11968 13800.00 11600.00 0.00 0.12214 14000.00 11600.00 0.00 0.12275 13800.00 11800.00 0.00 0.12530 14000.00 11800.00 0.00 0.12587 13800.00 12000.00 0.00 0.12874 14000.00 12000.00 0.00 0.12923 13800.00 12200.00 0.00 0.13229 14000.00 12200.00 0.00 0.13261 13600.00 12400.00 0.00 0.13278 13800.00 12400.00 0.00 0.13614 14000.00 12400.00 0.00 0.13624 13600.00 12600.00 0.00 0.13638 13800.00 12600.00 0.00 0.13972 13600.00 12800.00 0.00 0.14032 13800.00 12800.00 0.00 0.14353 13600.00 13000.00 0.00 0.14414 13800.00 13000.00 0.00 0.14715 13600.00 13200.00 0.00 0.14819 13800.00 13200.00 0.00 0.15087 13600.00 13400.00 0.00 0.15183 13800.00 13400.00 0.00 0.15414 13600.00 13600.00 0.00 0.15506 13800.00 13600.00 0.00 0.15691 13600.00 13800.00 0.00 0.15765 13800.00 13800.00 0.00 0.15838 REFINING CTF PARAMETERS... DFMID1 DFMID2 ANGAST CC 13704.57 13833.46 23.81 0.15894 Final Values Opening MRC/CCP4 file for WRITE... File : Micrographs_0601/290.ctf NX, NY, NZ: 512 512 1 MODE : real